Please, a question about sCMOS camera adapters for old microscopes. Thank you.

[SANCHEZ MARTIN, CARLOS]

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Hi, good afternoon.

 

We would want to couple an ORCA-Flash 4.0 LT sCMOS camera to a lateral port
of an old Axiovert 200M Zeiss microscope. The dimensions of the chip is
above 1” (13.312 mm x 13.312 mm). In this link you will find the chip
specifications:
http://www2.cbm.uam.es/~smoc/Temp/ORCA-Flash4.0%20LT_sCMOS.pdf

 

Zeiss has told us:

 

““The optimal adapter would be the 1x adapter 60C. With this adapter the
customer gets 18 mm diagonal from the intermediate image (which has a
diameter of 23 mm). Any remaining  shading after proper setting of the
microscope (Koehler) should be corrected with  a shading correction function
of the software.

These old 60 adapters insert into the lightpath diameter and therefore take
away some light. This is one of the reasons for a shading. Therefore the 60N
adapters with the cap screw which leaves the entire lightpath open were
introduced more than 10 years ago for the new microscope generation.
Unfortunately they cannot be attached to Axiovert 200.”

 

Though they advise to use his 1x adapter, my impression is that it is not
going to be enough and we will have to use Shading correction anyway. We
believe that 1.2x adapter might be enough but we have been offered until
1,6x adapter from Best Scientific Couplers
(https://www.bestscientificcouplers.com). Our Zeiss objectives have a Field
of View of 25 mm.

 

I would appreciate a lot your advises, ideas, impressions… whatever. J Do
you think that the 1,2x adapter could be enough? Disadvantages of using 1,6x
adapters in this case?

 

Thank you very much in advance.

Regards,

 

Carlos Sánchez Martín

Servicio de Microscopía Óptica y Confocal (SMOC) (Lab. 310)

(Optical and Confocal Microscopy Facility)

Centro de Biología Molecular Severo Ochoa (UAM-CSIC)

C/Nicolás Cabrera, 1. Universidad Autónoma de Madrid

Cantoblanco. E-28049. Madrid. Spain

Tlf. 34-91 196 4613/4643

Fax. [phone]

E-mail:  <mailto:[hidden email]/[hidden email]>
[hidden email]/[hidden email]

Blog:  <http://csanchezmad.wordpress.com/> http://csanchezmad.wordpress.com

Web:  <http://www.cbm.uam.es/confocal> http://www.cbm.uam.es/confocal

 

Red Española de Microscopía Óptica Avanzada (REMOA)

(Spanish Network of Advanced Optical Microscopy)

 <http://remoa.wikispaces.com/> http://remoa.wikispaces.com

 <http://remoa.net/> http://remoa.net 

 

Plataforma de Microscopía para Biociencias

Red de laboratorios de la Comunidad de Madrid

 <http://www.madrimasd.org/Laboratorios/plataformas-red/ficha.asp?IdPreli=3>
http://www.madrimasd.org/Laboratorios/plataformas-red/ficha.asp?IdPreli=3

 

[Zdenek Svindrych-2]

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Dear Carlos,
with 1.6x adapter you may avoid vignetting, but your field of view will be
smaller. It depends on your application, but given that modern CMOS cameras
have lots of pixels (4Mpix in your case) I would personally prefer larger
FOV. I can always crop the image to "zoom in" the central region...

Also, I believe that the right sideport of our Axiovert 200M can accept both
the 60C adapters (30 mm outer diameter) and the bigger 60N with the 52mm
thread, like this one:

http://www.ebay.com/itm/ZEISS-426114-TRINOCULAR-MICROSCOPE-PHOTOTUBE-TO-C-
MOUNT-ADAPTER-Axio-Imager-Axio-/111221922546?hash=item19e557daf2

Good luck!

zdenek

--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
University of Virginia, Charlottesville, VA
http://www.kcci.virginia.edu/
tel: [phone]


---------- Původní zpráva ----------
Od: Carlos Sanchez Martin <[hidden email]>
Komu: [hidden email]
Datum: 30. 9. 2015 8:31:10
Předmět: Please, a question about sCMOS camera adapters for old microscopes.
Thank you.

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, good afternoon.

We would want to couple an ORCA-Flash 4.0 LT sCMOS camera to a lateral port
of an old Axiovert 200M Zeiss microscope. The dimensions of the chip is
above 1 (13.312 mm x 13.312 mm). In this link you will find the chip
specifications:
http://www2.cbm.uam.es/~smoc/Temp/ORCA-Flash4.0%20LT_sCMOS.pdf

Zeiss has told us:

The optimal adapter would be the 1x adapter 60C. With this adapter the
customer gets 18 mm diagonal from the intermediate image (which has a
diameter of 23 mm). Any remaining shading after proper setting of the
microscope (Koehler) should be corrected with a shading correction function
of the software.

