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Re: CONFOCALMICROSCOPY Digest - 2 Apr 2016 to 4 Apr 2016 (#2016-80)

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Re: CONFOCALMICROSCOPY Digest - 2 Apr 2016 to 4 Apr 2016 (#2016-80)

Mike is absolutely correct…habitual, overly generous application of oil can lead to penetration of the oil down into the optics.  Over the past 18 years, I have had to have 2 lenses rebuilt and 1 replaced.  Check with your manufacturer.  Some will rebuild lenses. Its not an inexpensive proposition, but it does usually cost less than replacing the lens.
Good luck.

Lee Cohen-Gould

Imaging Core Facility


Weill Cornell Medicine

Weill Cornell Medical College

From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> on behalf of CONFOCALMICROSCOPY automatic digest system <[hidden email]<mailto:[hidden email]>>
Reply-To: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>>
Date: Tuesday, April 5, 2016 at 1:03 AM
To: "[hidden email]<mailto:[hidden email]>" <[hidden email]<mailto:[hidden email]>>
Subject: CONFOCALMICROSCOPY Digest - 2 Apr 2016 to 4 Apr 2016 (#2016-80)

There are 27 messages totalling 2581 lines in this issue.

Topics of the day:

  1. Oil on back focal plane of objective (17)
  2. Postdoc position - OPTICAL CONTROL OF PROTEIN FUNCTION
  3. Water dispenser (4)
  4. double staining test.
  5. laser alignment issue FluoView1000
  6. Immersion oil (3)

----------------------------------------------------------------------

Date:    Mon, 4 Apr 2016 05:48:27 -0400
From:    Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Subject: Oil on back focal plane of objective

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*****

Hi All,

Trying not to be cynical here, but, can anybody please explain to me if
there is a way for a drop of oil to end up on the center of the back focal
plane of an objective (oil immersion objective) on an inverted scope.  No
smear from the side.

Thanks.

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

------------------------------

Date:    Mon, 4 Apr 2016 10:51:12 +0000
From:    "MODEL, MICHAEL" <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

Oil can sometimes leak inside objectives, especially on those with anti-sho=
ck... - not sure what it's called, when the central part can move up and do=
wn. We once had to throw away one quite expensive piece. It helps to wipe o=
bjectives after use.
Mike

________________________________________
From: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> on behalf=
of Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Sent: Monday, April 4, 2016 5:48 AM
To: [hidden email]<mailto:[hidden email]>
Subject: Oil on back focal plane of objective

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*****

Hi All,

Trying not to be cynical here, but, can anybody please explain to me if
there is a way for a drop of oil to end up on the center of the back focal
plane of an objective (oil immersion objective) on an inverted scope.  No
smear from the side.

Thanks.

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

------------------------------

Date:    Mon, 4 Apr 2016 08:26:59 -0400
From:    Philip Oshel <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

I've noticed the same thing.
Discarding the grumpy usual, on the basis of not having "people like
that" using the confocals, the only thing I can think of is minute
pinholes in the mounting cement allowing the oil to soak through - by
gravity if on on inverted microscope, or capillary action if on an upright.

Phil

On 04/04/2016 05:48 , Gary Laevsky wrote:
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*****

Hi All,

Trying not to be cynical here, but, can anybody please explain to me if
there is a way for a drop of oil to end up on the center of the back focal
plane of an objective (oil immersion objective) on an inverted scope.  No
smear from the side.

Thanks.


--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576

------------------------------

Date:    Mon, 4 Apr 2016 06:09:18 -0400
From:    John Oreopoulos <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

I've never experienced it myself, but if it's a spring-loaded objective =
barrel, oil can potentially leak through the gap between the =
spring-loaded section and the outside barrel and make it's way down =
through the objective - maybe. This is why I wipe oil off objectives as =
soon as I'm done using them. Hard to imagine any other way oil could get =
there, unless maybe oil dropped onto the nosepiece at the base of the =
objective? Or maybe someone removed the objective to clean it and =
accidentally set it down on a surface with oil? In any case, it should =
be fine to clean it off it it's only on the outside surface of the =
bottom lens of the objective.

Sending good vibes your way,

John Oreopoulos




On 2016-04-04, at 5:48 AM, Gary Laevsky wrote:

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posting.
*****
=20
Hi All,
=20
Trying not to be cynical here, but, can anybody please explain to me =
if
there is a way for a drop of oil to end up on the center of the back =
focal
plane of an objective (oil immersion objective) on an inverted scope.  =
No
smear from the side.
=20
Thanks.
=20
--=20
Best,
=20
Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

------------------------------

Date:    Mon, 4 Apr 2016 12:57:17 +0000
From:    Guy Cox <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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------------------------------

Date:    Mon, 4 Apr 2016 10:39:20 -0400
From:    Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

Hi All,

To be clear, if oil was leaking down the barrel of the objective, and ended
up on the center of the back focal plan, there would be a trail from the
edge, right?

This was a solitary drop on the center of the back.

I'm trying to find someone that will correct me from thinking this was a
malicious act.

Best,

gary

On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
wrote:

Hi All,

Trying not to be cynical here, but, can anybody please explain to me if
there is a way for a drop of oil to end up on the center of the back focal
plane of an objective (oil immersion objective) on an inverted scope.  No
smear from the side.

Thanks.

--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310




--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

------------------------------

Date:    Mon, 4 Apr 2016 09:46:45 -0500
From:    Jordan T Becker <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

Hey,

What about inexperienced or rushed hands? Oil on a finger accidentally
grazing the bottom of the rear lens when placing/installing or removing the
objective? Unless it's clearly a drop...is it a smudge/print shape?

On Mon, Apr 4, 2016 at 9:39 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
wrote:

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*****

Hi All,

To be clear, if oil was leaking down the barrel of the objective, and ended
up on the center of the back focal plan, there would be a trail from the
edge, right?

This was a solitary drop on the center of the back.

I'm trying to find someone that will correct me from thinking this was a
malicious act.

