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Quantitative Imaging: From Acquisition to Analysis
March 29 - April 11, 2017
Applications due January 20, 2017
More info & application:
https://meetings.cshl.edu/courses.aspx?course=C-QICM&year=17Course Description:
Combining careful image acquisition with rigorous computational analysis
allows extraction of quantitative data from light microscopy images that
is far more informative and reproducible than what can be seen by eye.
This course will focus on advanced quantitative fluorescence microscopy
techniques used for imaging a range of biological specimens, from
tissues to cells to single molecules. The course is designed for
quantitative cell and molecular biologists, biophysicists and bioengineers.
We provide a thorough treatment of the complete process of quantitative
imaging, from the photons emitted from the sample to the extraction of
biologically meaningful measurements from digital images. Material is
covered in lectures, discussion groups and hands-on quantitative
exercises using commercial microscopes and open-source image analysis tools.
Concepts covered include:
• Widefield fluorescence microscopy
• Laser scanning and spinning disk confocal microscopy
• CCD, EM-CCD & sCMOS cameras
• Total internal fluorescence microscopy (TIRF)
• Light sheet microscopy
• Super-resolution microscopy (structured illumination, STED &
localization microscopy)
• Imaging and analyzing ratiometric “biosensors” (including FRET)
• Fluorescent proteins and live sample imaging
• Image processing (filtering, de-noising, corrections, deconvolution)
• Image segmentation
• Quantitative shape and intensity measurements
• Object detection and tracking
• Designing and troubleshooting quantitative imaging experiments
• ...and more!
The course will also include a series of seminars from guest speakers
who apply the methods we will discuss:
Confirmed 2017 Seminar Speakers (more TBA)…
Sara Abrahamsson, Rockefeller University
Nathan Shaner, The Scintillon Institute
Suliana Manley, Ecole Polytechnique Fédérale de Lausanne
Charless Fowlkes, UC Irvine
More info and online application:
https://meetings.cshl.edu/courses.aspx?course=C-QICM&year=17
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