Pollen grain
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Dear list
we had a correction for our diode laser beam and I would like to test the image with some pollen grain,and specially Hoheria sp pollen grain.
Does anyone know any company that has the prepared hoheria pollen grain slide?
I appreciate any information or suggestion.
Regards
Sarah
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These three sites sell prepared biology slides, including ones with pollens... don't know if any of them contains your Hoheria: -- Julio Vazquez Fred Hutchinson Cancer Research Center Seattle, WA 98109-1024 On Jun 13, 2008, at 8:11 AM, Sarah Kefayati wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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https://www.triarchmicroslides.com/search.aspx?keyword=pollen&Search=Search You might contact them directly as they very often have materials not on the site. The question I have is why that species of pollen?!? Julio Vazquez <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal = |
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I was interested to image a round spiky object in 3-D and I know hoheria pollen grain provides this shape.
If any one knows any other specimen ...
On Fri, Jun 13, 2008 at 10:39 PM, Christian <[hidden email]> wrote:
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OH! Sure! Many, and I mean many members in the Asteracea are "spiked". Just find yourself a sunflower (Helianthus sp.) and mount up the pollen. If you really want something interesting, try dandelion pollen (Taraxacum).
Good luck. Sarah Kefayati <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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In reply to this post by Sarah Kefayati
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On 14/6/08 1:16 PM, "Sarah Kefayati" <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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In reply to this post by Sarah Kefayati
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Although I cannot name the species of pollen grains on their slides, I have used numerous prepared slides of pollen grains from Carolina Biological over the years and every single one has had many spiky pollen grains....
No connection to Carolina Biological, except being a happy cuatomer. Chris Tully On Fri, Jun 13, 2008 at 11:16 PM, Sarah Kefayati <[hidden email]> wrote:
-- Chris Tully Microscopy and Image Analysis Expert [hidden email] 240-888-1021 http://www.linkedin.com/in/christully |
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Hi Sarah, I recommend that you take a day (or some hours) off and take a walk to collect pollen yourself and simply embed them in your preferred mounting medium for fluorescent specimens. I promise that you'll have fun and gonna find spiky pollen among other nice structures. If you know a beekeeper don't dare asking for pollen, they collect pollen to determine the bee crop and have amazing collections. It took me one hour to get the specimens I work with 'til today ;-) Why spend money if you can get it for free (including a nice walk in the sunshine) Cheers, Christian
-- ...................................................................... Dr. Christian M. Müller Clinical Neuroanatomy JWG University D-60528 Frankfurt/M., Germany |
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal This spring I've been walking around my suburban neighborhood with a box of slides and clear nailpolish. I spread some nailpolish on a slide and then tap a flower over it oer even press the flower's center into the nailpolish. There's a huge variety of pollen and a few of the results can be found at http://flickr.com/search/?w=39998519%40N00&q=pollen&m=text -Michael > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > Hi Sarah, > I recommend that you take a day (or some hours) off and take a walk to > collect pollen yourself and simply embed them in your preferred mounting > medium for fluorescent specimens. I promise that you'll have fun and > gonna find spiky pollen among other nice structures. If you know a > beekeeper don't dare asking for pollen, they collect pollen to determine > the bee crop and have amazing collections. > It took me one hour to get the specimens I work with 'til today ;-) > > Why spend money if you can get it for free (including a nice walk in the > sunshine) > Cheers, > Christian >> >> >> On Jun 13, 2008, at 8:11 AM, Sarah Kefayati wrote: >>> I would like to test the image with some pollen grain >> > > -- > ...................................................................... > Dr. Christian M. Müller > Clinical Neuroanatomy > JWG University > D-60528 Frankfurt/M., Germany > > > _________________________________________ Michael Cammer http://www.aecom.yu.edu/aif/ |