Best,

gary

On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
wrote:

> Hi All,
>
> Trying not to be cynical here, but, can anybody please explain to me if
> there is a way for a drop of oil to end up on the center of the back
focal

> plane of an objective (oil immersion objective) on an inverted scope.  No
> smear from the side.
>
> Thanks.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>



--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310


------------------------------

Date:    Mon, 4 Apr 2016 17:15:12 +0200
From:    Zdenek Svindrych <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
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*****

=0A=
Hi.=0A=
To me it seems very unlikely someone would do that on purpose. It's more =
=0A=
likely that some greenhorn user misunderstood the sentence "don't forget t=
o =0A=
apply oil to the oil immersion objective" or something like that in your =
=0A=
manual (I'm sure you don't specifically note that it's not necessary to =
=0A=
unscrew the objective from the turret to do that :-).=0A=
But seriously, if the oil got inside the objective through the front lens =
=0A=
seal and found its way to the last lens, the lens is probably full of oil =
=0A=
and can't be fixed. You may try to clean it and test whether it performs =
=0A=
well, or ask the vendor whether they can check it for you (and ask how muc=
h =0A=
it would cost!). If you have service contract, they might do it for you fo=
r =0A=
free, you might even be covered by some sort of warranty.=0A=
=0A=
I doubt oil could get there from the lens threads through capillary action=
, =0A=
and oil is not superfluid...=0A=
=0A=
Good Luck!=0A=
=0A=
zdenek=0A=
=0A=
=0A=
---------- P=C5=AFvodn=C3=AD zpr=C3=A1va ----------=0A=
Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>=0A=
Komu: [hidden email]=0A=<mailto:[hidden email]=0A=>
Datum: 4. 4. 2016 10:39:50=0A=
P=C5=99edm=C4=9Bt: Re: Oil on back focal plane of objective=0A=
=0A=
"*****=0A=
To join, leave or search the confocal microscopy listserv, go to:=0A=
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy-3D0A-3D&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=WRm88MaBRfRPMlvgBBIJqOjT7jAGSxqUAXpPJJsNboc&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.=
=0A=
*****=0A=
=0A=
Hi All,=0A=
=0A=
To be clear, if oil was leaking down the barrel of the objective, and ende=
d=0A=
up on the center of the back focal plan, there would be a trail from the=
=0A=
edge, right?=0A=
=0A=
This was a solitary drop on the center of the back.=0A=
=0A=
I'm trying to find someone that will correct me from thinking this was a=
=0A=
malicious act.=0A=
=0A=
Best,=0A=
=0A=
gary=0A=
=0A=
On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>=
=0A=
wrote:=0A=
=0A=
Hi All,=0A=
=0A=
Trying not to be cynical here, but, can anybody please explain to me if=
=0A=
there is a way for a drop of oil to end up on the center of the back foc=
al=0A=
plane of an objective (oil immersion objective) on an inverted scope. No=
=0A=
smear from the side.=0A=
=0A=
Thanks.=0A=
=0A=
--=0A=
Best,=0A=
=0A=
Gary Laevsky, Ph.D.=0A=
Director, Confocal Imaging Facility=0A=
Nikon Center of Excellence=0A=
Dept. of Molecular Biology=0A=
Washington Rd.=0A=
Princeton University=0A=
Princeton, New Jersey, 08544-1014=0A=
(O) 609 258 5432=0A=
(C) 508 507 1310=0A=
=0A=
=0A=
=0A=
=0A=
-- =0A=
Best,=0A=
=0A=
Gary Laevsky, Ph.D.=0A=
Director, Confocal Imaging Facility=0A=
Nikon Center of Excellence=0A=
Dept. of Molecular Biology=0A=
Washington Rd.=0A=
Princeton University=0A=
Princeton, New Jersey, 08544-1014=0A=
(O) 609 258 5432=0A=
(C) 508 507 1310"=0A=

------------------------------

Date:    Mon, 4 Apr 2016 11:43:53 -0400
From:    Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

Ok, I'm going with oil leaking down the side of the barrel, and the trail
disappeared (I would have xpected to see the trail).

Thanks for the clarity, and restoration.

Best,

Gary

On Mon, Apr 4, 2016 at 11:15 AM, Zdenek Svindrych <[hidden email]<mailto:[hidden email]>> wrote=
:

*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****


Hi.
To me it seems very unlikely someone would do that on purpose. It's more
likely that some greenhorn user misunderstood the sentence "don't forget =
to
apply oil to the oil immersion objective" or something like that in your
manual (I'm sure you don't specifically note that it's not necessary to
unscrew the objective from the turret to do that :-).
But seriously, if the oil got inside the objective through the front lens
seal and found its way to the last lens, the lens is probably full of oil
and can't be fixed. You may try to clean it and test whether it performs
well, or ask the vendor whether they can check it for you (and ask how mu=
ch
it would cost!). If you have service contract, they might do it for you f=
or
free, you might even be covered by some sort of warranty.

I doubt oil could get there from the lens threads through capillary actio=
n,
and oil is not superfluid...

Good Luck!

zdenek


---------- P=C5=AFvodn=C3=AD zpr=C3=A1va ----------
Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Komu: [hidden email]<mailto:[hidden email]>
Datum: 4. 4. 2016 10:39:50
P=C5=99edm=C4=9Bt: Re: Oil on back focal plane of objective

"*****
To join, leave or search the confocal microscopy listserv, go to:
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

Hi All,

To be clear, if oil was leaking down the barrel of the objective, and end=
ed
up on the center of the back focal plan, there would be a trail from the
edge, right?

This was a solitary drop on the center of the back.

I'm trying to find someone that will correct me from thinking this was a
malicious act.

Best,

gary

On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
wrote:

> Hi All,
>
> Trying not to be cynical here, but, can anybody please explain to me if
> there is a way for a drop of oil to end up on the center of the back
focal
> plane of an objective (oil immersion objective) on an inverted scope. N=
o

> smear from the side.
>
> Thanks.
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310
>



--
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310"




--=20
Best,

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310

------------------------------

Date:    Mon, 4 Apr 2016 15:31:41 +0000
From:    David Claypool <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
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Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

To All,

This is a somewhat common problem with oil immersion objectives on inverted=
microscopes and is not limited to any one brand or manufacturer.=20

If excess oil is not removed after using the objective m(a simple wipe with=
lens tissue will suffice), gravity will eventually cause the oil to run do=
wn the spring loaded front lens barrel into the objective. Over time enough=
oil will build up in the objective, so that it can find its way between th=
e inner lens elements.

The other scenario is if the front lens of the objective has been crashed i=
nto a specimen holder or the underside of a x-y stage. This can cause the s=
eal around the front lens to develop a microcrack and the immersion oil wil=
l find its way into the objective. =20

Either of the above damage is usually repairable by the manufacturer, but n=
ot under warranty.

I have seen people use a piece of a pipe cleaner wrapped around the objecti=
ve to catch the excess oil, but you need to remember to change it regularly=
.

