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Photosensitivity

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Michael Jones

Photosensitivity

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Dear All,

We're trying to build an understanding of the importance of phototoxicity in
cell based assays which require manipulation of samples with light.

For at least some cell types, free radicals (generated from components of
media exposed to light) can cause widespread phenotypic change. This is
often cell death, but more subtle changes can occur which confound
experimental investigation. We have developed non-photoxic media which
substitute for DMEM, Neurobasal and B-27. If you would be interested in
comparing these with your current media to see how this affects your
results, we can provide some media in return for feedback. If you aren't
convinced there will be any difference, please take a look at the data from
other groups:

http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html

Thanks,

Michael

Michael Jones PhD

CEO

Cell Guidance Systems

mcammer

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

And what would the controls be for the effects of the cocktails on the biology?
Regards,
Michael

=========================================================================
Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical Center
Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO SKIRBALL 2nd FLOOR, Back right **
http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michael Jones
Sent: Wednesday, May 13, 2015 11:25 AM
To: [hidden email]
Subject: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

We're trying to build an understanding of the importance of phototoxicity in cell based assays which require manipulation of samples with light.

For at least some cell types, free radicals (generated from components of media exposed to light) can cause widespread phenotypic change. This is often cell death, but more subtle changes can occur which confound experimental investigation. We have developed non-photoxic media which substitute for DMEM, Neurobasal and B-27. If you would be interested in comparing these with your current media to see how this affects your results, we can provide some media in return for feedback. If you aren't convinced there will be any difference, please take a look at the data from other groups:

http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html

Thanks,

Michael

Michael Jones PhD

CEO

Cell Guidance Systems

Michael Jones

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Indeed, any media will exert an effect on the biology of a cell. We've just
looked at conditions and cells where standard media kill cells and the new
media doesn't. Not subtle differences. In cells which aren't killed, the
effects are obviously more subtle and would vary between cells and
experimental conditions. So a better question may be: Are unexpected effects
being seen that can be eliminated when photoxicity is addressed?

Best regards,

Michael

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Cammer, Michael
Sent: Wednesday, May 13, 2015 4:38 PM
To: [hidden email]
Subject: Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

And what would the controls be for the effects of the cocktails on the
biology?
Regards,
Michael

=========================================================================
Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical
Center
Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO
SKIRBALL 2nd FLOOR, Back right **
http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of Michael Jones
Sent: Wednesday, May 13, 2015 11:25 AM
To: [hidden email]
Subject: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

We're trying to build an understanding of the importance of phototoxicity in
cell based assays which require manipulation of samples with light.

For at least some cell types, free radicals (generated from components of
media exposed to light) can cause widespread phenotypic change. This is
often cell death, but more subtle changes can occur which confound
experimental investigation. We have developed non-photoxic media which
substitute for DMEM, Neurobasal and B-27. If you would be interested in
comparing these with your current media to see how this affects your
results, we can provide some media in return for feedback. If you aren't
convinced there will be any difference, please take a look at the data from
other groups:

http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html

Thanks,

Michael

Michael Jones PhD

CEO

Cell Guidance Systems

Jason Miller

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Michael-

I would like to know what types of exposure times and with what wavelengths
(and ultimately, fluences) you are seeing this type of phototoxicity. And
how soon after exposure do you see the phototoxicity. I agree with Michael
that it is hard to switch media when you are working with primary cells (in
my case, primary retinal pigment epithelium) for which people have spent
years working on the right media formulation.

Thanks for bringing this to our attention.
Jason (Miller)
Kellogg Eye Center
University of Michigan

