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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear listers, I am encountering a problem with my piezo scanning stage (sample scanning) in the microscopy setup. I found that at some discrete line scanning frequencies (lines/s), the grating sample I imaged got some distortions along the fast axis (the horizontal axis in following images). See this link for some images: https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 As you can see, for example, at 30 Hz, it seemed that the grating was splitting into multiple lines; and at 40Hz, they are also pretty bad. I am using a bidirectional sawtooth voltage pattern to feed the piezo. So I was wondering if anyone has similar experience on this? I switched to a new scanning stage, but the distortion still existed, and it did not change as field of view and pixel size. Does anyone have an ideal what might be wrong? Thanks a lot, Lu |
Eric Marino |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Lu, Are the images a single plane or projected images? Eric Marino [hidden email] > On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear listers, > > I am encountering a problem with my piezo scanning stage (sample scanning) > in the microscopy setup. I found that at some discrete line scanning > frequencies (lines/s), the grating sample I imaged got some distortions > along the fast axis (the horizontal axis in following images). See this > link for some images: > https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 > > As you can see, for example, at 30 Hz, it seemed that the grating was > splitting into multiple lines; and at 40Hz, they are also pretty bad. I am > using a bidirectional sawtooth voltage pattern to feed the piezo. So I was > wondering if anyone has similar experience on this? I switched to a new > scanning stage, but the distortion still existed, and it did not change as > field of view and pixel size. Does anyone have an ideal what might be wrong? > > Thanks a lot, > Lu |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Eric, The images are all taken at the fixed sample plane. The sample is a simple grating pattern. I took these images at different scanning speed. Lu ------------------------------------------------------------------ ------------- Lu Yan Nanostructured Fibers and Nonlinear Optics Laboratory Electrical and Computer Engineering Boston University Rm. 505, 8 Saint Mary's St., Boston, MA 02215 +1.617.353.0286 (office) | 617.358.5917 (lab) [hidden email] ------------------------------------------------------------------ ------------- On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Lu, > > Are the images a single plane or projected images? > > Eric Marino > [hidden email] > > > > > On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Dear listers, > > > > I am encountering a problem with my piezo scanning stage (sample > scanning) > > in the microscopy setup. I found that at some discrete line scanning > > frequencies (lines/s), the grating sample I imaged got some distortions > > along the fast axis (the horizontal axis in following images). See this > > link for some images: > > > https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 > > > > As you can see, for example, at 30 Hz, it seemed that the grating was > > splitting into multiple lines; and at 40Hz, they are also pretty bad. I > am > > using a bidirectional sawtooth voltage pattern to feed the piezo. So I > was > > wondering if anyone has similar experience on this? I switched to a new > > scanning stage, but the distortion still existed, and it did not change > as > > field of view and pixel size. Does anyone have an ideal what might be > wrong? > > > > Thanks a lot, > > Lu > |
Eric Marino |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Have you looked at the ramp voltage on an oscilloscope to check for noise? What camera are you using (sCMOS, CCD)? are you decreasing the exposure when you increase the scanning frequency? Eric Marino [hidden email] > On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Eric, > > The images are all taken at the fixed sample plane. The sample is a simple > grating pattern. I took these images at different scanning speed. > > Lu > > ------------------------------------------------------------------ > ------------- > Lu Yan > Nanostructured Fibers and Nonlinear Optics Laboratory > Electrical and Computer Engineering > Boston University > Rm. 505, 8 Saint Mary's St., Boston, MA 02215 > +1.617.353.0286 (office) | 617.358.5917 (lab) > [hidden email] > ------------------------------------------------------------------ > ------------- > > On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi Lu, >> >> Are the images a single plane or projected images? >> >> Eric Marino >> [hidden email] >> >> >> >>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Dear listers, >>> >>> I am encountering a problem with my piezo scanning stage (sample >> scanning) >>> in the microscopy setup. I found that at some discrete line scanning >>> frequencies (lines/s), the grating sample I imaged got some distortions >>> along the fast axis (the horizontal axis in following images). See this >>> link for some images: >>> >> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 >>> >>> As you can see, for example, at 30 Hz, it seemed that the grating was >>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I >> am >>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I >> was >>> wondering if anyone has similar experience on this? I switched to a new >>> scanning stage, but the distortion still existed, and it did not change >> as >>> field of view and pixel size. Does anyone have an ideal what might be >> wrong? >>> >>> Thanks a lot, >>> Lu >> |
Mark Cannell-2 |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I can’t see the image but I wonder about aliasing effects between ramp and sensor sampling rate? HTH Mark On 17/03/2016, at 4:06 pm, Eric Marino <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Have you looked at the ramp voltage on an oscilloscope to check for noise? What camera are you using (sCMOS, CCD)? are you decreasing the exposure when you increase the scanning frequency? > > > Eric Marino > [hidden email] > > > >> On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote: >> >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Hi Eric, >> >> The images are all taken at the fixed sample plane. The sample is a simple >> grating pattern. I took these images at different scanning speed. >> >> Lu >> >> ------------------------------------------------------------------ >> ------------- >> Lu Yan >> Nanostructured Fibers and Nonlinear Optics Laboratory >> Electrical and Computer Engineering >> Boston University >> Rm. 505, 8 Saint Mary's St., Boston, MA 02215 >> +1.617.353.0286 (office) | 617.358.5917 (lab) >> [hidden email] >> ------------------------------------------------------------------ >> ------------- >> >> On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> wrote: >> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your posting. >>> ***** >>> >>> Hi Lu, >>> >>> Are the images a single plane or projected images? >>> >>> Eric Marino >>> [hidden email] >>> >>> >>> >>>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: >>>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images on http://www.imgur.com and include the link in your >>> posting. >>>> ***** >>>> >>>> Dear listers, >>>> >>>> I am encountering a problem with my piezo scanning stage (sample >>> scanning) >>>> in the microscopy setup. I found that at some discrete line scanning >>>> frequencies (lines/s), the grating sample I imaged got some distortions >>>> along the fast axis (the horizontal axis in following images). See this >>>> link for some images: >>>> >>> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 >>>> >>>> As you can see, for example, at 30 Hz, it seemed that the grating was >>>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I >>> am >>>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I >>> was >>>> wondering if anyone has similar experience on this? I switched to a new >>>> scanning stage, but the distortion still existed, and it did not change >>> as >>>> field of view and pixel size. Does anyone have an ideal what might be >>> wrong? >>>> >>>> Thanks a lot, >>>> Lu >>> Mark B. Cannell Ph.D. FRSNZ FISHR Professor of Cardiac Cell Biology School of Physiology & Pharmacology Faculty of Biomedical Sciences University of Bristol Bristol BS8 1TD UK [hidden email] |
In reply to this post by Eric Marino
