Question about a super-resolution technique
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lechristophe |
Question about a super-resolution technique
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Dear all,
I jsut heard of a technique to obtain a super-resolution image from a series of low-resolution shifted images : http://www.und.edu/instruct/young/toast/Super-Resolution.html See also here : http://scien.stanford.edu/class/psych221/projects/99/stever/report.html Basically, you extract details from sub-pixels shift in the image. My understanding is that it could be useful for microscopy, but only for undersampled images where the limiting factor is the pixel size, and not the diffraction. Is that right that you can't break the diffusion barrier with this method ? Christophe |
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G. Esteban Fernandez |
Re: Question about a super-resolution technique
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Christophe,
We have a Zeiss LSM 5 LIVE with a detector of fixed 512 pixels. To achieve true 1024x1024 or 1536x1536 it uses the shift technique you describe, shifting by 1/2 or 1/3 pixel size to get 1024 or 1536, respectively. It is true that this increases resolution when pixels are the limiting factor, it does not break the diffraction limit of resolution.
-Esteban
On Wed, Jul 29, 2009 at 4:34 AM, Christophe Leterrier <[hidden email]> wrote: Dear all, -- G. Esteban Fernandez, Ph.D. Associate Director Molecular Cytology Core Facility University of Missouri 120 Bond Life Sciences Center Columbia, MO 65211 http://www.biotech.missouri.edu/mcc/ 573-882-4895 573-884-9676 fax |
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Armstrong, Brian |
Re: Question about a super-resolution technique
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Hello, I believe that camera manufacturers
have used “pixel shifting” to obtain super high resolution from
CCDs for years. For example the Leica DFC500 was a pixel shift camera that
could utilize up to 36 shots to compose a single image (final resolution around
4k x 3k). Brian D Armstrong PhD Light Microscopy Core Manager Beckman Research Institute City of Dept of Neuroscience Duarte, CA 91010 626-256-4673 x62872 http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx From: Christophe, We have a Zeiss LSM 5 LIVE with a detector of fixed 512 pixels.
To achieve true 1024x1024 or 1536x1536 it uses the shift technique you
describe, shifting by 1/2 or 1/3 pixel size to get 1024 or 1536,
respectively. It is true that this increases resolution when pixels are
the limiting factor, it does not break the diffraction limit of resolution. -Esteban On Wed, Jul 29, 2009 at 4:34 AM, Christophe Leterrier <[hidden email]>
wrote: Dear all,
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Leoncio A. Vergara |
Re: Question about a super-resolution technique
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In reply to this post by lechristophe
I think the result of the pixel shifting cameras is equivalent to a smoothed
version of the image that would have been acquired with a CCD with the higher number of pixels... in the example that would be like applying a 6x6 kernel... I am right? Leoncio Vergara MD Technical Director Integrated Microscopy Core (IMC) |
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In reply to this post by G. Esteban Fernandez
Best Regards
Colin
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Thanks,
Adrienne Date: Thu, 30 Jul 2009 13:31:44 +0200 From: [hidden email] Subject: unsubscribe To: [hidden email] Best Regards
Colin
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From: Adrienne Rollie Date: Thu, 30 Jul 2009 10:12:36 -0700 To: <[hidden email]> Subject: unsubscribe Thanks, Adrienne Date: Thu, 30 Jul 2009 13:31:44 +0200 From: [hidden email] Subject: unsubscribe To: [hidden email] Best Regards Colin
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In reply to this post by Wright, Colin
Arrate Mallabiabarrena Unitat Microscòpia Òptica Avançada/ Advanced Light Microscopy Unit CRG-
Centre de Regulació Genòmica C/ Dr.
Aiguader, 88 08003
Barcelona Tel.
+34 93 316 01 95 Fax +
34 93 316 99 83 |
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In reply to this post by eric.f.eaton
Hi everyone,
Mailing list control addresses are listed in the headers of every message. The relevant parts are: -------------------- List-Help: <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY>, <mailto:[hidden email]?body=INFO%20CONFOCALMICROSCOPY> List-Unsubscribe: <mailto:[hidden email]> List-Subscribe: <mailto:[hidden email]> List-Owner: <mailto:[hidden email]> List-Archive: <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY> -------------------- Ian |
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What's up with all the defectors? ;-)
On Jul 31, 2009, at 10:42 AM, Ian Dobbie wrote: Hi everyone, Mailing list control addresses are listed in the headers of every message. The relevant parts are: -------------------- List-Help: <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY>, <mailto:[hidden email]?body=INFO% 20CONFOCALMICROSCOPY> List-Unsubscribe: <mailto:CONFOCALMICROSCOPY-unsubscribe- [hidden email]> List-Subscribe: <mailto:CONFOCALMICROSCOPY-subscribe- [hidden email]> List-Owner: <mailto:[hidden email]> List-Archive: <http://lists.umn.edu/cgi-bin/wa?LIST=CONFOCALMICROSCOPY> -------------------- Ian |
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> What's up with all the defectors? ;-) looks like the Olympus crewmen are leaving the ship!! : ^ ) > What's up with all the defectors? ;-) > > On Jul 31, 2009, at 10:42 AM, Ian Dobbie wrote: > > Hi everyone, > > Mailing list control addresses are listed in the headers of every > message. > > The relevant parts are: > -------------------- > <mailto:[hidden email]?body=INFO% > 20CONFOCALMICROSCOPY> > List-Unsubscribe: <mailto:CONFOCALMICROSCOPY-unsubscribe- > [hidden email]> > List-Subscribe: <mailto:CONFOCALMICROSCOPY-subscribe- > [hidden email]> > List-Owner: <mailto:[hidden email]> > List-Archive: <http://lists.umn.edu/cgi- > bin/wa?LIST=CONFOCALMICROSCOPY>-------------------- > > Ian > |
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In reply to this post by Wright, Colin
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Gregg Sobocinski |
Reply regarding unsubscribing
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In reply to this post by Eric Scarfone
I
suspect the defections are a response for those who are going to be “out
of office”. The policy for some listservers is that those who will be “out
of the office” should unsubscribe, then resubscribe when they return. The
address for this is different from list postings, so it can be performed
without human intervention. I don’t remember what the official policy is
for this list, but as Ian
Dobbie pointed out, the address for subscribing and unsubscribing IS DIFFERENT
from that for posting. Below
is a copy of the precise instructions I received at one point. Disclaimer:
I’m not the administrator, just passing along the info that I have. ~Gregg Gregg Sobocinski Microscope Imaging Specialist University of Michigan, MCDB Dept. Ann Arbor, Michigan USA SUBSCRIPTION Send an email to [hidden email]
(and NOT [hidden email])
and put the following into the body of the message: *subscribe confocalmicroscopy* (note that it
*isn't* "subscribe confocal") Alternatively, a subscriber can go to the website http://lists.umn.edu/ and follow the
directions on the web page. LEAVING Send an email to [hidden email]
(and NOT [hidden email])
and put the following into the body of the message: *signoff confocalmicroscopy* (note that it *isn't*
"signoff confocal") Alternatively, a subscriber can go to the website http://lists.umn.edu/ and follow the
directions on the web page. From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Eric
Scarfone > What's up with all the defectors? ;-) looks like the Olympus crewmen are leaving the ship!! : ^ ) |
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