On Wed, May 13, 2015 at 11:46 AM, Michael Jones <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Indeed, any media will exert an effect on the biology of a cell. We've just
> looked at conditions and cells where standard media kill cells and the new
> media doesn't. Not subtle differences. In cells which aren't killed, the
> effects are obviously more subtle and would vary between cells and
> experimental conditions. So a better question may be: Are unexpected
> effects
> being seen that can be eliminated when photoxicity is addressed?
>
> Best regards,
>
> Michael
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On
> Behalf Of Cammer, Michael
> Sent: Wednesday, May 13, 2015 4:38 PM
> To: [hidden email]
> Subject: Re: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> And what would the controls be for the effects of the cocktails on the
> biology?
> Regards,
> Michael
>
> =========================================================================
> Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical
> Center
> Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO
> SKIRBALL 2nd FLOOR, Back right **
> http://ocs.med.nyu.edu/microscopy & http://microscopynotes.com/
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On
> Behalf Of Michael Jones
> Sent: Wednesday, May 13, 2015 11:25 AM
> To: [hidden email]
> Subject: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
>
>
> We're trying to build an understanding of the importance of phototoxicity
> in
> cell based assays which require manipulation of samples with light.
>
> For at least some cell types, free radicals (generated from components of
> media exposed to light) can cause widespread phenotypic change. This is
> often cell death, but more subtle changes can occur which confound
> experimental investigation. We have developed non-photoxic media which
> substitute for DMEM, Neurobasal and B-27. If you would be interested in
> comparing these with your current media to see how this affects your
> results, we can provide some media in return for feedback. If you aren't
> convinced there will be any difference, please take a look at the data from
> other groups:
>
> http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html
>
>
>
> Thanks,
>
>
>
> Michael
>
>
>
> Michael Jones PhD
>
> CEO
>
> Cell Guidance Systems
>
>
>

--

Jason Miller, MD, PhD

University of Michigan Kellogg Eye Center

E-mail: *[hidden email] <[hidden email]> *

Neutrality helps the oppressor, never the victim. Silence encourages the
tormentor, never the tormented. -Elie Wiesel, writer, Nobel laureate (b.
1928)

Michael Jones

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jason,

Apologies for the lateness of the reply. In essence we don't have the raw data for the FACs in terms of actual numbers. It was a collaboration with a lab who were unable to obtain ADULT OPCs from the mouse CNS by FACs. We suggested NEUMO+SOS incubation with digested brain material for 1 hour prior to FACs procedure and to use NEMO+SOS both during and at the end to collect the cells. The cells were then plated into Neurobasal+SOS and allowed to recover and differentiate into oligodendrocytes over 5 days. Without NEUMO+SOS procedure there was no viable cells.

Side scatter and forward scatter settings were used to eliminate dead cells, so what remains should have been viable during FACs but cell viability was lost in the coming days after plating onto coverslips. The flow cytometer was a BD FACs Aria II and the lasers used were the 488 nm and 561 nm lasers. Now the power of light that the lasers can produce is 20 mW and 50 mW respectively but I don't know what the power of light was that the cells received for the brief exposure in the sorter. But it is enough to kill the cells after exposure.

Best regards,

Michael

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jason Miller
Sent: Wednesday, May 13, 2015 6:03 PM
To: [hidden email]
Subject: Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Michael-

I would like to know what types of exposure times and with what wavelengths (and ultimately, fluences) you are seeing this type of phototoxicity. And how soon after exposure do you see the phototoxicity. I agree with Michael that it is hard to switch media when you are working with primary cells (in my case, primary retinal pigment epithelium) for which people have spent years working on the right media formulation.

Thanks for bringing this to our attention.
Jason (Miller)
Kellogg Eye Center
University of Michigan

