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Practical question (cry for help) about ZEISS LSM 510

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Michelle Aloni

Practical question (cry for help) about ZEISS LSM 510

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Hi list,

We have a ZEISS LSM 510 confocal microscope in our busy core.

Unfortunately, immersion oil got into our 40X oil and 63X oil lenses and maybe even in the 20X air. I was told that they should be send to Germany for repair. This would be fine, but no one knows how long it will take before we can get them back...

ZEISS guys in Los Angeles told me that they don't have lenses to loan...

Our schedule for the users is full every day (even on weekends). We can't close the core!

What do you guys do when something like this happen? There is no way that we are the first facility facing this problem.

After we got in trouble, we learned our lesson the hard way and everybody is now extremely careful, plus we purchased and are using the special collar which protects the lenses of the inverted microscope... One way or another the damage is already done and we need help.

Is there someone out there that might be able to loan us a lens or two for AXIO Observer Z1 for the duration of the cleaning/repair of our lenses?

If you want to, you can contact me off the list at [hidden email]

Your advice or help would be highly appreciated

Michelle Aloni MS, HTL (ASCP)
Research Specialist
USC Keck School of Medicine LA, CA

Craig Brideau

Re: Practical question (cry for help) about ZEISS LSM 510

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Franky, most microscope companies take a very long time to do repairs. In
our lab we learned our lesson a while ago. When some grant money came up we
purchased a duplicate of our most critical lens and kept it in safe
storage. When the lens on the microscope was inevitably broken we were able
to swap in the back-up lens while waiting for the damaged one to be
repaired. In my experience it takes about 6-8 weeks for a repair to go
through. Have you other listers seen better or worse times?

Craig

On Mon, Oct 17, 2011 at 6:54 PM, Michelle MacVeigh-Aloni
<[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi list,
>
> We have a ZEISS LSM 510 confocal microscope in our busy core.
>
> Unfortunately, immersion oil got into our 40X oil and 63X oil lenses and
> maybe even in the 20X air. I was told that they should be send to Germany
> for repair. This would be fine, but no one knows how long it will take
> before we can get them back...
>
> ZEISS guys in Los Angeles told me that they don't have lenses to loan...
>
> Our schedule for the users is full every day (even on weekends). We can't
> close the core!
>
> What do you guys do when something like this happen? There is no way that
> we are the first facility facing this problem.
>
> After we got in trouble, we learned our lesson the hard way and everybody
> is now extremely careful, plus we purchased and are using the special collar
> which protects the lenses of the inverted microscope... One way or another
> the damage is already done and we need help.
>
> Is there someone out there that might be able to loan us a lens or two for
> AXIO Observer Z1 for the duration of the cleaning/repair of our lenses?
>
> If you want to, you can contact me off the list at [hidden email]
>
> Your advice or help would be highly appreciated
>
> Michelle Aloni MS, HTL (ASCP)
> Research Specialist
> USC Keck School of Medicine LA, CA
>

Elena Rebollo Arredondo

Re: Practical question (cry for help) about ZEISS LSM 510

In reply to this post by Michelle Aloni

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Hi,

having oil into the 40x, 63x and 20x at the same time is statistically very
unlikely. No joke, I have some really absent minded users and it takes many
atemps to really get oil into the lenses. I would recommend having a
thorough look under the stereomicroscope plus a good cleaning protocol,
sometimes the usual optical paper is not enough.

If it is true, then panic. In my experience (with Leica), the usual time is
six weeks...

Cheers,

E

On 18 October 2011 02:54, Michelle MacVeigh-Aloni <[hidden email]> wrote:

--
Elena Rebollo Ph.D.
Confocal Microscopy Unit IBMB-PCB
Molecular Biology Institute of Barcelona (IBMB), CSIC
c/ Baldiri Reixac,10-12
08028 Barcelona (Spain)
Tel (+34) 620938111/ (+34) 934020249
www.ibmb.csic.es<http://www.ibmb.csic.es/index.php?pg=laboratorio&idLaboratorio=27&tab=lab_home>

Julien Cau

Re: Practical question (cry for help) about ZEISS LSM 510

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Hi,

A while ago, we had such a pandemics (few objectives with the front lens
leaking in a few weeks time).
The likely reason for this was a mistake in the objective cleaning
solution we were preparing. Costly error....
You can monitor this under a stereomicroscope, if you see
discontinuities in the glue seal sticking the front lens in several
fairly new objectives, it's time to change your objective cleaning protocol.
Regarding your local representative, I can't believe they won't offer
you a temporary solution.
Best regards