David J. Claypool
Digital Imaging Product Manager
Micro Video Instruments
Office: 800-875-2041 x5221
Cell: 603-809-5342
[hidden email]<mailto:[hidden email]>

On Apr 4, 2016, at 11:16 AM, Zdenek Svindrych <[hidden email]<mailto:[hidden email]>> wrote:
=20
*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
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*****
=20
=20
Hi.
To me it seems very unlikely someone would do that on purpose. It's more=
=20
likely that some greenhorn user misunderstood the sentence "don't forget =
to=20
apply oil to the oil immersion objective" or something like that in your=
=20
manual (I'm sure you don't specifically note that it's not necessary to=20
unscrew the objective from the turret to do that :-).
But seriously, if the oil got inside the objective through the front lens=
=20
seal and found its way to the last lens, the lens is probably full of oil=
=20
and can't be fixed. You may try to clean it and test whether it performs=
=20
well, or ask the vendor whether they can check it for you (and ask how mu=
ch=20
it would cost!). If you have service contract, they might do it for you f=
or=20
free, you might even be covered by some sort of warranty.
=20
I doubt oil could get there from the lens threads through capillary actio=
n,=20
and oil is not superfluid...
=20
Good Luck!
=20
zdenek
=20
=20
---------- P=F9vodn=ED zpr=E1va ----------
Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Komu: [hidden email]<mailto:[hidden email]>
Datum: 4. 4. 2016 10:39:50
P=F8edm=ECt: Re: Oil on back focal plane of objective
=20
"*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****
=20
Hi All,
=20
To be clear, if oil was leaking down the barrel of the objective, and end=
ed
up on the center of the back focal plan, there would be a trail from the
edge, right?
=20
This was a solitary drop on the center of the back.
=20
I'm trying to find someone that will correct me from thinking this was a
malicious act.
=20
Best,
=20
gary
=20
On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
wrote:
=20
Hi All,
=20
Trying not to be cynical here, but, can anybody please explain to me if
there is a way for a drop of oil to end up on the center of the back foc=
al
plane of an objective (oil immersion objective) on an inverted scope. No
smear from the side.
=20
Thanks.
=20
--
Best,
=20
Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310
=20
=20
=20
--=20
Best,
=20
Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310"

------------------------------

Date:    Mon, 4 Apr 2016 10:54:00 -0600
From:    Craig Brideau <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
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*****

I still stand by my recommendation of cloth hair scrunchies on the
objectives. It catches any oil drips running down the side of the objective
on inverted scopes. It won't save you from oil leaking into the
spring-loaded tips but it at least keeps it from soaking into the optics
under the objective. You can even use them for color coding!
https://urldefense.proofpoint.com/v2/url?u=http-3A__previews.123rf.com_images_belchonock_belchonock1108_belchonock110800-3D&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=2B7vC3e9fqybB26zIpurO-_MOQolUbDtuRsQIUxZAcQ&e=
172/10220322-hair-scrunchies-on-brown-background-Stock-Photo.jpg

Craig Brideau

On Mon, Apr 4, 2016 at 9:31 AM, David Claypool <[hidden email]<mailto:[hidden email]>> wrote:

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*****

To All,

This is a somewhat common problem with oil immersion objectives on
inverted microscopes and is not limited to any one brand or manufacturer.

If excess oil is not removed after using the objective m(a simple wipe
with lens tissue will suffice), gravity will eventually cause the oil to
run down the spring loaded front lens barrel into the objective. Over tim=
e
enough oil will build up in the objective, so that it can find its way
between the inner lens elements.

The other scenario is if the front lens of the objective has been crashed
into a specimen holder or the underside of a x-y stage. This can cause th=
e
seal around the front lens to develop a microcrack and the immersion oil
will find its way into the objective.

Either of the above damage is usually repairable by the manufacturer, but
not under warranty.

I have seen people use a piece of a pipe cleaner wrapped around the
objective to catch the excess oil, but you need to remember to change it
regularly.

David J. Claypool
Digital Imaging Product Manager
Micro Video Instruments
Office: 800-875-2041 x5221
Cell: 603-809-5342
[hidden email]<mailto:[hidden email]>

> On Apr 4, 2016, at 11:16 AM, Zdenek Svindrych <[hidden email]<mailto:[hidden email]>> wrote=
:
>
> *****
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posting.
> *****
>
>
> Hi.
> To me it seems very unlikely someone would do that on purpose. It's mor=
e
> likely that some greenhorn user misunderstood the sentence "don't forge=
t
to
> apply oil to the oil immersion objective" or something like that in you=
r
> manual (I'm sure you don't specifically note that it's not necessary to
> unscrew the objective from the turret to do that :-).
> But seriously, if the oil got inside the objective through the front le=
ns
> seal and found its way to the last lens, the lens is probably full of o=
il
> and can't be fixed. You may try to clean it and test whether it perform=
s
> well, or ask the vendor whether they can check it for you (and ask how
much
> it would cost!). If you have service contract, they might do it for you
for
> free, you might even be covered by some sort of warranty.
>
> I doubt oil could get there from the lens threads through capillary
action,

> and oil is not superfluid...
>
> Good Luck!
>
> zdenek
>
>
> ---------- P=C5=AFvodn=C3=AD zpr=C3=A1va ----------
> Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>
> Komu: [hidden email]<mailto:[hidden email]>
> Datum: 4. 4. 2016 10:39:50
> P=C5=99edm=C4=9Bt: Re: Oil on back focal plane of objective
>
> "*****
> To join, leave or search the confocal microscopy listserv, go to:
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posting.
> *****
>
> Hi All,
>
> To be clear, if oil was leaking down the barrel of the objective, and
ended
> up on the center of the back focal plan, there would be a trail from th=
e
> edge, right?
>
> This was a solitary drop on the center of the back.
>
> I'm trying to find someone that will correct me from thinking this was =
a

> malicious act.
>
> Best,
>
> gary
>
> On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky <[hidden email]<mailto:[hidden email]>>
> wrote:
>
>> Hi All,
>>
>> Trying not to be cynical here, but, can anybody please explain to me i=
f
>> there is a way for a drop of oil to end up on the center of the back
focal
>> plane of an objective (oil immersion objective) on an inverted scope. =
No

>> smear from the side.
>>
>> Thanks.
>>
>> --
>> Best,
>>
>> Gary Laevsky, Ph.D.
>> Director, Confocal Imaging Facility
>> Nikon Center of Excellence
>> Dept. of Molecular Biology
>> Washington Rd.
>> Princeton University
>> Princeton, New Jersey, 08544-1014
>> (O) 609 258 5432
>> (C) 508 507 1310
>
>
>
> --
> Best,
>
> Gary Laevsky, Ph.D.
> Director, Confocal Imaging Facility
> Nikon Center of Excellence
> Dept. of Molecular Biology
> Washington Rd.
> Princeton University
> Princeton, New Jersey, 08544-1014
> (O) 609 258 5432
> (C) 508 507 1310"


------------------------------

Date:    Mon, 4 Apr 2016 17:11:36 +0000
From:    Christian Elowsky <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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------------------------------

Date:    Mon, 4 Apr 2016 11:16:04 -0600
From:    Craig Brideau <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

Glad you liked it! Just remember to change them periodically, they can get
quite messy over time!

