Confocal Microscopy List

Re: CONFOCALMICROSCOPY Digest - 18 Aug 2017 to 19 Aug 2017 (#2017-186)

classic Classic list List threaded Threaded
♦
♦
 1 message Options
Daniel White-2 Daniel White-2
Reply | Threaded
Open this post in threaded view
♦
♦
|

Re: CONFOCALMICROSCOPY Digest - 18 Aug 2017 to 19 Aug 2017 (#2017-186)

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Hi Gabor,

*** commercial response ****

try:

FtsZ treadmilling, live cell 2x resolution as already mentioned

plus:

Highlight on synaptonemal complex 3D-SIM imaging on the DeltaVision™ OMX
<https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1474658927107/litdoc29181562_20161016064916.pdf>
https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1474658927107/litdoc29181562_20161016064916.pdf

TIRF-SIM is really taking off, watch out for publications on eg caveolae,
where you can finally count them all in a live cell imaging situation, also
single virus particles resolved during infection.

and a ton of stuff here, just from OMX 3D SIM in 2015
Prominent 2015 DeltaVision™ OMX publications
<https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1473366256985/litdoc29190589_20161016060529.pdf>

https://www.gelifesciences.com/gehcls_images/GELS/Related%20Content/Files/1473366256985/litdoc29190589_20161016060529.pdf

