RE BioRad PMT

[Allan Kachelmeier]

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Hi,

We're trying to keep a BioRad 1024 alive. Recently we had the 568nm channel
go green (zero). Laser is fine. Switching the power supplies causes both
channels to go green. Haven't yet switched only the little boards on the
PMT as it requires a bit of small soldering. Looking for some solid advice
before getting into it too heavily. The green will go to gray, black, or
red with change in offset. Anyone have any relevant BioRad PMT experience
to share? Thanks.

Allan Kachelmeier
Manager, confocal microscopy core
Oregon Hearing Research Center
OHSU

[Guy Cox-2]

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My recollection, based on modifying one in José Feijó's lab, is that there is a spare channel in the electronics, ie it will take 4 detectors but only has 3.  We used it to add an additional pmt for transmission detection (the normal transmission detector on a 1024 is just a photodiode).  We had to get one of those 'little boards' (preamps) which at the time we were able to source through a Zeiss back-door.  So you may have another channel to try.  However, if switching green & red pmts made both channels blank you would seem to have two faults - one in the pmt or preamp, and one in the electronics.   Nuno may be able to offer further advice.  He may even have some spares, since I don't think the 1024 is in use any more.  

                                            Guy

Guy Cox, Honorary Associate Professor
School of Medical Sciences

Australian Centre for Microscopy and Microanalysis,
Madsen, F09, University of Sydney, NSW 2006

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Allan Kachelmeier
Sent: Wednesday, 4 December 2013 6:05 AM
To: [hidden email]
Subject: RE BioRad PMT

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Hi,

We're trying to keep a BioRad 1024 alive. Recently we had the 568nm channel go green (zero). Laser is fine. Switching the power supplies causes both channels to go green. Haven't yet switched only the little boards on the PMT as it requires a bit of small soldering. Looking for some solid advice before getting into it too heavily. The green will go to gray, black, or red with change in offset. Anyone have any relevant BioRad PMT experience to share? Thanks.

Allan Kachelmeier
Manager, confocal microscopy core
Oregon Hearing Research Center
OHSU

[Nuno Moreno]

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Hi,

I have some technical sketches. Most likely the preamps are dead. Contact me off list for more details.

Cheers Guy!

Nuno Moreno


On 04 Dec 2013, at 01:08, Guy Cox <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> My recollection, based on modifying one in José Feijó's lab, is that there is a spare channel in the electronics, ie it will take 4 detectors but only has 3.  We used it to add an additional pmt for transmission detection (the normal transmission detector on a 1024 is just a photodiode).  We had to get one of those 'little boards' (preamps) which at the time we were able to source through a Zeiss back-door.  So you may have another channel to try.  However, if switching green & red pmts made both channels blank you would seem to have two faults - one in the pmt or preamp, and one in the electronics.   Nuno may be able to offer further advice.  He may even have some spares, since I don't think the 1024 is in use any more.  
>
>                                            Guy
>
> Guy Cox, Honorary Associate Professor
> School of Medical Sciences
>
> Australian Centre for Microscopy and Microanalysis,
> Madsen, F09, University of Sydney, NSW 2006
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Allan Kachelmeier
> Sent: Wednesday, 4 December 2013 6:05 AM
> To: [hidden email]
> Subject: RE BioRad PMT
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi,
>
> We're trying to keep a BioRad 1024 alive. Recently we had the 568nm channel go green (zero). Laser is fine. Switching the power supplies causes both channels to go green. Haven't yet switched only the little boards on the PMT as it requires a bit of small soldering. Looking for some solid advice before getting into it too heavily. The green will go to gray, black, or red with change in offset. Anyone have any relevant BioRad PMT experience to share? Thanks.
>
> Allan Kachelmeier
> Manager, confocal microscopy core
> Oregon Hearing Research Center
> OHSU