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Hello Dave,
If you are using the resonant scanner for photoconversion then I recommend using the regular galvo unit instead. Low photoconversion/photobleaching is an inherent property of resonant scanners. However, since that is how Nikon handles simultaneous photoactivation and imaging I would guess that you are doing that already. In that case anyone with an A1 should be able to help. Unfortunately I cannot because we do not have a 405 laser.
cheers,
TF
Timothy Feinstein, PhD
Postdoctoral Fellow
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA 15261
On May 3, 2011, at 9:21 AM, David Knecht wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
>
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> I am wondering if anyone has had good success photoconverting mEOS or Dendra2 with a Nikon A1R 405 laser. We are having trouble getting significant rapid conversion so I am wondering what others have found for the time/power required to convert these probes. Thanks- Dave
>
> Dr. David Knecht
> Department of Molecular and Cell Biology
> Co-head Flow Cytometry and Confocal Microscopy Facility
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)
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