Le 18/10/2011 19:53, Elena Rebollo Arredondo a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> having oil into the 40x, 63x and 20x at the same time is statistically very
> unlikely. No joke, I have some really absent minded users and it takes many
> atemps to really get oil into the lenses. I would recommend having a
> thorough look under the stereomicroscope plus a good cleaning protocol,
> sometimes the usual optical paper is not enough.
>
> If it is true, then panic. In my experience (with Leica), the usual time is
> six weeks...
>
> Cheers,
>
> E
>
>
> On 18 October 2011 02:54, Michelle MacVeigh-Aloni<[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi list,
>>
>> We have a ZEISS LSM 510 confocal microscope in our busy core.
>>
>> Unfortunately, immersion oil got into our 40X oil and 63X oil lenses and
>> maybe even in the 20X air. I was told that they should be send to Germany
>> for repair. This would be fine, but no one knows how long it will take
>> before we can get them back...
>>
>> ZEISS guys in Los Angeles told me that they don't have lenses to loan...
>>
>> Our schedule for the users is full every day (even on weekends). We can't
>> close the core!
>>
>> What do you guys do when something like this happen? There is no way that
>> we are the first facility facing this problem.
>>
>> After we got in trouble, we learned our lesson the hard way and everybody
>> is now extremely careful, plus we purchased and are using the special collar
>> which protects the lenses of the inverted microscope... One way or another
>> the damage is already done and we need help.
>>
>> Is there someone out there that might be able to loan us a lens or two for
>> AXIO Observer Z1 for the duration of the cleaning/repair of our lenses?
>>
>> If you want to, you can contact me off the list at [hidden email]
>>
>> Your advice or help would be highly appreciated
>>
>> Michelle Aloni MS, HTL (ASCP)
>> Research Specialist
>> USC Keck School of Medicine LA, CA
>>
>
>

--

____________________________________________

*/Julien Cau, PhD./*

/Montpellier RIO Imaging Facility manager/Responsable technique MRI/

Montpellier RIO Imaging

Montpellier BIOCAMPUS, UMS3426

Arnaud de Villeneuve Campus Imaging Facility

Institut de Génétique Humaine-CNRS

141, rue de la Cardonille

F-34396 Montpellier(France)

e-mail: [hidden email].fr_ <mailto:[hidden email]>

phone: +33.4.34.35.99.90

mobile: +33.6.50.19.27.49

fax: +33.4.34.35.99.01

URL: _http://www.mri.cnrs.fr/_

____________________________________________

Armstrong, Brian

Re: Practical question (cry for help) about ZEISS LSM 510

In reply to this post by Michelle Aloni

Michelle, it is possible that I could loan you some Zeiss objectives.
Contact me if you still need help.

Brian Armstrong PhD
Assistant Research Professor
Light Microscopy Core
Beckman Research Institute
City of Hope
1500 East Duarte Road
Duarte, CA 91010
626-256-4673 x62872

Light Microscopy Core Facility

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Michelle MacVeigh-Aloni
Sent: Monday, October 17, 2011 5:55 PM
To: [hidden email]
Subject: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi list,

We have a ZEISS LSM 510 confocal microscope in our busy core.

Unfortunately, immersion oil got into our 40X oil and 63X oil lenses and maybe even in the 20X air. I was told that they should be send to Germany for repair. This would be fine, but no one knows how long it will take before we can get them back...

ZEISS guys in Los Angeles told me that they don't have lenses to loan...

Our schedule for the users is full every day (even on weekends). We can't close the core!

What do you guys do when something like this happen? There is no way that we are the first facility facing this problem.

After we got in trouble, we learned our lesson the hard way and everybody is now extremely careful, plus we purchased and are using the special collar which protects the lenses of the inverted microscope... One way or another the damage is already done and we need help.

Is there someone out there that might be able to loan us a lens or two for AXIO Observer Z1 for the duration of the cleaning/repair of our lenses?

