R=?ISO-8859-1?Q?=E9f.?= : Re: DABCO-mounting medium

[Louis Villeneuve]

Thanks a lot Russ

Louis

		De :        Russell Spear <[hidden email]>@LISTSERV.BUFFALO.EDU         Envoyé par :        Confocal Microscopy List <[hidden email]>         Pour :        [hidden email]         cc :        (ccc : Louis Villeneuve/ICM/Reg06/SSSS)         Objet :        Re: DABCO-mounting medium
	2008-03-11 09:55 Veuillez répondre à Confocal Microscopy List

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Hi

I use DABO in a formulation of 1% DABCO in 85 - 90 %  glycerol, with 10
- 15 % PBS  if I use FITC I adjust the pH to 8.0 - 8.5 using indicator
strips, as FITC is noticably brighter at a higher pH.

Russ



Russell N. Spear
Sr. Research Specialist
Dept. of Plant Pathology
Univ. of Wisconsin
1630 Linden Dr.
Madison WI 53706

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>>> Louis Villeneuve <[hidden email]> 03/11/08 8:14 AM
>>>
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Hi everyone,

I am looking for a recipe for mounting media with DABCO diluted in
glycerol.  The one I have is DABCO, 0.4% (diluted in PBS) mix 1:4 in
glycerol.  Also, where I could find a database for finding the
refractive
index of several mouting medium/media?

Thanks

Louis Villeneuve

Louis Villeneuve
Research Associate - Confocal Microscopy
Heart Montreal Institute- Research Center
5000 East Belanger
Montreal (Qc), Canada
H1T 1C8

514-376-3330 ext 3511
514-376-1355 (Fax)

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