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Eric, The ramp voltage looked fine on oscilloscope. I was using a photon counting module for the detection. Lu On 2016年3月17日 周四 at 下午1:18 Eric Marino <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Have you looked at the ramp voltage on an oscilloscope to check for noise? > What camera are you using (sCMOS, CCD)? are you decreasing the exposure > when you increase the scanning frequency? > > > Eric Marino > [hidden email] > > > > > On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote: > > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Hi Eric, > > > > The images are all taken at the fixed sample plane. The sample is a > simple > > grating pattern. I took these images at different scanning speed. > > > > Lu > > > > ------------------------------------------------------------------ > > ------------- > > Lu Yan > > Nanostructured Fibers and Nonlinear Optics Laboratory > > Electrical and Computer Engineering > > Boston University > > Rm. 505, 8 Saint Mary's St., Boston, MA 02215 > > +1.617.353.0286 (office) | 617.358.5917 (lab) > > [hidden email] > > ------------------------------------------------------------------ > > ------------- > > > > On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> > wrote: > > > >> ***** > >> To join, leave or search the confocal microscopy listserv, go to: > >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >> Post images on http://www.imgur.com and include the link in your > posting. > >> ***** > >> > >> Hi Lu, > >> > >> Are the images a single plane or projected images? > >> > >> Eric Marino > >> [hidden email] > >> > >> > >> > >>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: > >>> > >>> ***** > >>> To join, leave or search the confocal microscopy listserv, go to: > >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > >>> Post images on http://www.imgur.com and include the link in your > >> posting. > >>> ***** > >>> > >>> Dear listers, > >>> > >>> I am encountering a problem with my piezo scanning stage (sample > >> scanning) > >>> in the microscopy setup. I found that at some discrete line scanning > >>> frequencies (lines/s), the grating sample I imaged got some distortions > >>> along the fast axis (the horizontal axis in following images). See this > >>> link for some images: > >>> > >> > https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 > >>> > >>> As you can see, for example, at 30 Hz, it seemed that the grating was > >>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I > >> am > >>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I > >> was > >>> wondering if anyone has similar experience on this? I switched to a new > >>> scanning stage, but the distortion still existed, and it did not change > >> as > >>> field of view and pixel size. Does anyone have an ideal what might be > >> wrong? > >>> > >>> Thanks a lot, > >>> Lu > >> > |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Mark, My detector sampling rate and the pixel clock are sync'ed via the an AO task and a Counter task in the labview program. So would you separately control the sampling rates for the ramp and the sensor? Thanks, Lu On 2016年3月17日 周四 at 下午3:37 Yan, Lu <[hidden email]> wrote: > Hi Eric, > > The ramp voltage looked fine on oscilloscope. I was using a photon > counting module for the detection. > > Lu > > On 2016年3月17日 周四 at 下午1:18 Eric Marino <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Have you looked at the ramp voltage on an oscilloscope to check for >> noise? What camera are you using (sCMOS, CCD)? are you decreasing the >> exposure when you increase the scanning frequency? >> >> >> Eric Marino >> [hidden email] >> >> >> >> > On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote: >> > >> > ***** >> > To join, leave or search the confocal microscopy listserv, go to: >> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> > Post images on http://www.imgur.com and include the link in your >> posting. >> > ***** >> > >> > Hi Eric, >> > >> > The images are all taken at the fixed sample plane. The sample is a >> simple >> > grating pattern. I took these images at different scanning speed. >> > >> > Lu >> > >> > ------------------------------------------------------------------ >> > ------------- >> > Lu Yan >> > Nanostructured Fibers and Nonlinear Optics Laboratory >> > Electrical and Computer Engineering >> > Boston University >> > Rm. 505, 8 Saint Mary's St., Boston, MA 02215 >> > +1.617.353.0286 (office) | 617.358.5917 (lab) >> > [hidden email] >> > ------------------------------------------------------------------ >> > ------------- >> > >> > On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> >> wrote: >> > >> >> ***** >> >> To join, leave or search the confocal microscopy listserv, go to: >> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >> Post images on http://www.imgur.com and include the link in your >> posting. >> >> ***** >> >> >> >> Hi Lu, >> >> >> >> Are the images a single plane or projected images? >> >> >> >> Eric Marino >> >> [hidden email] >> >> >> >> >> >> >> >>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: >> >>> >> >>> ***** >> >>> To join, leave or search the confocal microscopy listserv, go to: >> >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> >>> Post