Craig

On Mon, Apr 4, 2016 at 11:11 AM, Christian Elowsky <[hidden email]<mailto:[hidden email]>>
wrote:

Craig,

You just made my day, if not month with this suggestion!

Thank you!

Christian



-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Craig Brideau
Sent: Monday, April 04, 2016 11:54 AM
To: [hidden email]<mailto:[hidden email]>
Subject: Re: Oil on back focal plane of objective

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

I still stand by my recommendation of cloth hair scrunchies on the
objectives. It catches any oil drips running down the side of the objecti=
ve
on inverted scopes. It won't save you from oil leaking into the
spring-loaded tips but it at least keeps it from soaking into the optics
under the objective. You can even use them for color coding!

https://urldefense.proofpoint.com/v2/url?u=http-3A__previews.123rf.com_images_belchonock_belchonock1108_belchonock1108-3D&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=1MgpHMIFl7Gb4CqJ8UkIDWJjrgZLg2O0ZJw5nvJ8lLc&e=
00172/10220322-hair-scrunchies-on-brown-background-Stock-Photo.jpg

Craig Brideau

On Mon, Apr 4, 2016 at 9:31 AM, David Claypool <[hidden email]<mailto:[hidden email]>> wrote=
:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
> Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your
posting.
> *****
>
> To All,
>
> This is a somewhat common problem with oil immersion objectives on
> inverted microscopes and is not limited to any one brand or manufacture=
r.

>
> If excess oil is not removed after using the objective m(a simple wipe
> with lens tissue will suffice), gravity will eventually cause the oil
> to run down the spring loaded front lens barrel into the objective.
> Over time enough oil will build up in the objective, so that it can
> find its way between the inner lens elements.
>
> The other scenario is if the front lens of the objective has been
> crashed into a specimen holder or the underside of a x-y stage. This
> can cause the seal around the front lens to develop a microcrack and
> the immersion oil will find its way into the objective.
>
> Either of the above damage is usually repairable by the manufacturer,
> but not under warranty.
>
> I have seen people use a piece of a pipe cleaner wrapped around the
> objective to catch the excess oil, but you need to remember to change
> it regularly.
>
> David J. Claypool
> Digital Imaging Product Manager
> Micro Video Instruments
> Office: 800-875-2041 x5221
> Cell: 603-809-5342
> [hidden email]<mailto:[hidden email]>
>
> > On Apr 4, 2016, at 11:16 AM, Zdenek Svindrych <[hidden email]<mailto:[hidden email]>>
wrote:

> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
> > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your
> posting.
> > *****
> >
> >
> > Hi.
> > To me it seems very unlikely someone would do that on purpose. It's
> > more likely that some greenhorn user misunderstood the sentence
> > "don't forget
> to
> > apply oil to the oil immersion objective" or something like that in
> > your manual (I'm sure you don't specifically note that it's not
> > necessary to unscrew the objective from the turret to do that :-).
> > But seriously, if the oil got inside the objective through the front
> > lens seal and found its way to the last lens, the lens is probably
> > full of oil and can't be fixed. You may try to clean it and test
> > whether it performs well, or ask the vendor whether they can check
> > it for you (and ask how
> much
> > it would cost!). If you have service contract, they might do it for
> > you
> for
> > free, you might even be covered by some sort of warranty.
> >
> > I doubt oil could get there from the lens threads through capillary
> action,
> > and oil is not superfluid...
> >
> > Good Luck!
> >
> > zdenek
> >
> >
> > ---------- P=C5=AFvodn=C3=AD zpr=C3=A1va ----------
> > Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>
> > Komu: [hidden email]<mailto:[hidden email]>
> > Datum: 4. 4. 2016 10:39:50
> > P=C5=99edm=C4=9Bt: Re: Oil on back focal plane of objective
> >
> > "*****
> > To join, leave or search the confocal microscopy listserv, go to:
> > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=QSSHt-X-tGTDIRu7LPf4UB8aMoO7kzZYbCd_yZxjruQ&e=
> > Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your
> posting.
> > *****
> >
> > Hi All,
> >
> > To be clear, if oil was leaking down the barrel of the objective,
> > and
> ended
> > up on the center of the back focal plan, there would be a trail from
> > the edge, right?
> >
> > This was a solitary drop on the center of the back.
> >
> > I'm trying to find someone that will correct me from thinking this
> > was a malicious act.
> >
> > Best,
> >
> > gary
> >
> > On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky
> > <[hidden email]<mailto:[hidden email]>>
> > wrote:
> >
> >> Hi All,
> >>
> >> Trying not to be cynical here, but, can anybody please explain to
> >> me if there is a way for a drop of oil to end up on the center of
> >> the back
> focal
> >> plane of an objective (oil immersion objective) on an inverted
> >> scope. No smear from the side.
> >>
> >> Thanks.
> >>
> >> --
> >> Best,
> >>
> >> Gary Laevsky, Ph.D.
> >> Director, Confocal Imaging Facility Nikon Center of Excellence
> >> Dept. of Molecular Biology Washington Rd.
> >> Princeton University
> >> Princeton, New Jersey, 08544-1014
> >> (O) 609 258 5432
> >> (C) 508 507 1310
> >
> >
> >
> > --
> > Best,
> >
> > Gary Laevsky, Ph.D.
> > Director, Confocal Imaging Facility
> > Nikon Center of Excellence
> > Dept. of Molecular Biology
> > Washington Rd.
> > Princeton University
> > Princeton, New Jersey, 08544-1014
> > (O) 609 258 5432
> > (C) 508 507 1310"
>


------------------------------

Date:    Mon, 4 Apr 2016 10:43:13 -0700
From:    "Quintanar, Loren" <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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------------------------------

Date:    Mon, 4 Apr 2016 13:30:34 -0500
From:    Clive Bramham <[hidden email]<mailto:[hidden email]>>
Subject: Postdoc position - OPTICAL CONTROL OF PROTEIN FUNCTION

