Re: Adherence of satellite cells to glass **tech support response**

[Roy Edward-2]

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Dear Melia
You might also consider CyGEL.
This live cell mountant will permit a gentle thermo-reversible immobilisation of your cells (live) and should further retain the 3-D structure since there is no adherence involved to create a "pseudopod" between cell and glass surface.

Cells are added to cooled CyGEL and deposited onto the slide where it will "set". Overlay the coverslip and re-chill to liquefy temporarily allowing the CyGEL to spread under the coverslip. Allow to warm above RT and it will re-gel.

Like this, cells should be good for 1.5-2h of imaging.

Standard formulation of CyGEL is PBS-based and a version ready for your own culture medium is also available - CyGEL Sustain.

Please contact me off-list for a more detailed discussion and reference lists, etc.

Kind regards,
Roy

Sent from my iPhone
Roy Edward
Biostatus Ltd.
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>
> ------------------------------
>
> Date:    Wed, 18 May 2016 13:57:21 +0300
> From:    Melia Paizi <[hidden email]>
> Subject: Adherence of satellite cells to glass
>
> *****
> Hi.
>
> We need advice on how to improve adherence of satellite cells in culture to
> optical glass.
> We have tried matrigel, matrigel+polylysin, gelatin.
>
> Thank you!
> Melia Paizi, PhD
> Bioimaging Center
> Biomedical Core Facility
> The Bruce Rappaport Faculty of Medicine
>
> Technion - Israel Institute of Technology
>
> POB 9649 MS 9, Bat-Galim, Haifa 3109601
> Israel
>
> Tel. [phone] <[phone]>Fax. [phone] <[phone]>
> [hidden email]
> bcf.technion.ac.il
> ------------------------------