On Wed, May 13, 2015 at 11:46 AM, Michael Jones <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Indeed, any media will exert an effect on the biology of a cell. We've
> just looked at conditions and cells where standard media kill cells
> and the new media doesn't. Not subtle differences. In cells which
> aren't killed, the effects are obviously more subtle and would vary
> between cells and experimental conditions. So a better question may
> be: Are unexpected effects being seen that can be eliminated when
> photoxicity is addressed?
>
> Best regards,
>
> Michael
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On
> Behalf Of Cammer, Michael
> Sent: Wednesday, May 13, 2015 4:38 PM
> To: [hidden email]
> Subject: Re: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> And what would the controls be for the effects of the cocktails on the
> biology?
> Regards,
> Michael
>
> ======================================================================
> === Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone
> Medical Center
> Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO
> SKIRBALL 2nd FLOOR, Back right **
> http://ocs.med.nyu.edu/microscopy &
> http://microscopynotes.com/
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On
> Behalf Of Michael Jones
> Sent: Wednesday, May 13, 2015 11:25 AM
> To: [hidden email]
> Subject: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear All,
>
>
>
> We're trying to build an understanding of the importance of
> phototoxicity in cell based assays which require manipulation of
> samples with light.
>
> For at least some cell types, free radicals (generated from components
> of media exposed to light) can cause widespread phenotypic change.
> This is often cell death, but more subtle changes can occur which
> confound experimental investigation. We have developed non-photoxic
> media which substitute for DMEM, Neurobasal and B-27. If you would be
> interested in comparing these with your current media to see how this
> affects your results, we can provide some media in return for
> feedback. If you aren't convinced there will be any difference, please
> take a look at the data from other groups:
>
> http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html
>
>
>
> Thanks,
>
>
>
> Michael
>
>
>
> Michael Jones PhD
>
> CEO
>
> Cell Guidance Systems
>
>
>

--

Jason Miller, MD, PhD

University of Michigan Kellogg Eye Center

E-mail: *[hidden email] <[hidden email]> *

Neutrality helps the oppressor, never the victim. Silence encourages the tormentor, never the tormented. -Elie Wiesel, writer, Nobel laureate (b.
1928)

Jason Miller

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Michael-

We may indeed try something like your media at some point. In the meantime,
it would certainly be helpful to me (and maybe others on the list) to know
what other cell culture components might be toxic for which you are seeking
proprietary replacements for. I, for example, work on an epithelial cell,
which requires a range of nanomolar components to maintain polarity and
differentiation - hydrocortisone, thyroid hormone (T3), and a few others.
As well, most complex media have a range of small amine compounds -
polyamines like putresceine, and at least for us (and I know for some
primary neuronal and muscle cultures), taurine (an amino sulfonic acid). I
doubt these play a role in phototoxicity, but my chemistry is rusty, so I
don't know.