If you want to, you can contact me off the list at [hidden email]

Your advice or help would be highly appreciated

Michelle Aloni MS, HTL (ASCP)
Research Specialist
USC Keck School of Medicine LA, CA

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Guy Cox-2

Re: Practical question (cry for help) about ZEISS LSM 510

In reply to this post by Craig Brideau

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I agree about repair times. These 'super lenses' are pretty expensive
but if you have standard 100x oil and 40x dry with your system (booth
typically moderate in price) you will have quite adequate objectives to
tide you over during repair times. Most users have neither the skill
nor the specimens to benefit from very expensive objectives anyway!

Guy

Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm
______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Australian Centre for Microscopy & Microanalysis,
Madsen Building F09, University of Sydney, NSW 2006

Phone +61 2 9351 3176 Fax +61 2 9351 7682
Mobile 0413 281 861
______________________________________________
http://www.guycox.net

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Craig Brideau
Sent: Wednesday, 19 October 2011 4:17 AM
To: [hidden email]
Subject: Re: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Franky, most microscope companies take a very long time to do repairs.
In
our lab we learned our lesson a while ago. When some grant money came
up we
purchased a duplicate of our most critical lens and kept it in safe
storage. When the lens on the microscope was inevitably broken we were
able
to swap in the back-up lens while waiting for the damaged one to be
repaired. In my experience it takes about 6-8 weeks for a repair to go
through. Have you other listers seen better or worse times?

Craig

On Mon, Oct 17, 2011 at 6:54 PM, Michelle MacVeigh-Aloni
<[hidden email]>wrote:

and
> maybe even in the 20X air. I was told that they should be send to
Germany
> for repair. This would be fine, but no one knows how long it will take
> before we can get them back...
>
> ZEISS guys in Los Angeles told me that they don't have lenses to
loan...
>
> Our schedule for the users is full every day (even on weekends). We
can't
> close the core!
>
> What do you guys do when something like this happen? There is no way
that
> we are the first facility facing this problem.
>
> After we got in trouble, we learned our lesson the hard way and
everybody
> is now extremely careful, plus we purchased and are using the special
collar
> which protects the lenses of the inverted microscope... One way or
another
> the damage is already done and we need help.
>
> Is there someone out there that might be able to loan us a lens or two
for
> AXIO Observer Z1 for the duration of the cleaning/repair of our
lenses?
>
> If you want to, you can contact me off the list at [hidden email]
>
> Your advice or help would be highly appreciated
>
> Michelle Aloni MS, HTL (ASCP)
> Research Specialist
> USC Keck School of Medicine LA, CA
>

-----
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mcammer

Re: Practical question (cry for help) about ZEISS LSM 510

In reply to this post by Craig Brideau

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

A one-time insurance investment we made in a previous lab was purchasing a spare favorite objective, in this case an Olympus 60X N.A. 1.4 which worked great on both our Olympus and Zeiss stands. The up front cost may be great, but over a few years, especially in a fee generating facility, it pays off. (Just my $6k.)
-Michael C.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Tuesday, October 18, 2011 1:17 PM
To: [hidden email]
Subject: Re: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Franky, most microscope companies take a very long time to do repairs. In
our lab we learned our lesson a while ago. When some grant money came up we
purchased a duplicate of our most critical lens and kept it in safe
storage. When the lens on the microscope was inevitably broken we were able
to swap in the back-up lens while waiting for the damaged one to be
repaired. In my experience it takes about 6-8 weeks for a repair to go
through. Have you other listers seen better or worse times?

Craig

On Mon, Oct 17, 2011 at 6:54 PM, Michelle MacVeigh-Aloni
<[hidden email]>wrote:

------------------------------------------------------------
This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain information that is proprietary, confidential, and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, or distribution is prohibited. If you have received this email in error please notify the sender by return email and delete the original message. Please note, the recipient should check this email and any attachments for the presence of viruses. The organization accepts no liability for any damage caused by any virus transmitted by this email.
=================================

Christine Davis

Re: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I agree it can take 2-3 months to get an objective back. I'd look at it
under the stereo scope and try cleaning it. Also you can wrap the objective
in lens paper, put it in it's case, and turning it upside down (atleast
overnight). This might help leach some oil out. If oil has gotten inside
on the lens then it wont cure your problem. If oil has loaded itself on/in
the shelf below the lens it will help. Maybe you can get a good enough
image on one or two lenses to give yourself time to get one properly fixed.

