Quantification 3D reconstruction

[Carlos Lizama]

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Hi everybody,

I need to a quantification in a z-stack I dont know which is the best way to do it?
I was try to do it with image J in 3D reconstruction but my problems is when
imageJ and the other software generate the 3d reconstruction it make a average
with the fluorescence to generate the 3D reconstruction, And the other thing is I
dont know if in imagej is posible select an area in the photo a request the
quantiication in all the slides, Because doing the quantification in each slide is
really slow and complicate i think. mmm ImageJ has a special tools or plugin to
do the quantification? Because I was tried using the tools to select area and then
generate histogram or measure options.

Thanks
Carlos

[Bonnaud, Fabien]

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Dear Carlos,

Following-up with your previous post about Volocity concerning 3D image visualization, I also confirm you that Volocity provides a complete suite of tools to perform 3D analysis.

It allows 3D image segmentation, objects populations analysis, comparison of biological conditions, automatic 3D tracking, 3D co-localization analysis, ratio measurements, FRAP analysis, ...
The output can be numerical with analysis and re-analysis of the data, identification of patterns and trends in one or more images, chart edition and export, or visual with snapshots and movies of your images and measurements.

The analysis module of Volocity is called Volocity Quantitation.

More information about the analysis functions of Volocity can be found here :

http://www.perkinelmer.com/pages/020/cellularimaging/training/default.xhtml 

should you have any specific question or need any further assistance, please do not hesitate to contact me and / or our technical support desk :

http://cellularimaging.perkinelmer.com/support/ 

Sincerely yours,

Fabien Bonnaud | Volocity Product Line Leader, Life Sciences & Technology
PerkinElmer | For the Better                                                                  
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-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de Carlos Lizama
Envoyé : vendredi 6 juillet 2012 04:34
À : [hidden email]
Objet : Quantification 3D reconstruction

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi everybody,

I need to a quantification in a z-stack I dont know which is the best way to do it?
I was try to do it with image J in 3D reconstruction but my problems is when
imageJ and the other software generate the 3d reconstruction it make a average
with the fluorescence to generate the 3D reconstruction, And the other thing is I
dont know if in imagej is posible select an area in the photo a request the
quantiication in all the slides, Because doing the quantification in each slide is
really slow and complicate i think. mmm ImageJ has a special tools or plugin to
do the quantification? Because I was tried using the tools to select area and then
generate histogram or measure options.

Thanks
Carlos