Re: 3D printers used by microscopists

[Zbigniew Mikulski]

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Hi All,

Sorry for the late additions but hope you will find them interesting. Our two most successful projects are Freequenza and contributions to Pumpy project.

Freequenza
https://3dprint.nih.gov/discover/3DPX-012172
A histology aid for slide immunostaining. Improves staining consistency, reduces reagent use, and saves time. Works with Shandon Plastic Coverplates. For technical specifications, check out this link. https://www.tedpella.com/technote_html/36107_TN.pdf

Pumpy
https://github.com/HenriquesLab/NanoJ-Fluidics/wiki/Pumpy-3D-Printing-MMeyer
3D-Pumpy is an open-source device, composed of a 3D-Printed body, easily accessible LEGO-parts, electronics, and labware. It is designed to automate and simplify fluid exchange experiments in microscopy.

Both were designed and printed by Matthew Meyer from our core. If you need more details, please feel free to contact him at [hidden email] or interact with us at @LJIMicroCore on Twitter.

Best, Zbigniew

Zbigniew Mikulski, PhD 
Director, Microscopy Core Facility, Instructor | La Jolla Institute for Immunology | 9420 Athena Circle, La Jolla, CA 92037

[Sylvie Le Guyader]

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Hi there list people

Did I miss where we are supposed to upload the files? We are happy to contribute with files for Nikon systems but I got the impression that the repository location was not finalised yet. If it is please let me know.

Med vänlig hälsning / Best regards

Sylvie

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-------- Original Message --------
Subject: Re: 3D printers used by microscopists
From: Zbigniew Mikulski
To: [hidden email]
CC:

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Hi All,

Sorry for the late additions but hope you will find them interesting. Our two most successful projects are Freequenza and contributions to Pumpy project.

Freequenza
https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2F3dprint.nih.gov%2Fdiscover%2F3DPX-012172&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C92f74f376b3c460621b508d7916c2500%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637137767024371140&amp;sdata=Rj60hQeKpPOnetHAAfMdIgoIwgPI2zOOsr6v7qSw39Y%3D&amp;reserved=0
A histology aid for slide immunostaining. Improves staining consistency, reduces reagent use, and saves time. Works with Shandon Plastic Coverplates. For technical specifications, check out this link. https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.tedpella.com%2Ftechnote_html%2F36107_TN.pdf&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C92f74f376b3c460621b508d7916c2500%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637137767024371140&amp;sdata=HO0c%2F5Sv2iFm3ICLuP0Sq%2BQwDuteCdF4gtnFF5TlDmY%3D&amp;reserved=0

Pumpy
https://eur01.safelinks.protection.outlook.com/?url=https%3A%2F%2Fgithub.com%2FHenriquesLab%2FNanoJ-Fluidics%2Fwiki%2FPumpy-3D-Printing-MMeyer&amp;data=02%7C01%7Csylvie.le.guyader%40KI.SE%7C92f74f376b3c460621b508d7916c2500%7Cbff7eef1cf4b4f32be3da1dda043c05d%7C0%7C0%7C637137767024371140&amp;sdata=HEL8yJPgjHsJXv3Mvl0cdxCPJz0Ft0zLAnY9x48zQO8%3D&amp;reserved=0
3D-Pumpy is an open-source device, composed of a 3D-Printed body, easily accessible LEGO-parts, electronics, and labware. It is designed to automate and simplify fluid exchange experiments in microscopy.

Both were designed and printed by Matthew Meyer from our core. If you need more details, please feel free to contact him at [hidden email] or interact with us at @LJIMicroCore on Twitter.

Best, Zbigniew

Zbigniew Mikulski, PhD
Director, Microscopy Core Facility, Instructor | La Jolla Institute for Immunology | 9420 Athena Circle, La Jolla, CA 92037



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[Zbigniew Mikulski]

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*****

Hi Sylvie,

NIH Exchange https://3dprint.nih.gov/ seems to be a good place for it. I think there was someone contacting NIH staff to ask for a microscopy category on the Exchange. Thingiverse https://www.thingiverse.com/ is another alternative.

One more example of a cool 3D-printed project a little closer to your home Sylvie. Co-developed by our talented colleague Gustaf Christoffersson.

Turning Up the Heat: Local Temperature Control During in vivo Imaging of Immune Cells
https://www.frontiersin.org/articles/10.3389/fimmu.2019.02036/full

In this report, we describe a low-cost, multipurpose, and tissue-stabilizing in vivo imaging platform that enables sensing and regulation of local tissue temperature. The effect of tissue temperature on local blood flow and leukocyte migration is demonstrated in muscle and skin. Two different models of vacuum windows are described in this report, however, the design of the vacuum window can easily be adapted to fit different organs and tissues.

Best, Zbigniew