Re: 2011 Northwest Regional Cytometry Meeting, March 24 - 26 at OHSU

[Allan Kachelmeier]

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To all confocalists and cytometrists,



The 2011 Northwest Regional Cytometry Meeting, “Cytometry data: mining,
modeling, and management”, will be held March 24 – 26 at OHSU. The meeting
will focus on what to do with collected multivariate data, including
discussions related to noise, inferring from small numbers, multi-site
comparisons, correlative visualization, and automated gating. Confirmed
speakers include J. Paul Robinson, Garry Nolan, Norman Dovichi, Manfred
Auer, Masanobu Yamamoto, Maria James, Ger van den Engh, Ryan Brinkman, Bob
Zigon, C. Bruce Bagwell, and Dave Novo. The NWRCM per se will begin at 3pm
on Thursday, March 24, following the FICCS computational cytometry meeting.
The Thursday session is followed by an evening reception dinner. The NWRCM
continues Friday and Saturday morning. Complimenting the plenaries is a full
array of vendor talks, panel discussion on relevant informatic developments,
and technical symposia. The full roster and schedule of events is due to be
posted soon. Online registration is at
www.ohsu.edu/ohrc<http://www.ohsu.edu/ohrc<http://www.ohsu.edu/ohrc%3chttp:/www.ohsu.edu/ohrc>>
.



In addition to the NWRCM per se, the meeting is an umbrella for a number of
other events. Already mentioned is the FICCS computational cytometry meeting
on the 24th. Contact Raphael Gottardo or Ryan Brinkman at
[hidden email]<mailto:[hidden email]<[hidden email]%3cmailto:[hidden email]>>
for more information on the FICCS meeting. Also FloCyte will be holding two
courses, “Data analysis” on the 24th, and a new one, “Standardization,
validation, characterization, and controls” on the 26th, both taught by Dr.
Tim Bushnell. A 10% discount is available if also registered for the NWRCM.
Contact Sue DeMaggio at [hidden email] for more information or
registration. Finally, a free, half-day cytometry workshop for middle and
high school teachers, including lecture and hands-on activities, will be
held on the 26th.



Mark your calendars. As always, the NWRCM will be busy, thought-provoking,
and useful. If you have any questions, please don’t hesitate to contact the
coordinator at [hidden email]<mailto:[hidden email]<[hidden email]%3cmailto:[hidden email]>>
. Thanks.



Allan Kachelmeier

2011 NWRCM coordinator



Manager, Confocal microscopy core

Oregon Hearing Research Center

OHSU

[leoncio vergara]

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Hi

We are working on a project involving imaging of cells transfected with double stranded RNA. We have successfully used electroporation of cells in suspension, however we need to look at early events without the morphological disruption induced by the method. I have searched for methods to do electroporation in adherent cells and have found a few examples in the literature plus a couple of commercial hits. However without experience I am lost. I would appreciate any suggestions. Ideally we would like to achieve homogeneous transfection of cells in the monolayer while minimizing damage and preserving viability. Also we want to do this safely (if not much to ask).

Thanks in advance

Leoncio

Leoncio A. Vergara MD
Assistant Director
Center for Biomedical Engineering (CBME)
And Technical Director
Optical Microscopy Core (OMC)
Assistant Professor
Microbiology and Immunology
University of Texas Medical Branch
409-750-2153 (cell)