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In reply to this post by Chris Tully
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Same here. I use Carolina's mixed pollen grain slide all the time. It's great for training, especially for z-stacks, and I always choose the spiky ones to image. The slides are less than $5.00 each. (No connection to Carolina.) I also keep one of the pollen grain slides in the confocal room. When a user says that there's something wrong with the scope, I ask them if they've checked will the pollen slide. Since they inevitably haven't, I tell them to do that first, and then come back if there's a problem with the microscope. Amazingly, they rarely return. Kristi DeCourcy Quoting Chris Tully <[hidden email]>: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Although I cannot name the species of pollen grains on their slides, I have > used numerous prepared slides of pollen grains from Carolina Biological over > the years and every single one has had many spiky pollen grains.... > > No connection to Carolina Biological, except being a happy cuatomer. > > Chris Tully > > On Fri, Jun 13, 2008 at 11:16 PM, Sarah Kefayati <[hidden email]> wrote: > > > Search the CONFOCAL archive at > > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > I was interested to image a round spiky object in 3-D and I know hoheria > > pollen grain provides this shape. > > If any one knows any other specimen ... > > > > On Fri, Jun 13, 2008 at 10:39 PM, Christian <[hidden email]> wrote: > > > >> Search the CONFOCAL archive at > >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >> > >> > https://www.triarchmicroslides.com/search.aspx?keyword=pollen&Search=Search > >> > >> You might contact them directly as they very often have materials not on > >> the site. The question I have is why that species of pollen?!? > >> > >> > >> *Julio Vazquez <[hidden email]>* wrote: > >> > >> Search the CONFOCAL archive at > >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal = > >> These three sites sell prepared biology slides, including ones with > >> pollens... don't know if any of them contains your Hoheria: > >> > >> http://www.discoverthis.com/slides-pollen.html > >> > >> http://www.hometrainingtools.com/ > >> > >> http://www.carolina.com > >> > >> -- > >> Julio Vazquez > >> Fred Hutchinson Cancer Research Center > >> Seattle, WA 98109-1024 > >> > >> http://www.fhcrc.org/ > >> > >> On Jun 13, 2008, at 8:11 AM, Sarah Kefayati wrote: > >> > >> Search the CONFOCAL archive at > >> http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Dear list > >> > >> we had a correction for our diode laser beam and I would like to test the > >> image with some pollen grain,and specially Hoheria sp pollen grain. > >> Does anyone know any company that has the prepared hoheria pollen grain > >> slide? > >> > >> I appreciate any information or suggestion. > >> > >> Regards > >> Sarah > >> > >> > >> > >> > >> > >> > > > > > -- > Chris Tully > Microscopy and Image Analysis Expert > [hidden email] > 240-888-1021 > http://www.linkedin.com/in/christully > |
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This is interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence?
Cheers
Shalin
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I think it's phenolics that are responsible for the
fluorescence. It
is pretty broad but you can see different peaks (depending
on the
pollen).
I had thought that spiky pollen grains would be a good TIRF
test
sample but not so - the fluorescence is deep enough below
the
surface to be out of TIRF range.
Guy
Optical Imaging Techniques in Cell Biology From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta Sent: Tuesday, 17 June 2008 10:08 AM To: [hidden email] Subject: Re: Pollen grain
This is interesting, do pollen's have nice excitation-emission
properties? Do they have specific peaks or just broad excitation and emission?
What would be the underlying biological organelle/molecule responsible for
autofluorescence?