*****
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*****

At the Department of Biomedicine, University of Bergen, Norway, a full-ti=
me temporary=20
postdoctoral position in Cellular Neuroscience is available for three (3)=
years.
=20
The position is part of the Top Research project =93Molecular control of =
Arc protein:=20
decoding a master regulator of synaptic plasticity and cognition=94, fund=
ed by the Research=20
Council of Norway. The project is led by Professor Clive Bramham of the N=
euroscience=20
Research Group.
=20
Project
The project team is multidisciplinary with expertise in molecular and cel=
lular=20
neuroscience, protein structural biology, protein biophysics, and human g=
enetics.
=20
This postdoctoral project focuses on optical methods for regulating prote=
in activity.
=20
For further information about the project and the position, please contac=
t Professor Clive=20
R. Bramham, phone: +47 55 58 60 32 or e-mail: [hidden email]<mailto:[hidden email]>
=20
Qualifications and qualities
The applicant must have a doctoral degree in cellular or molecular neuros=
cience, live-cell=20
imaging technology, or a related discipline.
The applicant must have a track-record of high-quality scientific publish=
ing.
The successful applicant must have experience in the use and development =
of fluorescent=20
fusion proteins for live-cell imaging applications.
Experience with FRET imaging techniques and superresolution microscopy is=
an=20
advantage.
Experience with projects in synaptic cell biology is an advantage.
The candidate should be proficient in written and oral English
Personal skills will be emphasized. The candidate should be able to work =
independently=20
and interactively in a team setting, be motivated and responsible, and al=
so have a great=20
work capacity and enthusiasm for research

Benefits include:
Salary level 57 (code 1352/pay framework 24.1) at present NOK 483 700 gro=
ss p.a., with=20
a degree in Medicine or Dentistry level 59 (code 1352/pay framework 24.3)=
at present=20
NOK 501 300 gross p.a., with a medical specialization level 61 (code 1352=
/pay framework=20
24.5) at present NOK 519 800 gross p.a. on the government salary scale. F=
urther=20
promotion will be after service seniority in the position.
A good pension scheme in the Norwegian Public Service Pension Fund.
A position in an inclusive workplace (IA enterprise)
Good welfare benefits

Applications must be made through the jobbnorge website. (www.jobbnorge.n=
o/), position=20
ID 123781.
You send your application with attachments electronically via =93JOBBNORG=
E=94 by clicking on=20
the button marked =93APPLY FOR THIS JOB=94.

The application must contain:

a brief letter of application stating your motivation for the position an=
d why you are=20
applying an overview of your education and work experience (CV)
witnessed copies of diplomas and relevant certificates (applicants with e=
ducation from=20
other countries than Norway must enclose witnessed diplomas in both the o=
riginal=20
language and authorized translations) complete list of publications publi=
cations two=20
referees (name and contact information) Please notice that the applicatio=
ns will be=20
assessed only with the information available in =93JobbNorge=94 when the =
deadline expires. It=20
is the applicant`s responsibility to ensure that all relevant attachments=
are submitted by=20
the deadline.

Please notice that applications sent by e-mail will not be considered.

Closing date for applications: May 1, 2016.

------------------------------

Date:    Mon, 4 Apr 2016 20:58:13 +0200
From:    Ralf Palmisano <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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*****

Hi,

I am not cynical and I don't mean to be mean to anyone here. I will just
name facts, eventually ask a "rhetorical" question and leave the rest up
to you.

- A nice solitary drop on the backfocal plane of an inverted microscope
can physically only happen, if the turret is not being used for quite a
while.
Was this the case?
- If no smear is on the outside, it must have been making its way
through the objectives inner parts and lenses.
- If the oil went down the outside, no matter what better how long you
wait, there will always be a smear trail. Only just not if you have not
used it for about a year or two.
Did you?
- How does one realise there is a drop of oil on the backfocal plane?
I would guess because a user or member of staff realises you only get a
a distorted image, whatever you do?
- If such an amount of oil leaks through the inner parts of an objective
lens you will already realise a visible impairment of your light passing
through the objective lens long before oil getting to the back focal
plane, also quite early leading to a distorted image.
Unless all your users are blind or have no clue what they are imaging or
looking at.
You can easily check the front lens of an objective using a stereo or
simple zoom microscope.
If it leaked through the anti-shock you always easily can feel that the
anti-shock is no longer moving smoothly - apart from the fact you
managed to get solid dirt in there.

Therefore you have to strongly consider some maleficent user in your
facility.

In about 17 years of running four different imaging facilities, with
probably more than a thousand users, I have experienced much worse
deliberately taken action by some weird, disappointed or mistreated by
their PIs users.

That is my unconvenient expertise.

--
Ralf Palmisano
Head - Optical Imaging Centre Erlangen

Hartmannstr. 14
91052 Erlangen, Germany
+49-9131-85-64300 (Office)
+49-9131-85-64301 (Secretary)
+49-9131-85-64302 (Fax)

www.oice.uni-erlangen.de

------------------------------

Date:    Mon, 4 Apr 2016 16:57:25 +0200
From:    Damien Alcor <[hidden email]<mailto:[hidden email]>>
Subject: Water dispenser

*****
To join, leave or search the confocal microscopy listserv, go to:
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=rd6tbv5SNLS6KGsQfZMbxgduGK-Ix0BBEY_r6PUwJPo&e=
Post images on https://urldefense.proofpoint.com/v2/url?u=http-3A__www.imgur.com&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=BZf7qtxmmjSwf5P4BXDgTXACvscuZ4QdiusEUVetdPY&e=  and include the link in your posting.
*****

Hi,

I have a confocal Nikon A1R with the JOB module for multiwell plate
screening. Nikon does not provide a system to feed water (or oil for oil
objective) to maintain the contact using a water immersion objective
(40x WI). Would you know a company that could provide such a device?
Have you developped your own device?

Kind regards

Damien Alcor

--
Damien Alcor
Responsable Plateforme Biophotonique
Bâtiment Universitaire Archimed
INSERM U1065, C3M
151 route Saint Antoine de Ginestière
BP 2 3194
06204 NICE Cedex 3
Phone: (33) 04 89 06 4268/4251/4275
email:[hidden email]<mailto:[hidden email]>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.unice.fr_c3m_index.php_the-2Dfacility_&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=53dHu01utNA9vFzBV4-eohbYhQiEAi54bgEGqB6kZG8&e=

------------------------------

Date:    Sun, 3 Apr 2016 19:55:29 +0000
From:    Lloyd Donaldson <[hidden email]<mailto:[hidden email]>>
Subject: Re: double staining test.