Best

Dan






> -
>
> Date:    Sat, 19 Aug 2017 14:46:52 +0000
> From:    Csúcs  Gábor <[hidden email]>
> Subject: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear All,
>
> I would have a slightly provocative question to the community: Could you
> help me to identify significant/major biological discoveries that were
> clearly dependent on the availability of various super-resolution light
> microscopy methods? The PNAS paper from the Zhuang lab (2016) about the
> actin-spectrin ring is a good example for me but I am looking for further
> ones. Of course, we also use super-resolution techniques in our facility
> but my observation is that these are used rather to provide "one nice image
> for a publication" or "another piece in the puzzle of evidences" but they
> are not "game winners", they were not necessarily the major piece of
> evidence to prove a biological hypothesis. So I am looking for biological
> questions that could be answered "only"/mostly by the existing
> super-resolution methods.
>         Thanks a lot for your help!
>
> Greetings                       Gabor
>
> ------------------------------
>
> Date:    Sat, 19 Aug 2017 18:04:31 +0200
> From:    Jakub Chojnacki <[hidden email]>
> Subject: Re: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Gabor,
>
> I realise that I am promoting my own work here, but here is a clear example
> of a discovery of the novel mechanism important for HIV entry competence.
> This work would not be possible without a super-resolution microscopy
> approach (in this case STED).
> http://science.sciencemag.org/content/338/6106/524
>
> Hope this helps.
>
> Regards,
> Jakub
>
> On 19 August 2017 at 16:46, Csúcs Gábor <[hidden email]>
> wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> >
> > Dear All,
> >
> > I would have a slightly provocative question to the community: Could you
> > help me to identify significant/major biological discoveries that were
> > clearly dependent on the availability of various super-resolution light
> > microscopy methods? The PNAS paper from the Zhuang lab (2016) about the
> > actin-spectrin ring is a good example for me but I am looking for further
> > ones. Of course, we also use super-resolution techniques in our facility
> > but my observation is that these are used rather to provide "one nice
> image
> > for a publication" or "another piece in the puzzle of evidences" but they
> > are not "game winners", they were not necessarily the major piece of
> > evidence to prove a biological hypothesis. So I am looking for biological
> > questions that could be answered "only"/mostly by the existing
> > super-resolution methods.
> >         Thanks a lot for your help!
> >
> > Greetings                       Gabor
> >
> >
>
> ------------------------------
>
> Date:    Sat, 19 Aug 2017 20:43:54 +0200
> From:    Tanneke den Blaauwen <[hidden email]>
> Subject: Re: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear Gabor,
>
> The discovery that the FtsZ-ring in bacteria is not a ring and that FtsZ
> is tread milling… (although the latter was also shown by 3D-SIM)
>
> Buss, J., Coltharp, C., Huang, T., Pohlmeyer, C., Wang, S.-C., Hatem, C.,
> & Xiao, J. (2013). In vivo organization of the FtsZ-ring by ZapA and ZapB
> revealed by quantitative super-resolution microscopy. Molecular
> Microbiology, 89(6), 1099–1120. http://doi.org/10.1111/mmi.12331
> Yang, X., Lyu, Z., Miguel, A., McQuillen, R., Huang, K. C., & Xiao, J.
> (2017). GTPase activity-coupled treadmilling of the bacterial tubulin FtsZ
> organizes septal cell wall synthesis. Science (New York, NY), 355(6326),
> 744–747. http://doi.org/10.1126/science.aak9995 <http://doi.org/10.1126/
> science.aak9995>
>
>
> Kind regards,
>
> Tanneke
>
> > On 19 Aug 2017, at 16:46, Csúcs Gábor <[hidden email]>
> wrote:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> >
> > Dear All,
> >
> > I would have a slightly provocative question to the community: Could you
> help me to identify significant/major biological discoveries that were
> clearly dependent on the availability of various super-resolution light
> microscopy methods? The PNAS paper from the Zhuang lab (2016) about the
> actin-spectrin ring is a good example for me but I am looking for further
> ones. Of course, we also use super-resolution techniques in our facility
> but my observation is that these are used rather to provide "one nice image
> for a publication" or "another piece in the puzzle of evidences" but they
> are not "game winners", they were not necessarily the major piece of
> evidence to prove a biological hypothesis. So I am looking for biological
> questions that could be answered "only"/mostly by the existing
> super-resolution methods.
> >       Thanks a lot for your help!
> >
> > Greetings                     Gabor
> >
>
> -------"Guardare, meravigliarsi e tornar a guardare."-------------
>
>
> Dr. Tanneke den Blaauwen
> Associate prof.
> Bacterial Cell Biology
> Swammerdam Institute for Life Sciences
> Location O|2 second floor (2e19)
> University of Amsterdam
> Boelelaan 1108
> 1081 HZ Amsterdam, the Netherlands
> PO box 66
> 1000 AB Amsterdam
> The Netherlands
>
>
> [hidden email]
> 0031631978136 (in the office)
> 0031644888461 (elsewhere)
>
> For sending materials:
>  FNWI/SILS/Bacterial Cell Biology
> T. Siersma/T.den Blaauwen
> Goederenontvangst VU (W&N G-063)
> De Boelelaan 1085
> 1081 HV Amsterdam
> the Netherlands
>
> ------------------------------
>
> Date:    Sat, 19 Aug 2017 22:49:57 +0200
> From:    Alberto Diaspro <[hidden email]>
> Subject: Re: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> well, another example is related to
> the mechanisms of inhibitory synaptic plasticity
>
> see: https://www.researchgate.net/publication/312204377_
> Nanoscale_molecular_reorganization_of_the_inhibitory_postsynaptic_
> density_is_a_determinant_of_GABAergic_synaptic_potentiation
> best
> alby
>
> > Il giorno 19 ago 2017, alle ore 16:46, Csúcs Gábor <
> [hidden email]> ha scritto:
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > Post images on http://www.imgur.com and include the link in your
> posting.
> > *****
> >
> >
> > Dear All,
> >
> > I would have a slightly provocative question to the community: Could you
> help me to identify significant/major biological discoveries that were
> clearly dependent on the availability of various super-resolution light
> microscopy methods? The PNAS paper from the Zhuang lab (2016) about the
> actin-spectrin ring is a good example for me but I am looking for further
> ones. Of course, we also use super-resolution techniques in our facility
> but my observation is that these are used rather to provide "one nice image
> for a publication" or "another piece in the puzzle of evidences" but they
> are not "game winners", they were not necessarily the major piece of
> evidence to prove a biological hypothesis. So I am looking for biological
> questions that could be answered "only"/mostly by the existing
> super-resolution methods.
> >       Thanks a lot for your help!
> >
> > Greetings                     Gabor
> >
>
> ------------------------------
>
> Date:    Sat, 19 Aug 2017 17:05:55 -0400
> From:    [hidden email]
> Subject: Re: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Many surface receptors have been found to be clustered on the plasma
> membrane, while some were known to be clustered from electron microscopy
> studies, with PALM this was extended to live cells.
> see e.g. Lillemeier et al. http://www.nature.com/ni/
> journal/v11/n1/abs/ni.1832.html?foxtrotcallback=true
>
> We have shown on fixed cells that the B cell receptor, CD19 and CD22 are
> found in nanometer sized clusters.
> https://www.researchgate.net/publication/236053888_The_
> Actin_and_Tetraspanin_Networks_Organize_Receptor_
> Nanoclusters_to_Regulate_B_Cell_Receptor-Mediated_Signaling
> https://www.researchgate.net/publication/286924448_
> Nanoscale_organization_and_dynamics_of_the_siglec_CD22_cooperate_with_the_
> cytoskeleton_in_restraining_BCR_signalling
>
> Well, I think there will need more to be done in this area and only time
> will tell if these then count as significant/major biological discoveries...
>
> best wishes
>
> Andreas
>
>
>
>
> -----Original Message-----
> From: Csúcs Gábor <[hidden email]>
> To: CONFOCALMICROSCOPY <[hidden email]>
> Sent: Sat, 19 Aug 2017 15:53
> Subject: Usefulness of super-resolution?
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
>
> Dear All,
>
> I would have a slightly provocative question to the community: Could you
> help me to identify significant/major biological discoveries that were
> clearly dependent on the availability of various super-resolution light
> microscopy methods? The PNAS paper from the Zhuang lab (2016) about the
> actin-spectrin ring is a good example for me but I am looking for further
> ones. Of course, we also use super-resolution techniques in our facility
> but my observation is that these are used rather to provide "one nice image
> for a publication" or "another piece in the puzzle of evidences" but they
> are not "game winners", they were not necessarily the major piece of
> evidence to prove a biological hypothesis. So I am looking for biological
> questions that could be answered "only"/mostly by the existing
> super-resolution methods.
>         Thanks a lot for your help!
>
> Greetings                       Gabor
>
> ------------------------------
>
> End of CONFOCALMICROSCOPY Digest - 18 Aug 2017 to 19 Aug 2017 (#2017-186)
> *************************************************************************
>
Free forum by Nabble Edit this page