Cheers
Shalin
No virus found in this incoming message. No virus found in this outgoing message. |
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In reply to this post by Shalin Mehta
Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Different pollen have different spectra. -mc > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > >> >> >> I also keep one of the pollen grain slides in the confocal room. When a >> user >> says that there's something wrong with the scope, I ask them if they've >> checked >> will the pollen slide. Since they inevitably haven't, I tell them to do >> that >> first, and then come back if there's a problem with the microscope. >> Amazingly, >> they rarely return. >> > > > This is interesting, do pollen's have nice excitation-emission > properties? > Do they have specific peaks or just broad excitation and emission? What > would be the underlying biological organelle/molecule responsible for > autofluorescence? > > Cheers > Shalin > >> >> Kristi DeCourcy >> > >> >> >> >> -- >> ~~~~~~~~~~~~~~~~~~~~~~~~~ >> Shalin Mehta >> mobile: +65-90694182 >> blog: shalin.wordpress.com >> ~~~~~~~~~~~~~~~~~~~~~~~~~~ >> Bioimaging Lab, Block-E3A, #7-10 >> Div of Bioengineering, NUS Singapore 117574 >> website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html >> >> Liver Cancer Functional Genomics Lab, #6-05 >> National Cancer Centre, Singapore 169610 >> http://www.nccs.com.sg/researcher/02_04d.htm >> > _________________________________________ Michael Cammer http://www.aecom.yu.edu/aif/ |
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In reply to this post by Guy Cox
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Mr. Blaudow and Mr. Norman were married in San Francisco this evening. Judy Cole was the best man, ring bearer, and maid of honor (she has video of the occasion). I know you wish the couple well. Dr. Vance broke down during the visit from Dr. Bayer. He will see her again soon about her future and about teaching the class in the Fall soon. Dr. Meyer and the Dean have given the OK to Dr. Bayer (pending the gracious blessing of the provost) to search for the new Director of the IMC this Fall. The position will be 75% in the Biology department and the rest in the IMC. We will look for a Cell or Molecular Biologist. Can I recommend you to serve on the search committee? It is very hot here. We are looking forward to the river rising (but not too much) from the midwestern floods. Hope you are enjoying your summer, Lewis On 6/16/08 8:44 PM, "Guy Cox" <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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In reply to this post by Guy Cox
Search the CONFOCAL archive at
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Mr. Blaudow and Mr. Norman were married in San Francisco this evening. Judy Cole was the best man, ring bearer, and maid of honor (she has video of the occasion). I know you wish the couple well. We will have a post wedding party (with wedding cake) at my house when they get back. Lewis On 6/16/08 8:44 PM, "Guy Cox" <[hidden email]> wrote: Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal |
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Hello Group,
I am not sure if this is a simple mistake or a joke, but perhaps Dr. Coons should be made aware that his personal emails are being directed to microscopy folks around the world. If this is meant to be a gag, my apologies. Farid On Mon, Jun 16, 2008 at 10:12 PM, Lewis B Coons (lcoons) <[hidden email]> wrote:
-- Farid Jalali MSc Senior Research Technician- Lab Manager Applied Molecular Oncology/ Princess Margaret Hospital STTARR Innovation Facility/ Radiation Medicine Program Toronto, Canada 416-946-4501 X4351 (Princess Margaret Hospital) 416-581-7754 STTARR at MaRS Building 416-581-7791 STTARR Microscopy Suite |
 
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Jeremy Adler |
Re: Pollen gr=?iso-8859-1?Q?=E0in_?= RI ?
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http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal Does anyone have a plausible RI for pollen grains, or the RI of a medium that produces the best images ? Jeremy Adler Cell Biology The Wenner-Gren Inst. Arrhenius Laboratories E5 Stockholm University Stockholm 106 91 Sweden ________________________________ From: Confocal Microscopy List on behalf of Guy Cox Sent: Tue 6/17/2008 03:44 To: [hidden email] Subject: Re: Pollen grain Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I think it's phenolics that are responsible for the fluorescence. It is pretty broad but you can see different peaks (depending on the pollen). I had thought that spiky pollen grains would be a good TIRF test sample but not so - the fluorescence is deep enough below the surface to be out of TIRF range. Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net <http://www.guycox.net/> ________________________________ From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta Sent: Tuesday, 17 June 2008 10:08 AM To: [hidden email] Subject: Re: Pollen grain Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal I also keep one of the pollen grain slides in the confocal room. When a user says that there's something wrong with the scope, I ask them if they've checked will the pollen slide. Since they inevitably haven't, I tell them to do that first, and then come back if there's a problem with the microscope. Amazingly, they rarely return. This is interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence? Cheers Shalin Kristi DeCourcy -- ~~~~~~~~~~~~~~~~~~~~~~~~~ Shalin Mehta mobile: +65-90694182 blog: shalin.wordpress.com <http://shalin.wordpress.com/> ~~~~~~~~~~~~~~~~~~~~~~~~~~ Bioimaging Lab, Block-E3A, #7-10 Div of Bioengineering, NUS Singapore 117574 website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html Liver Cancer Functional Genomics Lab, #6-05 National Cancer Centre, Singapore 169610 http://www.nccs.com.sg/researcher/02_04d.htm No virus found in this incoming message. Checked by AVG. Version: 7.5.524 / Virus Database: 270.3.0/1505 - Release Date: 16/06/2008 7:20 AM No virus found in this outgoing message. Checked by AVG. Version: 7.5.524 / Virus Database: 270.3.0/1505 - Release Date: 16/06/2008 7:20 AM |
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Peter Zoon |
Re: Pollen gr=?ISO-8859-1?Q?=E0in_?= RI ?