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------------------------------

Date:    Mon, 4 Apr 2016 17:18:19 +0000
From:    "Eric Griffis (Staff)" <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

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MTUwOTYNCg==

------------------------------

Date:    Mon, 4 Apr 2016 19:34:12 +0000
From:    "Cammer, Michael" <[hidden email]<mailto:[hidden email]>>
Subject: Re: Oil on back focal plane of objective

*****
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*****

We have not had a problem with oil getting inside an objective so that it c=
ompromises resolution.  However, it can get on the outside of the inner bar=
rel and run down inside the microscope body where it gets on dichroics, oth=
er glass surfaces, or onto microschips where is destroys them  We've also h=
ad problems with salty water (PBS? Media?) dripping inside the scopes and d=
estroying mechanical parts.
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
Michael Cammer, Microscopy Core & Skirball Institute, NYU Langone Medical =
Center
                      Cell:  914-309-3270     ** Office: Skirball 2nd Floor=
main office, back right **
          https://urldefense.proofpoint.com/v2/url?u=http-3A__ocs.med.nyu.edu_microscopy&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=XUKpaK9twf9mNqXASGJa9L2VXd6QvkT-ahYgsgWTbNs&e=  & https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopynotes.com_&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=aZnAJleUOtE_6lpYHruVno3Uhl-2LU2jOrZLsmpnHr8&e=

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On=
Behalf Of David Claypool
Sent: Monday, April 04, 2016 11:32 AM
To: [hidden email]<mailto:[hidden email]>
Subject: Re: Oil on back focal plane of objective

*****
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in_wa-3FA0-3Dconfocalmicroscopy&d=3DCwIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOBGmuw=
5jHLjgvtN2r4ehE&r=3DoU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=3D0_k5e3G=
-Hn49IB14k9dJGFlZc5DXSvZO_7gzJFinUEk&s=3DUVMjVrVCxdXrHEXBV1-fiyUfB4zfb6efRr=
yqxjLCy08&e=3D
Post images on https://urldefense.proofpoint.com/v2/url?u=3Dhttp-3A__www.im=
gur.com&d=3DCwIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=3DoU_0=
5LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=3D0_k5e3G-Hn49IB14k9dJGFlZc5DXSvZ=
O_7gzJFinUEk&s=3DyfwCDBdHQKzrvPQtmK0Le5aEHd2TxzRDXAi69udTgs8&e=3D  and incl=
ude the link in your posting.
*****

To All,

This is a somewhat common problem with oil immersion objectives on inverted=
microscopes and is not limited to any one brand or manufacturer. =


If excess oil is not removed after using the objective m(a simple wipe with=
lens tissue will suffice), gravity will eventually cause the oil to run do=
wn the spring loaded front lens barrel into the objective. Over time enough=
oil will build up in the objective, so that it can find its way between th=
e inner lens elements.

The other scenario is if the front lens of the objective has been crashed i=
nto a specimen holder or the underside of a x-y stage. This can cause the s=
eal around the front lens to develop a microcrack and the immersion oil wil=
l find its way into the objective.  =


Either of the above damage is usually repairable by the manufacturer, but n=
ot under warranty.

I have seen people use a piece of a pipe cleaner wrapped around the objecti=
ve to catch the excess oil, but you need to remember to change it regularly.

David J. Claypool
Digital Imaging Product Manager
Micro Video Instruments
Office: 800-875-2041 x5221
Cell: 603-809-5342
[hidden email]<mailto:[hidden email]>

On Apr 4, 2016, at 11:16 AM, Zdenek Svindrych <[hidden email]<mailto:[hidden email]>> wrote:
=

*****
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2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=3DCwIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOB
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zfb6efRryqxjLCy08&e=3D Post images on =

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wIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=3DoU_05LztNstAydlbm=
5L5GDu_vAdjXk3frDLx_CqKkuo&m=3D0_k5e3G-Hn49IB14k9dJGFlZc5DXSvZO_7gzJFinUEk&=
s=3DyfwCDBdHQKzrvPQtmK0Le5aEHd2TxzRDXAi69udTgs8&e=3D  and include the link =
in your posting.
*****
=

=

Hi.
To me it seems very unlikely someone would do that on purpose. It's =

more likely that some greenhorn user misunderstood the sentence "don't =

forget to apply oil to the oil immersion objective" or something like =

that in your manual (I'm sure you don't specifically note that it's =

not necessary to unscrew the objective from the turret to do that :-).
But seriously, if the oil got inside the objective through the front =

lens seal and found its way to the last lens, the lens is probably =

full of oil and can't be fixed. You may try to clean it and test =

whether it performs well, or ask the vendor whether they can check it =

for you (and ask how much it would cost!). If you have service =

contract, they might do it for you for free, you might even be covered by=
some sort of warranty.
=

I doubt oil could get there from the lens threads through capillary =

action, and oil is not superfluid...
=

Good Luck!
=

zdenek
=

=

---------- P=F9vodn=ED zpr=E1va ----------
Od: Gary Laevsky <[hidden email]<mailto:[hidden email]>>
Komu: [hidden email]<mailto:[hidden email]>
Datum: 4. 4. 2016 10:39:50
P=F8edm=ECt: Re: Oil on back focal plane of objective
=

"*****
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2Dbin_wa-3FA0-3Dconfocalmicroscopy&d=3DCwIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOB
Gmuw5jHLjgvtN2r4ehE&r=3DoU_05LztNstAydlbm5L5GDu_vAdjXk3frDLx_CqKkuo&m=3D0_
k5e3G-Hn49IB14k9dJGFlZc5DXSvZO_7gzJFinUEk&s=3DUVMjVrVCxdXrHEXBV1-fiyUfB4
zfb6efRryqxjLCy08&e=3D Post images on =

https://urldefense.proofpoint.com/v2/url?u=3Dhttp-3A__www.imgur.com&d=3DC=
wIFBA&c=3Dj5oPpO0eBH1iio48DtsedbOBGmuw5jHLjgvtN2r4ehE&r=3DoU_05LztNstAydlbm=
5L5GDu_vAdjXk3frDLx_CqKkuo&m=3D0_k5e3G-Hn49IB14k9dJGFlZc5DXSvZO_7gzJFinUEk&=
s=3DyfwCDBdHQKzrvPQtmK0Le5aEHd2TxzRDXAi69udTgs8&e=3D  and include the link =
in your posting.
*****
=

