> *****
> To join, leave or search the confocal microscopy listserv, go to:
>
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on
http://www.imgur.com and include the link in your posting.
> *****
>
> Hi Adam,
> In my experience, mechanical stability is another issue that you need to
> take into account. How are you planning to mount and adjust the
> dichorics/mirrors? When we built ours (which was years ago), we had to
> contend with whatever micro-optomechanical mounts we could get and they do
> drift with time. Things could have improved and you want to look for
> mounts/holders that have ultralow or zero drift.
>
>
>
> On Mon, Jul 31, 2017 at 1:32 PM, Michael Giacomelli <
[hidden email]> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > Post images on
http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> > Hi Adam,
> >
> > The main issue we have flatness, since even very flat ones will introduce
> > geometric distortion. Rather than use the high grade ones, we just
> > calibrate it out by imaging a grid target with white light and then using
> > imagej to compute the unwarp matrix. Takes less than 100 ms per
> megapixel
> > per core to unwarp two channels in matlab this way. Make sure you have
> > tip/tilt control somewhere for the dichroics as well, otherwise centering
> > the cameras is very painful.
> >
> > Christopher Rowlands, who I think you met, also had a clever design at
> SPIE
> > this year for 6 (or maybe 8?) channel detection that balanced distortion
> > between channels to minimize the total error. Not sure if you need
> > something like that, but you could run your design by him.
> >
> > Mike
> >
> > On Mon, Jul 31, 2017 at 4:11 PM, Adam Glaser <
[hidden email]>
> > wrote:
> >
> > > *****
> > > To join, leave or search the confocal microscopy listserv, go to:
> > >
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> > > Post images on
http://www.imgur.com and include the link in your
> > posting.
> > > *****
> > >
> > > Hello everyone,
> > >
> > > I was wondering if anyone could offer advice on building an in-house
> > image
> > > splitter for microscopy.
> > >
> > > We are hoping to build one to split a rectangular ROI vertically into
> 2-4
> > > colors. The design seems straightforward, similar to the schematic of
> > the
> > > Optosplit by Andor
http://www.andor.com/scientific-cameras/multi-> > > wavelength-imaging/optosplit-iii#prettyPhoto[pi_gal]/1/.
> > >
> > > Are there any non-obvious factors to consider when building? We will
> be
> > > using 2 identical tube lenses, the first to image an adjustable slit
> for
> > > creating the cropped rectangular ROI, the second to re-focus onto our
> > sCMOS
> > > camera, and super resolution imaging grade dichroics from Semrock.
> > >
> > > Thanks,
> > > Adam
> > >
> >
>
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