Re: CONFOCALMICROSCOPY Digest - 11 May 2009 to 12 May 2009 (#2009-97)
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Re: CONFOCALMICROSCOPY Digest - 11 May 2009 to 12 May 2009 (#2009-97)
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From: Confocal Microscopy List To: [hidden email] Sent: Wed May 13 01:03:57 2009 Subject: CONFOCALMICROSCOPY Digest - 11 May 2009 to 12 May 2009 (#2009-97)
I recently downloaded instruview. this makes use of the database found here http://turmac13.chem.columbia.edu/fretview/test.dal This can be imported and other fluorophores added Good luck with the search The Invitrogen spectra-viewer has
superseded the Bio-Rad spectra viewer after Zeiss killed off Bio-Rad [UK]: It makes no comment on copyright, so
probably an acknowledgement will suffice, particularly within PowerPoint
presentations. You do have to know the analogues for some things, e.g DsRed. To
copy image info use the keyboard PrintScreen button and paste into Photoshop to
edit out what you need. https://www.micro-shop.zeiss.com/us/us_en/spektral.php?cp_sid=&f=db Also check out the flow cytometry site for
their specific fluorescence surface type markers. http://www.bdbiosciences.com/colors/fluorescence_spectrum_viewer Regards Keith PS found a nice link when looking for the
Zeiss Spectra Viewer: http://www.analytchem.tugraz.at/fluorophores/links.php --------------------------------------------------------------------------- From:
Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Johan Henriksson Hello, Sorry misread your question, missed the open source bit. Keith --------------------------------------------------------------------------- Dr Keith J. Morris, Molecular Cytogenetics and Microscopy Core, Laboratory 00/069 and 00/070, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, United Kingdom. Telephone: +44 (0)1865 287568 Email: [hidden email] Web-pages: http://www.well.ox.ac.uk/cytogenetics/ -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Guillermo Palchik Sent: 11 May 2009 21:07 To: [hidden email] Subject: Re: fluorophore data? http://fluorescence.nexus-solutions.net/frames6.htm On May 11, 2009, at 1:40 PM, Johan Henriksson wrote: > Hello, > I've been looking around for emission & absorption spectras for all > common fluorophores but the data seems rather spread out. does > anyone know of a compilation that legally could be included in an > open source program? > > on a related note, can measured data be copyrighted? > > thanks > /Johan [hidden email] Sent from my iPhone good for you. I don't have one :( Guillermo Palchik wrote: > Sent from my iPhone > Is this what they call "twitter"? :) I especially appreciated the link declaring it as spam. >Sent from my iPhone -- So are we supposed to organise a whip-round? Guy Optical Imaging Techniques in Cell Biology by Guy Cox CRC Press / Taylor & Francis http://www.guycox.com/optical.htm ______________________________________________ Associate Professor Guy Cox, MA, DPhil(Oxon) Electron Microscope Unit, Madsen Building F09, University of Sydney, NSW 2006 ______________________________________________ Phone +61 2 9351 3176 Fax +61 2 9351 7682 Mobile 0413 281 861 ______________________________________________ http://www.guycox.net -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Nuno Moreno Sent: Tuesday, 12 May 2009 9:45 PM To: [hidden email] Subject: good for you. I don't have one :( Guillermo Palchik wrote: > Sent from my iPhone > Internal Virus Database is out-of-date. Checked by AVG. Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM Internal Virus Database is out-of-date. Checked by AVG. Version: 7.5.557 / Virus Database: 270.12.11/2089 - Release Date: 30/04/2009 5:53 PM Dear Confocalists! Sorry for the Iphone email -- I did not mean to send it! Guillermo On May 12, 2009, at 8:28 AM, Robert J. Palmer Jr. wrote: > Is this what they call "twitter"? :) > I especially appreciated the link declaring it as spam. > >> Sent from my iPhone > > > -- Guillermo Palchik [hidden email] Course Announcement Title: Optical Microscopy and Imaging in the Biomedical Sciences When: October 6 - October 15, 2009 Where: Marine Biology Laboratory, Woods Hole, MA, USA Tuition: $2750 (Includes room and board, text, handouts, supplies) Application Deadline: Applications accepted through June 26,2009 Admission application and information: Carol Hamel, Admissions Coordinator Marine Biological Laboratory 7 MBL Street Woods Hole, MA 02543-1015 (508) 289-7401 Internet: [hidden email] WWW: http://www.mbl.edu (Application forms available via Adobe Acrobat) Course Directors: Robert Hard, University at Buffalo Phone: [716] 829-3521 Email: [hidden email] Colin S. Izzard, State University of New York @ Albany Phone: [518] 442 - 4367 Email: [hidden