Hi All,
=

To be clear, if oil was leaking down the barrel of the objective, and =

ended up on the center of the back focal plan, there would be a trail =

from the edge, right?
=

This was a solitary drop on the center of the back.
=

I'm trying to find someone that will correct me from thinking this was =

a malicious act.
=

Best,
=

gary
=

On Mon, Apr 4, 2016 at 5:48 AM, Gary Laevsky =

<[hidden email]<mailto:[hidden email]>>
wrote:
=

Hi All,
=

Trying not to be cynical here, but, can anybody please explain to me =

if there is a way for a drop of oil to end up on the center of the =

back focal plane of an objective (oil immersion objective) on an =

inverted scope. No smear from the side.
=

Thanks.
=

--
Best,
=

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310
=

=

=

--
Best,
=

Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Dept. of Molecular Biology
Washington Rd.
Princeton University
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310"

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=3D=3D=3D=3D=3D=3D=3D=3D

------------------------------

Date:    Mon, 4 Apr 2016 23:06:34 +0200
From:    Damien Alcor <[hidden email]<mailto:[hidden email]>>
Subject: Re: Water dispenser

*****
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*****

Hi Bertrand,

Actually, I already have such a cap which I bought from Sutter (through
Nikon). The problem is the control of the amount of water not to “flood”
the microscope! They only provided a pump to infuse water. It seems
difficult to me to control the amount of water while sampling multiple
positions or long time lapse.

I guess a system infusion/withdrawal would be necessary to equilibrate
the amount of water on the lens (the cap is not suited for that). Or a
system to collect the excess of water by gravity?

Have you already planned something?

Best,

Damien



Le 04/04/2016 22:31, Bertrand Vernay a écrit :
Hi Damien

I'm in touch with Sutter in the States. See below. I'm thinking of ordering one cap from them for our Nikon water 1.15 x40.

Bertrand


-----------------------------------------------------------
From: Chris Ballard [mailto:[hidden email]]
Sent: 16 March 2016 17:35
To: [hidden email]<mailto:[hidden email]>
Subject: Re: Fwd: Brass caps for Nikon objectives

Hi Bertand,

Good timing!   I am making one for that exact lens for a customer in Italy right now.  In fact, we usually require that the objective in question is shipped to Sutter because the tolerance as so close.  I currently have this objective on my desk and I will be designing over the next couple of weeks.

We now Nickel coat these parts.  (see attached)

Would you like me to send you a quote once I figure out how much each one is going to cost?  Usually they are around ~$950 but I will have a precise cost soon.

Best regards,
Chris


--
Bertrand Vernay, PhD
Imaging Facility Manager
Website: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.crm.ed.ac.uk_facilities_imaging&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=EPAqUqLZPEEHRJ6bcS_9myRtwaYlfyFidK_pcQk6jsk&e=
MRC Centre for Regenerative Medicine
SCRM Building
The University of Edinburgh
Edinburgh bioQuarter
5 Little France Drive
Edinburgh
EH16 4UU

Office: +0044 (0)131 651 9543




--
Damien Alcor
Responsable Plateforme Biophotonique
Bâtiment Universitaire Archimed
INSERM U1065, C3M
151 route Saint Antoine de Ginestière
BP 2 3194
06204 NICE Cedex 3
Phone: (33) 04 89 06 4268/4251/4275
email:[hidden email]<mailto:[hidden email]>
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.unice.fr_c3m_index.php_the-2Dfacility_&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=53dHu01utNA9vFzBV4-eohbYhQiEAi54bgEGqB6kZG8&e=

------------------------------

Date:    Mon, 4 Apr 2016 15:19:18 -0600
From:    Craig Brideau <[hidden email]<mailto:[hidden email]>>
Subject: Re: Water dispenser

*****
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*****

Maybe hook it up to a syringe pump? Some of these types of pumps can be
rigged to advance the plunger a fixed amount on receipt of a triggering
pulse. This would let you 'fire' the syringe a fixed amount every time you
change position.

Craig

On Mon, Apr 4, 2016 at 3:06 PM, Damien Alcor <[hidden email]<mailto:[hidden email]>> wrote:

*****
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*****

Hi Bertrand,

Actually, I already have such a cap which I bought from Sutter (through
Nikon). The problem is the control of the amount of water not to =E2=80=
=9Cflood=E2=80=9D
the microscope! They only provided a pump to infuse water. It seems
difficult to me to control the amount of water while sampling multiple
positions or long time lapse.

I guess a system infusion/withdrawal would be necessary to equilibrate th=
e
amount of water on the lens (the cap is not suited for that). Or a system
to collect the excess of water by gravity?

Have you already planned something?

Best,

Damien



Le 04/04/2016 22:31, Bertrand Vernay a =C3=A9crit :

Hi Damien

I'm in touch with Sutter in the States. See below. I'm thinking of
ordering one cap from them for our Nikon water 1.15 x40.

Bertrand


-----------------------------------------------------------
From: Chris Ballard [mailto:[hidden email]]
Sent: 16 March 2016 17:35
To: [hidden email]<mailto:[hidden email]>
Subject: Re: Fwd: Brass caps for Nikon objectives

Hi Bertand,

Good timing!   I am making one for that exact lens for a customer in
Italy right now.  In fact, we usually require that the objective in
question is shipped to Sutter because the tolerance as so close.  I
currently have this objective on my desk and I will be designing over th=
e
next couple of weeks.

We now Nickel coat these parts.  (see attached)

Would you like me to send you a quote once I figure out how much each on=
e
is going to cost?  Usually they are around ~$950 but I will have a preci=
se
cost soon.

Best regards,
Chris


--
Bertrand Vernay, PhD
Imaging Facility Manager
Website: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.crm.ed.ac.uk_facilities_imaging&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=EPAqUqLZPEEHRJ6bcS_9myRtwaYlfyFidK_pcQk6jsk&e=
MRC Centre for Regenerative Medicine
SCRM Building
The University of Edinburgh
Edinburgh bioQuarter
5 Little France Drive
Edinburgh
EH16 4UU

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------------------------------

Date:    Mon, 4 Apr 2016 18:39:49 -0300
From:    jens rietdorf <[hidden email]<mailto:[hidden email]>>
Subject: laser alignment issue FluoView1000

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Listers,

following the installation of a new air condition, we are not succeeding to
get any light after the scanhead of our Olympus FluoView1000 CLSM. End of
fiber, there is plenty of light. Behind the scanhead, no light arrives. We
work with an inverted IX 81 microscope.

