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Dear Confocal Listserv,
Please send me fluorescence data of fluorophores and detectors that you
would like to see in the update of a chapter on (light) Microscopy and
Image Analysis
http://www.ncbi.nlm.nih.gov/pubmed/18428379http://onlinelibrary.wiley.com/doi/10.1002/0471142905.hg0404s46/abstractA coauthor posted the PDF on their ResearchGate account,
https://www.researchgate.net/publication/5425300_Current_Protocols_in_Human_GeneticsThe editors have given me flexibility on what to put in -- as long as
the URLs are all updated. #1 on my list is a link to this listserv.
I would like vendors help getting detector quantum efficiency curves in
- I anticipate replacing the CCD pixels figure.
I would very much like to have the Extinction Coefficient's and Quantum
Yields - and of course excitation and emission wavelength maxima, for
all the new fluorophores, such as Brilliant Violet's, Brilliant
Ultraviolet's, Abberior, and STORM/PALM dyes. I want to keep the
table(s) simple, so the best way to send me data is by email, in a
consistent table like this (comma or tab delimited):
Protein λex λem EC QY Reference
Azurite 383 450 26200 0.55 2
EBFP2 383 448 32000 0.56 3
mKalama1 385 456 36000 0.45 3
mTagBFP2 399 454 50600 0.64 4
TagBFP 402 457 52000 0.63 7
For fluorescent proteins, I anticipate including the best FPs from Kurt
Thorn's FP Properties blog (source of the above data)
http://nic.ucsf.edu/FPvisualization/and of course include the web link.
I can now put micrographs into the chapter - the most obvious deficiency
from the previous version.
sincerely,
George
[hidden email]
--
George McNamara, Ph.D.
Houston, TX 77054
[hidden email]
713-239-0365 home
305-764-2081 cell
https://www.linkedin.com/in/georgemcnamarahttps://works.bepress.com/gmcnamara/75/ Tattletales and T-Bow
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