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Applications are invited for a Postdoctoral position in the London Centre
for Nanotechnology (LCN), in the area of electrophysiology and cell
biophysics. The aim of the project is to study pressure propagation within
living cells using patch clamp electrophysiology, molecular biology and
confocal microscopy.
One of the most striking features of eukaryotic cells is their capacity to
change shape in response to environmental or intrinsic cues thanks to their
actomyosin cytoskeleton. In studies of cellular morphogenesis, the cytoplasm
is generally viewed as an innocuous backdrop enabling diffusion of
signalling proteins. This view misses a major point: the time-dependent
mechanical properties of cells are mainly determined by their cytoplasm
because it forms the largest part of the cell by volume. We have previously
shown that the cytoplasm is akin a fluid-filled sponge and the aim of this
project will be to further explore and characterise the physical properties
of cytoplasm. One goal of this project will be to directly ask if pressure
gradients exist within cells. First, we will use whole-cell patch clamp to
break open the cell and ask if this perturbs the extension of
pressure-driven protrusions (blebs). Second, we will impose an intracellular
pressure transient via a patch pipette and image pressure diffusion using
fluorescence defocusing microscopy of fluorescent beads tethered to the cell
surface. Another aim will be to map the spatial distribution of pore sizes
throughout the cell using changes in quantum dot mobility in response to
dehydration. The candidate will have the opportunity to work collaboratively
with colleagues within LCN and UCL.
The successful candidate will have a PhD in a relevant area of biophysics,
or cell and molecular biology. *A strong background in electrophysiology,
molecular biology, and confocal microscopy is desired. *Additional
experience in cell biophysics, finite element modelling, and programming
will be an advantage. The appointment will be on UCL Grade 7. The salary
range will be £31,778 £38,441 per annum, inclusive of London Allowance.
The position will initially be for two years. Informal inquiries may be made
directly to Dr Guillaume Charras, who will be supervising the research
(
[hidden email],
http://www.london-nano.com/our-people/academics/guillaume-charras).
For further details about the vacancy and how to apply on line please go to
http://www.ucl.ac.uk/hr/jobs/ and search on Reference Number 1171994.
Taking Action for Equality
The closing date for applications is 1st March 2011.
Locked