These old 60 adapters insert into the lightpath diameter and therefore take
away some light. This is one of the reasons for a shading. Therefore the 60N
adapters with the cap screw which leaves the entire lightpath open were
introduced more than 10 years ago for the new microscope generation.
Unfortunately they cannot be attached to Axiovert 200.

Though they advise to use his 1x adapter, my impression is that it is not
going to be enough and we will have to use Shading correction anyway. We
believe that 1.2x adapter might be enough but we have been offered until
1,6x adapter from Best Scientific Couplers
(https://www.bestscientificcouplers.com). Our Zeiss objectives have a Field
of View of 25 mm.

I would appreciate a lot your advises, ideas, impressions whatever. J Do
you think that the 1,2x adapter could be enough? Disadvantages of using 1,6x
adapters in this case?

Thank you very much in advance.
Regards,

Carlos Sánchez Martín

"

[WAINWRIGHT James]

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*****

*** COMMERCIAL RESPONSE ***

Hi Carlos,

For 40x or higher magnifications, I agree with Zeiss' recommendation (assuming it's a 1x C-mount with no lens).

I would only use a magnification C-mount (anything up to 2x) if I was doing a lot of low magnification work and wanted to achieve the best lateral resolution (i.e. Nyquist).

Kurt has a good blog post about it here:

https://nic.ucsf.edu/blog/?p=1

In our confocal (spinning disk) systems, we almost always use a 1x adapter for our sCMOS cameras. Using magnification is disadvantageous because of the "dilution" of photos onto the sensor and empty magnification due to small pixel size already achieving Nyquist (at high magnifications).

Hope this helps!

James
________________________________________
From: Confocal Microscopy List [[hidden email]] on behalf of Zdenek Svindrych [[hidden email]]
Sent: 30 September 2015 14:18
To: [hidden email]
Subject: Re: Please, a question about sCMOS camera adapters  for old microscopes. Thank you.

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear Carlos,
with 1.6x adapter you may avoid vignetting, but your field of view will be
smaller. It depends on your application, but given that modern CMOS cameras
have lots of pixels (4Mpix in your case) I would personally prefer larger
FOV. I can always crop the image to "zoom in" the central region...

Also, I believe that the right sideport of our Axiovert 200M can accept both
the 60C adapters (30 mm outer diameter) and the bigger 60N with the 52mm
thread, like this one:

http://www.ebay.com/itm/ZEISS-426114-TRINOCULAR-MICROSCOPE-PHOTOTUBE-TO-C-
MOUNT-ADAPTER-Axio-Imager-Axio-/111221922546?hash=item19e557daf2

Good luck!

zdenek

--
Zdenek Svindrych, Ph.D.
W.M. Keck Center for Cellular Imaging (PLSB 003)
University of Virginia, Charlottesville, VA
http://www.kcci.virginia.edu/
tel: [phone]


---------- Původní zpráva ----------
Od: Carlos Sanchez Martin <[hidden email]>
Komu: [hidden email]
Datum: 30. 9. 2015 8:31:10
Předmět: Please, a question about sCMOS camera adapters for old microscopes.
Thank you.

"*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi, good afternoon.

We would want to couple an ORCA-Flash 4.0 LT sCMOS camera to a lateral port
of an old Axiovert 200M Zeiss microscope. The dimensions of the chip is
above 1 (13.312 mm x 13.312 mm). In this link you will find the chip
specifications:
http://www2.cbm.uam.es/~smoc/Temp/ORCA-Flash4.0%20LT_sCMOS.pdf

Zeiss has told us:

The optimal adapter would be the 1x adapter 60C. With this adapter the
customer gets 18 mm diagonal from the intermediate image (which has a
diameter of 23 mm). Any remaining shading after proper setting of the
microscope (Koehler) should be corrected with a shading correction function
of the software.

These old 60 adapters insert into the lightpath diameter and therefore take
away some light. This is one of the reasons for a shading. Therefore the 60N
adapters with the cap screw which leaves the entire lightpath open were
introduced more than 10 years ago for the new microscope generation.
Unfortunately they cannot be attached to Axiovert 200.

Though they advise to use his 1x adapter, my impression is that it is not
going to be enough and we will have to use Shading correction anyway. We
believe that 1.2x adapter might be enough but we have been offered until
1,6x adapter from Best Scientific Couplers
(https://www.bestscientificcouplers.com). Our Zeiss objectives have a Field
of View of 25 mm.

I would appreciate a lot your advises, ideas, impressions whatever. J Do
you think that the 1,2x adapter could be enough? Disadvantages of using 1,6x
adapters in this case?

Thank you very much in advance.
Regards,

Carlos Sánchez Martín

"


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