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Search the CONFOCAL archive at
http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal In general I'd say the best images are produced if the refractive medium is the same as that of your objective immersion medium (oil with oil and water with water). Jeremy Adler wrote: > Search the CONFOCAL archive at > http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > Does anyone have a plausible RI for pollen grains, or the RI of a medium that produces the best images ? > > Jeremy Adler > Cell Biology > The Wenner-Gren Inst. > Arrhenius Laboratories E5 > Stockholm University > Stockholm 106 91 > Sweden > > ________________________________ > > From: Confocal Microscopy List on behalf of Guy Cox > Sent: Tue 6/17/2008 03:44 > To: [hidden email] > Subject: Re: Pollen grain > > > Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > I think it's phenolics that are responsible for the fluorescence. It > is pretty broad but you can see different peaks (depending on the > pollen). > > I had thought that spiky pollen grains would be a good TIRF test > sample but not so - the fluorescence is deep enough below the > surface to be out of TIRF range. > > Guy > > > > Optical Imaging Techniques in Cell Biology > by Guy Cox CRC Press / Taylor & Francis > http://www.guycox.com/optical.htm > ______________________________________________ > Associate Professor Guy Cox, MA, DPhil(Oxon) > Electron Microscope Unit, Madsen Building F09, > University of Sydney, NSW 2006 > ______________________________________________ > Phone +61 2 9351 3176 Fax +61 2 9351 7682 > Mobile 0413 281 861 > ______________________________________________ > http://www.guycox.net <http://www.guycox.net/> > > > > ________________________________ > > From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Shalin Mehta > Sent: Tuesday, 17 June 2008 10:08 AM > To: [hidden email] > Subject: Re: Pollen grain > > > Search the CONFOCAL archive at http://listserv.acsu.buffalo.edu/cgi-bin/wa?S1=confocal > > > I also keep one of the pollen grain slides in the confocal room. When a user > says that there's something wrong with the scope, I ask them if they've checked > will the pollen slide. Since they inevitably haven't, I tell them to do that > first, and then come back if there's a problem with the microscope. Amazingly, > they rarely return. > > > > > This is interesting, do pollen's have nice excitation-emission properties? Do they have specific peaks or just broad excitation and emission? What would be the underlying biological organelle/molecule responsible for autofluorescence? > > Cheers > Shalin > > > Kristi DeCourcy > > > > > > > -- > ~~~~~~~~~~~~~~~~~~~~~~~~~ > Shalin Mehta > mobile: +65-90694182 > blog: shalin.wordpress.com <http://shalin.wordpress.com/> > ~~~~~~~~~~~~~~~~~~~~~~~~~~ > Bioimaging Lab, Block-E3A, #7-10 > Div of Bioengineering, NUS Singapore 117574 > website: http://www.bioeng.nus.edu.sg/optbioimaging/colin/index.html > > Liver Cancer Functional Genomics Lab, #6-05 > National Cancer Centre, Singapore 169610 > http://www.nccs.com.sg/researcher/02_04d.htm > > > No virus found in this incoming message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 270.3.0/1505 - Release Date: 16/06/2008 7:20 AM > > > > No virus found in this outgoing message. > Checked by AVG. > Version: 7.5.524 / Virus Database: 270.3.0/1505 - Release Date: 16/06/2008 7:20 AM > > -- Peter D. Zoon Centre for Advanced Microscopy Section of Molecular Cytology Swammerdam Institute for Life Sciences Faculty of Science, University of Amsterdam Visit.mail: Kruislaan 316 (room 2.03) 1098 SM Amsterdam The Netherlands E-mail: [hidden email] Tel: +31-(0)20-5257860 Web: http://wwwmc.bio.uva.nl/ http://www.science.uva.nl/sils http://z00n.net/ |
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