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It will matter greatly what you are imaging the light scattering of the
sample. I have added the DeepSee compensator to our Ti:Sa, MP setup for
imaging blood vessels in mice. I experienced significant increase in
brightness (3X) and mirror depth improvement (~10-15%). Hope that this
helps.
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax
Email <mailto:
[hidden email]>
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Website <
http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm
> Hi everybody,
> I am planning to image fluorescent neurons in vivo approximately 200
> um below the pia with a standard Ti:Sa laser.
> I wonder if there is a clear advantage to use pulse compression to
> compensate for dispersion of pulses due to tissue.
> I understand theoretical arguments in favor of pulse compression.
> However from an experimental point of view, are there some people here
> in the list that have experienced some gain (in lowering the intensity
> of the exciting beam for example).
> Any comments would be greatly appreciated.
> Thanks a lot
> Stephane
>
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