Question about Cell Labeling Dye

[yee z]

Dear listers,

 

One of our facility user wants to record a live cell vedio and has a problem with labeling the cells.

She wants to see two type of cells merging into each other and is more interested in seeing membrane fusion. She has one cell transfected with GFP and would like to have another cell in red. The whole process might be 4-6 hours or even longer.

 

Which dye is most suitable for this experiment?

 

Any suggestions would be highly appreciated.

 

Thanks,

 

Yi

[Mayandi Sivaguru]

Try CellVue on the non-gfp line at far red excitations.

http://www.mtarget.com/cellvue.html

Shiv


Dear listers,
 
One of our facility user wants to record a live cell vedio and has a problem with labeling the cells.
She wants to see two type of cells merging into each other and is more interested in seeing membrane fusion. She has one cell transfected with GFP and would like to have another cell in red. The whole process might be 4-6 hours or even longer.
 
Which dye is most suitable for this experiment?
 
Any suggestions would be highly appreciated.
 
Thanks,
 
Yi

At 10:59 AM 10/14/2008, you wrote:

[hidden email]

Mayandi Sivaguru, PhD, PhD
Microscopy Facility Manager
8, Institute for Genomic Biology
University of Illinois at Urbana-Champaign
1206 West Gregory Dr.
Urbana, IL 61801 USA

Office: 217.333.1214
Fax: [phone]
[hidden email]
http://core.igb.uiuc.edu