Unfortunately the service situation in Brazil is very poor, so if there
were any suggestions how to proceed, I would rather try to check the
alignment myself.

Anyone having experience with the FluoView1000 scanhead?

Thank you, Jens

Dr. Jens Rietdorf, visiting scientist @ center for technological
development in health CDTS, Oswaldo Cruz Foundation Fiocruz, Rio de Janeiro
Brasil.

------------------------------

Date:    Mon, 4 Apr 2016 12:57:05 -0700
From:    Kurt Thorn <[hidden email]<mailto:[hidden email]>>
Subject: Re: Water dispenser

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On 4/4/2016 7:57 AM, Damien Alcor wrote:
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Hi,

I have a confocal Nikon A1R with the JOB module for multiwell plate
screening. Nikon does not provide a system to feed water (or oil for
oil objective) to maintain the contact using a water immersion
objective (40x WI). Would you know a company that could provide such a
device? Have you developped your own device?

Kind regards

Damien Alcor

Sutter Instruments (https://urldefense.proofpoint.com/v2/url?u=http-3A__sutter.com_&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=DkAnTq3bbJsJ92uRyIIyzzqEVTfWppqF-8vMrEc0IWU&e= ) can machine a collar with a
needle for water dispensing, that you can then mate to a syringe pump.
We got partway into setting up such a system for a 40x WI objective, but
the project that needed it ended, so we never got it completely working.

Kurt

--
Kurt Thorn
Associate Professor
Director, Nikon Imaging Center
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------------------------------

Date:    Mon, 4 Apr 2016 22:40:49 +0000
From:    "Knecht, David" <[hidden email]<mailto:[hidden email]>>
Subject: Immersion oil

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*****

If you are imaging a sample in aqueous media through glass with a 1.25 NA 1=
00x oil objective, what is the correct refractive index immersion oil to us=
e?  You are going to have refractive index mismatches no matter what, but a=
re some more critical than others?  Is the 1.25 NA objective designed to be=
used with 1.515 RI oil or should one use a lower RI oil or match the sampl=
e by using water?  Dave

Dr. David Knecht
Professor of Molecular and Cell Biology
Core Microscopy Facility Director
University of Connecticut
91 N. Eagleville Rd.
Storrs, CT 06269
860-486-2200

------------------------------

Date:    Mon, 4 Apr 2016 19:57:33 -0600
From:    Craig Brideau <[hidden email]<mailto:[hidden email]>>
Subject: Re: Immersion oil

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*****

Oil objectives always assume you are trying to match to the index of the
coverslip. By using an oil with the same index as the coverslip, you are
essentially removing the coverslip first surface from the light path. That
is, you have a constant medium between the end of your objective and the
inner surface of the coverslip. This assumes your sample is resting
perfectly against the coverslip surface though, so reality varies. If you
have a gap between that inner surface and the sample you now have a layer
of mounting media to get through, with its own index of refraction
difference.

Craig

On Mon, Apr 4, 2016 at 4:40 PM, Knecht, David <[hidden email]<mailto:[hidden email]>>
wrote:

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*****

If you are imaging a sample in aqueous media through glass with a 1.25 NA
100x oil objective, what is the correct refractive index immersion oil to
use?  You are going to have refractive index mismatches no matter what, but
are some more critical than others?  Is the 1.25 NA objective designed to
be used with 1.515 RI oil or should one use a lower RI oil or match the
sample by using water?  Dave

Dr. David Knecht
Professor of Molecular and Cell Biology
Core Microscopy Facility Director
University of Connecticut
91 N. Eagleville Rd.
Storrs, CT 06269
860-486-2200


------------------------------

Date:    Tue, 5 Apr 2016 03:40:47 +0000
From:    Jeremy Adler <[hidden email]<mailto:[hidden email]>>
Subject: Re: Immersion oil

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*****

An 'oil' objective requires a medium with the RI of oil between itself and =
the coverslip. This provides the RI the objective is designed for until the=
outer edge of the coverslip. The RI matching should continue beyond the co=
verslip, and   from here it goes wrong for a water based sample. The degrad=
ation increases with the distance from the coverslip to the imaging plane. =
So if you are interested in how cells desperately cling to coverslips then =
the mismatch in RIs is not too serious. =0A=
=0A=
But given the time and resources invested in an experiment why not optimize=
the imaging by using a water objective.=0A=
=0A=
The question can be addressed experimentally and most manufacturers will le=
nd you an objective in the hope that you will be impressed by its performan=
ce. An appropriate test sample would be a slide with 200nm fluorescent micr=
ospheres on both the coverslip and slide with a spacer between them whose t=
hickness matches the location of your imaging plane. Compare the observed P=
SFs of the microspheres on the coverslip with the PSFs on the slide and you=
have an empirical answer. =0A=
=0A=
=0A=
=0A=
=0A=
________________________________________=0A=
From: Confocal Microscopy List [[hidden email]<mailto:[hidden email]>] on behalf=
of Knecht, David [[hidden email]<mailto:[hidden email]>]=0A=
Sent: 05 April 2016 00:40=0A=
To: [hidden email]=0A=<mailto:[hidden email]=0A=>
Subject: Immersion oil=0A=
=0A=
*****=0A=
To join, leave or search the confocal microscopy listserv, go to:=0A=
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.umn.edu_cgi-2Dbin_wa-3FA0-3D3Dconfocalmicroscopy-3D0A-3D&d=CwIBaQ&c=lb62iw4YL4RFalcE2hQUQealT9-RXrryqt9KZX2qu2s&r=ZfCvkQ9x54SB55UhnhhE2u1SIrroLLHN52nAEGv4nww&m=xXQvklLCfV7tPNSlq3x01KPyJmkkvAF3TO5WU78pzgA&s=WRm88MaBRfRPMlvgBBIJqOjT7jAGSxqUAXpPJJsNboc&e=
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=0A=
*****=0A=
=0A=
If you are imaging a sample in aqueous media through glass with a 1.25 NA 1=
00x oil objective, what is the correct refractive index immersion oil to us=
e?  You are going to have refractive index mismatches no matter what, but a=
re some more critical than others?  Is the 1.25 NA objective designed to be=
used with 1.515 RI oil or should one use a lower RI oil or match the sampl=
e by using water?  Dave=0A=
=0A=
Dr. David Knecht=0A=
Professor of Molecular and Cell Biology=0A=
Core Microscopy Facility Director=0A=
University of Connecticut=0A=
91 N. Eagleville Rd.=0A=
Storrs, CT 06269=0A=
860-486-2200=

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