Best
-Jason

On Fri, May 22, 2015 at 9:50 AM, Michael Jones <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Jason,
>
> Apologies for the lateness of the reply. In essence we don't have the raw
> data for the FACs in terms of actual numbers. It was a collaboration with a
> lab who were unable to obtain ADULT OPCs from the mouse CNS by FACs. We
> suggested NEUMO+SOS incubation with digested brain material for 1 hour
> prior to FACs procedure and to use NEMO+SOS both during and at the end to
> collect the cells. The cells were then plated into Neurobasal+SOS and
> allowed to recover and differentiate into oligodendrocytes over 5 days.
> Without NEUMO+SOS procedure there was no viable cells.
>
> Side scatter and forward scatter settings were used to eliminate dead
> cells, so what remains should have been viable during FACs but cell
> viability was lost in the coming days after plating onto coverslips. The
> flow cytometer was a BD FACs Aria II and the lasers used were the 488 nm
> and 561 nm lasers. Now the power of light that the lasers can produce is 20
> mW and 50 mW respectively but I don't know what the power of light was that
> the cells received for the brief exposure in the sorter. But it is enough
> to kill the cells after exposure.
>
> Best regards,
>
> Michael
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Jason Miller
> Sent: Wednesday, May 13, 2015 6:03 PM
> To: [hidden email]
> Subject: Re: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Michael-
>
> I would like to know what types of exposure times and with what
> wavelengths (and ultimately, fluences) you are seeing this type of
> phototoxicity. And how soon after exposure do you see the phototoxicity. I
> agree with Michael that it is hard to switch media when you are working
> with primary cells (in my case, primary retinal pigment epithelium) for
> which people have spent years working on the right media formulation.
>
> Thanks for bringing this to our attention.
> Jason (Miller)
> Kellogg Eye Center
> University of Michigan
>
> On Wed, May 13, 2015 at 11:46 AM, Michael Jones <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Indeed, any media will exert an effect on the biology of a cell. We've
> > just looked at conditions and cells where standard media kill cells
> > and the new media doesn't. Not subtle differences. In cells which
> > aren't killed, the effects are obviously more subtle and would vary
> > between cells and experimental conditions. So a better question may
> > be: Are unexpected effects being seen that can be eliminated when
> > photoxicity is addressed?
> >
> > Best regards,
> >
> > Michael
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[hidden email]]
> > On
> > Behalf Of Cammer, Michael
> > Sent: Wednesday, May 13, 2015 4:38 PM
> > To: [hidden email]
> > Subject: Re: Photosensitivity
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > And what would the controls be for the effects of the cocktails on the
> > biology?
> > Regards,
> > Michael
> >
> > ======================================================================
> > === Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone
> > Medical Center
> > Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO
> > SKIRBALL 2nd FLOOR, Back right **
> > http://ocs.med.nyu.edu/microscopy &
> > http://microscopynotes.com/
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[hidden email]]
> > On
> > Behalf Of Michael Jones
> > Sent: Wednesday, May 13, 2015 11:25 AM
> > To: [hidden email]
> > Subject: Photosensitivity
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear All,
> >
> >
> >
> > We're trying to build an understanding of the importance of
> > phototoxicity in cell based assays which require manipulation of
> > samples with light.
> >
> > For at least some cell types, free radicals (generated from components
> > of media exposed to light) can cause widespread phenotypic change.
> > This is often cell death, but more subtle changes can occur which
> > confound experimental investigation. We have developed non-photoxic
> > media which substitute for DMEM, Neurobasal and B-27. If you would be
> > interested in comparing these with your current media to see how this
> > affects your results, we can provide some media in return for
> > feedback. If you aren't convinced there will be any difference, please
> > take a look at the data from other groups:
> >
> > http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html
> >
> >
> >
> > Thanks,
> >
> >
> >
> > Michael
> >
> >
> >
> > Michael Jones PhD
> >
> > CEO
> >
> > Cell Guidance Systems
> >
> >
> >
>
>
> --
>
> Jason Miller, MD, PhD
>
> University of Michigan Kellogg Eye Center
>
> E-mail: *[hidden email] <[hidden email]> *
>
>
> Neutrality helps the oppressor, never the victim. Silence encourages the
> tormentor, never the tormented. -Elie Wiesel, writer, Nobel laureate (b.
> 1928)
>

Michael Jones

Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Jason,

We don't have any other media in our sights at the moment. We'll wait to see what kind of demand we see for the current products before making any further investment in R&D. I'm sure it will be possible to apply the principles to other media.

Best regards,

Michael

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jason Miller
Sent: Friday, May 22, 2015 4:29 PM
To: [hidden email]
Subject: Re: Photosensitivity

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Thanks Michael-

We may indeed try something like your media at some point. In the meantime, it would certainly be helpful to me (and maybe others on the list) to know what other cell culture components might be toxic for which you are seeking proprietary replacements for. I, for example, work on an epithelial cell, which requires a range of nanomolar components to maintain polarity and differentiation - hydrocortisone, thyroid hormone (T3), and a few others.
As well, most complex media have a range of small amine compounds - polyamines like putresceine, and at least for us (and I know for some primary neuronal and muscle cultures), taurine (an amino sulfonic acid). I doubt these play a role in phototoxicity, but my chemistry is rusty, so I don't know.