images on http://www.imgur.com and include the link in your >> >> posting. >> >>> ***** >> >>> >> >>> Dear listers, >> >>> >> >>> I am encountering a problem with my piezo scanning stage (sample >> >> scanning) >> >>> in the microscopy setup. I found that at some discrete line scanning >> >>> frequencies (lines/s), the grating sample I imaged got some >> distortions >> >>> along the fast axis (the horizontal axis in following images). See >> this >> >>> link for some images: >> >>> >> >> >> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 >> >>> >> >>> As you can see, for example, at 30 Hz, it seemed that the grating was >> >>> splitting into multiple lines; and at 40Hz, they are also pretty bad. >> I >> >> am >> >>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I >> >> was >> >>> wondering if anyone has similar experience on this? I switched to a >> new >> >>> scanning stage, but the distortion still existed, and it did not >> change >> >> as >> >>> field of view and pixel size. Does anyone have an ideal what might be >> >> wrong? >> >>> >> >>> Thanks a lot, >> >>> Lu >> >> >> > |
Eric Marino |
In reply to this post by Lu Yan
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** I might agree with Mark, it could be a sampling issue. Eric Marino [hidden email] > On Mar 17, 2016, at 3:37 PM, Yan, Lu <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Eric, > > The ramp voltage looked fine on oscilloscope. I was using a photon counting > module for the detection. > > Lu > > On 2016年3月17日 周四 at 下午1:18 Eric Marino <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Have you looked at the ramp voltage on an oscilloscope to check for noise? >> What camera are you using (sCMOS, CCD)? are you decreasing the exposure >> when you increase the scanning frequency? >> >> >> Eric Marino >> [hidden email] >> >> >> >>> On Mar 17, 2016, at 10:59 AM, Yan, Lu <[hidden email]> wrote: >>> >>> ***** >>> To join, leave or search the confocal microscopy listserv, go to: >>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>> Post images on http://www.imgur.com and include the link in your >> posting. >>> ***** >>> >>> Hi Eric, >>> >>> The images are all taken at the fixed sample plane. The sample is a >> simple >>> grating pattern. I took these images at different scanning speed. >>> >>> Lu >>> >>> ------------------------------------------------------------------ >>> ------------- >>> Lu Yan >>> Nanostructured Fibers and Nonlinear Optics Laboratory >>> Electrical and Computer Engineering >>> Boston University >>> Rm. 505, 8 Saint Mary's St., Boston, MA 02215 >>> +1.617.353.0286 (office) | 617.358.5917 (lab) >>> [hidden email] >>> ------------------------------------------------------------------ >>> ------------- >>> >>> On Thu, Mar 17, 2016 at 10:37 AM, Eric Marino <[hidden email]> >> wrote: >>> >>>> ***** >>>> To join, leave or search the confocal microscopy listserv, go to: >>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>> Post images on http://www.imgur.com and include the link in your >> posting. >>>> ***** >>>> >>>> Hi Lu, >>>> >>>> Are the images a single plane or projected images? >>>> >>>> Eric Marino >>>> [hidden email] >>>> >>>> >>>> >>>>> On Mar 17, 2016, at 10:08 AM, Yan, Lu <[hidden email]> wrote: >>>>> >>>>> ***** >>>>> To join, leave or search the confocal microscopy listserv, go to: >>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >>>>> Post images on http://www.imgur.com and include the link in your >>>> posting. >>>>> ***** >>>>> >>>>> Dear listers, >>>>> >>>>> I am encountering a problem with my piezo scanning stage (sample >>>> scanning) >>>>> in the microscopy setup. I found that at some discrete line scanning >>>>> frequencies (lines/s), the grating sample I imaged got some distortions >>>>> along the fast axis (the horizontal axis in following images). See this >>>>> link for some images: >>>>> >>>> >> https://www.dropbox.com/s/9r0e2rea8oaepgi/Screenshot%20-%203_17_2016%20%2C%209_49_00%20AM.png?dl=0 >>>>> >>>>> As you can see, for example, at 30 Hz, it seemed that the grating was >>>>> splitting into multiple lines; and at 40Hz, they are also pretty bad. I >>>> am >>>>> using a bidirectional sawtooth voltage pattern to feed the piezo. So I >>>> was >>>>> wondering if anyone has similar experience on this? I switched to a new >>>>> scanning stage, but the distortion still existed, and it did not change >>>> as >>>>> field of view and pixel size. Does anyone have an ideal what might be >>>> wrong? >>>>> >>>>> Thanks a lot, >>>>> Lu >>>> >> |
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