email] Course Description: For Whom: Designed primarily for research scientists, physicians, postdoctoral trainees and advanced graduate students in animal, plant, medical and material sciences. Non-biologists seeking a comprehensive introduction to microscopy and digital imaging will benefit greatly from this course as well. There are no specific prerequisites, but an understanding of the basic principles of optics and imaging experience is desirable. Limited to 25 students. The nine day course consists of lectures, hands-on laboratory exercises, problem sets, laboratory demonstrations and discussions that will enable the participant to obtain and interpret microscope images of high quality, to perform quantitative optical measurements, and to produce digital records for documentation and analysis. For some structured exercises, students, as small groups, will acquire, analyze, interpret and present imaging data, thereby demonstrating their progress toward mastering microscopic/imaging skills. Live and fixed specimens will be provided for use in laboratory exercises and demonstrations. HOWEVER, STUDENTS ARE ENCOURAGED TO BRING THEIR OWN BIOLOGICAL (PRIMARY CULTURES, CELL LINES, PREPARED SLIDES, ETC.) AND MATERIAL SPECIMENS AND TO USE THEM THROUGHOUT THE COURSE FOR EXERCISES, WHERE APPROPRIATE. STUDENTS ALSO ARE ENCOURAGED TO MAKE APPOINTMENTS WITH OUR EXPERIENCED COMMERCIAL FACULTY TO EXAMINE THEIR CELLS OUTSIDE OF SCHEDULED CLASSES USING THE STATE OF THE ART EQUIPMENT AVAILABLE TO THEM. Cell culture facilities are available for student use. Students are highly encouraged to discuss their individual research problems with the academic and commercial faculty. Topics to be covered include: Principles of microscope design and image formation Bright field, dark field, phase contrast, polarized light, differential interference contrast, interference reflection, and fluorescence microscopy Digital imaging, recording, enhancement, processing and analysis Advanced fluorescent techniques (eg. FRAP, FLIP, FRET, FLIM, fluorescence polarization, multi-spectral and ratiometric imaging) Confocal scanning microscopy, multiphoton excitation fluorescence microscopy and structured illumination systems Image deconvolution, digital image restoration and 3-D reconstruction 3D image acquisition, display, and analysis Live cell imaging Laser microdissection and microcapture of tissues/cells for molecular biology. Super-resolution techniques (4PI, STED, PALM, etc.) Applications to live cells will be emphasized; other specimens will be covered as well. Students will have direct hands-on experience with state-of-the-art microscopes, digital cameras, photometers, and image acquisition/processing/analysis software provided by major optical, electronics, and software companies. Instruction will be provided by experienced staff from universities and industry. For faculty list and additional information, see: http://www.mbl.edu/education/courses/special_topics/om.html Hi all, I’m new to this program and was wondering if there is
any book on MetaMorph that I can look at as a reference. Thanks. Roland Roland Hilgarth Emory University Hi Roland,
I do not know of any books, but there are regular training
courses ran my Molecular Devices. See http://www.moleculardevices.com/pages/MM-new/meta_training.html for
dates etc. I have been told these are very good courses, i have not been to one
myself as I am in Australia and they don't run them here
(yet?).
Also the MetaMorph website has a good collection of support
articles in their knoledge base (see http://support.meta.moleculardevices.com/search.php)
There is also a big user base. If you get stuck just as a
question here (or you can contact me directly) and you will get the help you
need.
I will make the obligatory statement: I ahave no comericial
interest in Molecular Device, i am just a very satisified
customer.
Cheers
Cam
Cameron J.
Nowell From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Roland Hilgarth Sent: Wednesday, 13 May 2009 6:44 AM To: [hidden email] Subject: Anybody know of any book on MetaMorph imaging software Hi all, I’m new to this program and was wondering if there is any
book on MetaMorph that I can look at as a reference.
Thanks. Roland Roland Hilgarth Emory University This communication is intended only for the named recipient and may contain information that is confidential, legally privileged or subject to copyright; the Ludwig Institute for Cancer Research Ltd does not waiver any rights if you have received this communication in error. The views expressed in this communication are those of the sender and do not necessarily reflect the views of the Ludwig Institute for Cancer Research Ltd. |
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