Pseudo DAPI on an old 510

[Ki Hng]

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Hello all,

The harddrive on our old Zeiss 510 failed recently and was replaced.  We
lost most of the configurations on it, but it has now been restored to
(mostly) fully working condition.

It doesn't have a laser for DAPI, however in the past it was configured for
pseudo-DAPI, ie - using the epifluorescence light source to image in the
DAPI channel.  

Now after the repair that doesn't work.  I can get it to work while in 'Fast
XY' by manually opening the shutter and selecting the correct filter cube
mid-live-scan.  However once I stop the live scan, and click on 'Single' or
'Start', it closes the fluorescence shutter and puts a blank into the
filtercube position.  Saving the light path doesn't work, and I've tried
everything I could think of.  

If anyone has the method to set it up for Pseudo DAPI imaging, I would be
grateful.  Thank you.  

Keng

[PEARSON Matthew]

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Hi Keng,

I suspect we have an older version of the 510 here but this is our  
single track config for pseudo DAPI:

Specimen>NT 80/20, Mirror, Mirror, BP 390-495, Ch2.

We use one of the sliding filter cube turret positions as the epi-
fluorescence shutter.  The pseudo DAPI setting just moves the slider  
to the DAPI position.

I can't really explain why its not saving the light path and  
microscope settings that you've selected, unless for some reason there  
are write issues to the folder where configs are saved to on the hard  
disk?  I only say that because you've just had it replaced..

Thanks,

Matt

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The University of Edinburgh is a charitable body, registered in
Scotland, with registration number SC005336.

[Zac Arrac Atelaz]

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Matt:
 
Reinstalling the program do that trick to you, now you are working in the "normal" way, the only thing you have to do, is change the filter cube for your dapi to the position where the system is making the confocal imaging, that might fix it.
 
Best regards and happiness
 
Gabriel OH
 

> Date: Wed, 24 Jul 2013 17:31:46 +0100
> From: [hidden email]
> Subject: Re: Pseudo DAPI on an old 510
> To: [hidden email]
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Keng,
>
> I suspect we have an older version of the 510 here but this is our  
> single track config for pseudo DAPI:
>
> Specimen>NT 80/20, Mirror, Mirror, BP 390-495, Ch2.
>
> We use one of the sliding filter cube turret positions as the epi-
> fluorescence shutter.  The pseudo DAPI setting just moves the slider  
> to the DAPI position.
>
> I can't really explain why its not saving the light path and  
> microscope settings that you've selected, unless for some reason there  
> are write issues to the folder where configs are saved to on the hard  
> disk?  I only say that because you've just had it replaced..
>
> Thanks,
>
> Matt
>
> --
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.