Best
-Jason

On Fri, May 22, 2015 at 9:50 AM, Michael Jones <[hidden email]>
wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Jason,
>
> Apologies for the lateness of the reply. In essence we don't have the
> raw data for the FACs in terms of actual numbers. It was a
> collaboration with a lab who were unable to obtain ADULT OPCs from the
> mouse CNS by FACs. We suggested NEUMO+SOS incubation with digested
> brain material for 1 hour prior to FACs procedure and to use NEMO+SOS
> both during and at the end to collect the cells. The cells were then
> plated into Neurobasal+SOS and allowed to recover and differentiate into oligodendrocytes over 5 days.
> Without NEUMO+SOS procedure there was no viable cells.
>
> Side scatter and forward scatter settings were used to eliminate dead
> cells, so what remains should have been viable during FACs but cell
> viability was lost in the coming days after plating onto coverslips.
> The flow cytometer was a BD FACs Aria II and the lasers used were the
> 488 nm and 561 nm lasers. Now the power of light that the lasers can
> produce is 20 mW and 50 mW respectively but I don't know what the
> power of light was that the cells received for the brief exposure in
> the sorter. But it is enough to kill the cells after exposure.
>
> Best regards,
>
> Michael
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]
> On Behalf Of Jason Miller
> Sent: Wednesday, May 13, 2015 6:03 PM
> To: [hidden email]
> Subject: Re: Photosensitivity
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Michael-
>
> I would like to know what types of exposure times and with what
> wavelengths (and ultimately, fluences) you are seeing this type of
> phototoxicity. And how soon after exposure do you see the
> phototoxicity. I agree with Michael that it is hard to switch media
> when you are working with primary cells (in my case, primary retinal
> pigment epithelium) for which people have spent years working on the right media formulation.
>
> Thanks for bringing this to our attention.
> Jason (Miller)
> Kellogg Eye Center
> University of Michigan
>
> On Wed, May 13, 2015 at 11:46 AM, Michael Jones
> <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Indeed, any media will exert an effect on the biology of a cell.
> > We've just looked at conditions and cells where standard media kill
> > cells and the new media doesn't. Not subtle differences. In cells
> > which aren't killed, the effects are obviously more subtle and would
> > vary between cells and experimental conditions. So a better question
> > may
> > be: Are unexpected effects being seen that can be eliminated when
> > photoxicity is addressed?
> >
> > Best regards,
> >
> > Michael
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[hidden email]]
> > On
> > Behalf Of Cammer, Michael
> > Sent: Wednesday, May 13, 2015 4:38 PM
> > To: [hidden email]
> > Subject: Re: Photosensitivity
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > And what would the controls be for the effects of the cocktails on
> > the biology?
> > Regards,
> > Michael
> >
> > ====================================================================
> > == === Michael Cammer, Microscopy Core & Skirball Institute, NYU
> > Langone Medical Center
> > Cell: 914-309-3270 ** MY OFFICE HAS MOVED TO
> > SKIRBALL 2nd FLOOR, Back right **
> > http://ocs.med.nyu.edu/microscopy &
> > http://microscopynotes.com/
> >
> >
> > -----Original Message-----
> > From: Confocal Microscopy List
> > [mailto:[hidden email]]
> > On
> > Behalf Of Michael Jones
> > Sent: Wednesday, May 13, 2015 11:25 AM
> > To: [hidden email]
> > Subject: Photosensitivity
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Dear All,
> >
> >
> >
> > We're trying to build an understanding of the importance of
> > phototoxicity in cell based assays which require manipulation of
> > samples with light.
> >
> > For at least some cell types, free radicals (generated from
> > components of media exposed to light) can cause widespread phenotypic change.
> > This is often cell death, but more subtle changes can occur which
> > confound experimental investigation. We have developed non-photoxic
> > media which substitute for DMEM, Neurobasal and B-27. If you would
> > be interested in comparing these with your current media to see how
> > this affects your results, we can provide some media in return for
> > feedback. If you aren't convinced there will be any difference,
> > please take a look at the data from other groups:
> >
> > http://www.cellgs.com/Shop/Cell-Culture-Systems/LiveLight.html
> >
> >
> >
> > Thanks,
> >
> >
> >
> > Michael
> >
> >
> >
> > Michael Jones PhD
> >
> > CEO
> >
> > Cell Guidance Systems
> >
> >
> >
>
>
> --
>
> Jason Miller, MD, PhD
>
> University of Michigan Kellogg Eye Center
>
> E-mail: *[hidden email] <[hidden email]> *
>
>
> Neutrality helps the oppressor, never the victim. Silence encourages
> the tormentor, never the tormented. -Elie Wiesel, writer, Nobel laureate (b.
> 1928)
>

--

Jason Miller, MD, PhD

University of Michigan Kellogg Eye Center

E-mail: *[hidden email] <[hidden email]> *

Neutrality helps the oppressor, never the victim. Silence encourages the tormentor, never the tormented. -Elie Wiesel, writer, Nobel laureate (b.
1928)