Dear listers,
This question is about using PS-speck (Invitrogen cat# P7220) beads for the purpose other than they were designed for. I am interested in their refractive index. I have developed software for computing images under DIC of known specimens, and am considering these beads as test specimens. The goal is to compare computed images with experimental images.
I have tried polystyrene beads with known RI and small-size but they are hard to spot. I could spot PS-speck beads easily due to their fluorescence and then switch to DIC for imaging.
Answers on or off the list are much appreciated.
best
Shalin
mobile: +65-90694182
blog:
shalin.wordpress.comBioimaging Lab, Block-E3A, #7-10
Div of Bioengineering, NUS Singapore 117574
website:
http://www.bioeng.nus.edu.sg/optbioimaging/colin/people.asp#shalinm
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