Christie Davis

On Wed, Oct 19, 2011 at 11:28 AM, Cammer, Michael <
[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> A one-time insurance investment we made in a previous lab was purchasing a
> spare favorite objective, in this case an Olympus 60X N.A. 1.4 which worked
> great on both our Olympus and Zeiss stands. The up front cost may be great,
> but over a few years, especially in a fee generating facility, it pays off.
> (Just my $6k.)
> -Michael C.
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Craig Brideau
> Sent: Tuesday, October 18, 2011 1:17 PM
> To: [hidden email]
> Subject: Re: Practical question (cry for help) about ZEISS LSM 510
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Franky, most microscope companies take a very long time to do repairs. In
> our lab we learned our lesson a while ago. When some grant money came up
> we
> purchased a duplicate of our most critical lens and kept it in safe
> storage. When the lens on the microscope was inevitably broken we were
> able
> to swap in the back-up lens while waiting for the damaged one to be
> repaired. In my experience it takes about 6-8 weeks for a repair to go
> through. Have you other listers seen better or worse times?
>
> Craig
>
>
> On Mon, Oct 17, 2011 at 6:54 PM, Michelle MacVeigh-Aloni
> <[hidden email]>wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi list,
> >
> > We have a ZEISS LSM 510 confocal microscope in our busy core.
> >
> > Unfortunately, immersion oil got into our 40X oil and 63X oil lenses and
> > maybe even in the 20X air. I was told that they should be send to
> Germany
> > for repair. This would be fine, but no one knows how long it will take
> > before we can get them back...
> >
> > ZEISS guys in Los Angeles told me that they don't have lenses to loan...
> >
> > Our schedule for the users is full every day (even on weekends). We
> can't
> > close the core!
> >
> > What do you guys do when something like this happen? There is no way that
> > we are the first facility facing this problem.
> >
> > After we got in trouble, we learned our lesson the hard way and everybody
> > is now extremely careful, plus we purchased and are using the special
> collar
> > which protects the lenses of the inverted microscope... One way or
> another
> > the damage is already done and we need help.
> >
> > Is there someone out there that might be able to loan us a lens or two
> for
> > AXIO Observer Z1 for the duration of the cleaning/repair of our lenses?
> >
> > If you want to, you can contact me off the list at [hidden email]
> >
> > Your advice or help would be highly appreciated
> >
> > Michelle Aloni MS, HTL (ASCP)
> > Research Specialist
> > USC Keck School of Medicine LA, CA
> >
>
> ------------------------------------------------------------
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The organization
> accepts no liability for any damage caused by any virus transmitted by this
> email.
> =================================
>

Jacqueline Ross

Re: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I recommend trying the polystyrene method suggested recently by Guy Cox. It worked brilliantly for one of our dry objective lenses that had somehow got coated in something. You do need to do it under a stereo as suggested by Christine.

Kind regards,

Jacqui

Jacqueline Ross

Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland, NEW ZEALAND

Tel: 64 9 373 7599 Ext 87438
Fax: 64 9 373 7484

http://www.fmhs.auckland.ac.nz/sms/biru/

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Christine Davis
Sent: Friday, 21 October 2011 7:33 a.m.
To: [hidden email]
Subject: Re: Practical question (cry for help) about ZEISS LSM 510

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

I agree it can take 2-3 months to get an objective back. I'd look at it
under the stereo scope and try cleaning it. Also you can wrap the objective
in lens paper, put it in it's case, and turning it upside down (atleast
overnight). This might help leach some oil out. If oil has gotten inside
on the lens then it wont cure your problem. If oil has loaded itself on/in
the shelf below the lens it will help. Maybe you can get a good enough
image on one or two lenses to give yourself time to get one properly fixed.

Christie Davis

On Wed, Oct 19, 2011 at 11:28 AM, Cammer, Michael <
[hidden email]> wrote:

Ben Abrams

Re: Practical question (cry for help) about ZEISS LSM 510

This post was updated on .

CONTENTS DELETED

The author has deleted this message.

lechristophe

Re: Practical question (cry for help) about ZEISS LSM 510

*****
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Mr Abrams, would it be possible to turn off that autoreply thing or avoid
it next time ? Please be aware that it seems to send a reply to everyone on
the Confocal Mailing list, each time there is a message from anyone on the
list.

Thanks a lot,

Christophe Leterrier

On Fri, Oct 21, 2011 at 00:29, Ben Abrams <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> This is an automated reply.
>
> I will be out of the office/ attending a course and will have uncertain
> email access Monday October 10 through Friday October 21.
>