Re: CONFOCALMICROSCOPY Digest - 19 Jun 2012 to 20 Jun 2012 (#2012-145)

[Sebastian Peck]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Paul,

Why don't you try pumping your carbogen through some gas permeable
tubing placed inside your media?  I've seen it used for hydrating the
gas when going into imaging chambers from in-vivo scientific and I
believe that it will work the other way around.

Cheers,
Sebastian


On Wed, Jun 20, 2012 at 10:04 PM, CONFOCALMICROSCOPY automatic digest
system <[hidden email]> wrote:

> There is 1 message totalling 60 lines in this issue.
>
> Topics of the day:
>
>  1. oxygenate medium
>
> ----------------------------------------------------------------------
>
> Date:    Wed, 20 Jun 2012 14:56:16 +0200
> From:    ricardo figueroa <[hidden email]>
> Subject: Re: oxygenate medium
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Not talking from experience, but essentially bubbling is just to increase
> the surface in contact with the atmosphere for gas exchange and equilibrium
> to be reached. This should be possible to do by mearly having the media in
> the desired atmosphere with as much surface as possible exposed. This will
> probably not be as effective as bubbling but should work non the less. To
> speed up things I would design a (small box or scale up as necessary)
> container that is constantly supplied with the atmosphere and along the
> bottom a shallow but wide laminar flow of the medium (slightly tilting the
> box may be god for the flow). For monitoring the O2 levels an oxygen optode
> should do the trick.
>
> /Ricardo Figueroa
>
> On Tue, Jun 19, 2012 at 1:08 AM, Paul Herzmark <[hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi all,
>>
>> We do 2P microscopy of thick tissue explants. To deliver appropriate oxygen
>> and carbon dioxide levels to the deep parts of the tissue we bubble the
>> medium with 95% O2 and 5% CO2. That keeps the cells as happy as when they
>> are vascularized.
>>
>> Now we want to do the experiments with serum added to the medium, but the
>> extra protein causes the bubbling medium to foam too much.
>>
>> Any suggestions on how to increase the dissolved O2 and CO2 without the
>> foaming?
>> Also, any ideas on how to monitor the resulting O2 levels?
>>
>> Thanks so much.
>>
>>
>> Paul Herzmark
>>
>> [hidden email]
>> Department of Molecular and Cell Biology
>> 479 Life Science Addition
>> University of California, Berkeley
>> Berkeley, CA  94720-3200
>> [phone]
>>
>
> ------------------------------
>
> End of CONFOCALMICROSCOPY Digest - 19 Jun 2012 to 20 Jun 2012 (#2012-145)
> *************************************************************************

[Paul Herzmark]

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Thanks for that suggestion. I was thinking that might work. I even have
that In-Vivo scientific system.
Paul


On Thu, Jun 21, 2012 at 10:09 AM, Sebastian Peck <[hidden email]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Paul,
>
> Why don't you try pumping your carbogen through some gas permeable
> tubing placed inside your media?  I've seen it used for hydrating the
> gas when going into imaging chambers from in-vivo scientific and I
> believe that it will work the other way around.
>
> Cheers,
> Sebastian
>
>
> On Wed, Jun 20, 2012 at 10:04 PM, CONFOCALMICROSCOPY automatic digest
> system <[hidden email]> wrote:
> > There is 1 message totalling 60 lines in this issue.
> >
> > Topics of the day:
> >
> >  1. oxygenate medium
> >
> > ----------------------------------------------------------------------
> >
> > Date:    Wed, 20 Jun 2012 14:56:16 +0200
> > From:    ricardo figueroa <[hidden email]>
> > Subject: Re: oxygenate medium
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Not talking from experience, but essentially bubbling is just to increase
> > the surface in contact with the atmosphere for gas exchange and
> equilibrium
> > to be reached. This should be possible to do by mearly having the media
> in
> > the desired atmosphere with as much surface as possible exposed. This
> will
> > probably not be as effective as bubbling but should work non the less. To
> > speed up things I would design a (small box or scale up as necessary)
> > container that is constantly supplied with the atmosphere and along the
> > bottom a shallow but wide laminar flow of the medium (slightly tilting
> the
> > box may be god for the flow). For monitoring the O2 levels an oxygen
> optode
> > should do the trick.
> >
> > /Ricardo Figueroa
> >
> > On Tue, Jun 19, 2012 at 1:08 AM, Paul Herzmark <[hidden email]>
> wrote:
> >
> >> *****
> >> To join, leave or search the confocal microscopy listserv, go to:
> >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> >> *****
> >>
> >> Hi all,
> >>
> >> We do 2P microscopy of thick tissue explants. To deliver appropriate
> oxygen
> >> and carbon dioxide levels to the deep parts of the tissue we bubble the
> >> medium with 95% O2 and 5% CO2. That keeps the cells as happy as when
> they
> >> are vascularized.
> >>
> >> Now we want to do the experiments with serum added to the medium, but
> the
> >> extra protein causes the bubbling medium to foam too much.
> >>
> >> Any suggestions on how to increase the dissolved O2 and CO2 without the
> >> foaming?
> >> Also, any ideas on how to monitor the resulting O2 levels?
> >>
> >> Thanks so much.
> >>
> >>
> >> Paul Herzmark
> >>
> >> [hidden email]
> >> Department of Molecular and Cell Biology
> >> 479 Life Science Addition
> >> University of California, Berkeley
> >> Berkeley, CA  94720-3200
> >> [phone]
> >>
> >
> > ------------------------------
> >
> > End of CONFOCALMICROSCOPY Digest - 19 Jun 2012 to 20 Jun 2012 (#2012-145)
> > *************************************************************************
>