Re: CONFOCALMICROSCOPY Digest - 2 Oct 2016 to 3 Oct 2016 (#2016-93)
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Sandra Ritz |
Re: CONFOCALMICROSCOPY Digest - 2 Oct 2016 to 3 Oct 2016 (#2016-93)
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Philippe, This publication might be interesting for you. Advanced light microscopy core facilities: Balancing service, science and career (published June 2016). http://onlinelibrary.wiley.com/doi/10.1002/jemt.22648/abstract Best, Sandra Dr. Sandra Ritz ------------------------------------------------------- Head of Microscopy Core Facility Institute of Molecular Biology gGmbH (IMB) Ackermannweg 4 55128 Mainz Germany Phone: +49 6131 39 21 568 [hidden email] www.imb-mainz.de -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of CONFOCALMICROSCOPY automatic digest system Sent: Tuesday, October 4, 2016 7:00 AM To: [hidden email] Subject: CONFOCALMICROSCOPY Digest - 2 Oct 2016 to 3 Oct 2016 (#2016-93) There are 11 messages totaling 2195 lines in this issue. Topics of the day: 1. Ludl control box 2. 3rd party microscopy engineers (UK) 3. {SPAM?} 3rd party microscopy engineers (UK) 4. Planning for imaging facility 5. Deposit FluoSpheres (carboxylate-modified) for calibration measurements 6. Opinions on different objective heaters? (3) 7. Photonics Instruments Inc Mosaic Digital Diaphragm 8. intracellular ion probes **Vendor Reply** 9. Please send me names and web links for biomedical light microscopy companies and products I do not already have listed ---------------------------------------------------------------------- Date: Mon, 3 Oct 2016 05:01:31 -0500 From: Edward Ward <[hidden email]> Subject: Ludl control box ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, We've recently inherited, among other things, an Ludle biopoint x-y stage however the ludl control box we currently have (identical to the one in this listing http://www.ebay.com/itm/LUDL-ELECTRONIC-LEP-BIP-POINT-MICROSCOPE-X-Y-Z-STAGE-AND-CONTROL-CONTROLLER-/261950930770) does not contain the necessary drive modules but nicely controls the rest of our setup. Does anyone know where we could get the drive modules to go in the box or have one not being used? We've also acquired a lot of spare equipment including components for a PALM micro-dissection system so would be very open to trading parts if that tempts anyone? Cheers, Edward ------------------------------ Date: Mon, 3 Oct 2016 12:57:44 +0000 From: "Minogue, Shane" <[hidden email]> Subject: 3rd party microscopy engineers (UK) ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi List, I'm looking for a good engineer to service our ageing LSM 510 Meta. Can anyone recommend someone that covers London? Shane From: "SANCHEZ MARTIN, CARLOS" <[hidden email]<mailto:[hidden email]>> Reply-To: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> Date: Sun, 2 Oct 2016 08:01:27 +0200 To: <[hidden email]<mailto:[hidden email]>> Subject: Re: Planning for imaging facility ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, Philippe, Although a bit out of date (2011), this article may help you: http://onlinelibrary.wiley.com/doi/10.1002/0471142956.cy1222s57/abstract Goog luck. Carlos George McNamara <[hidden email]<mailto:[hidden email]>> escribió: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Philippe, Thanks for the plug. I hope I did not contradict my 2007 self too much. I just added this to my MyNCBI bibliography. It is my 42 citation - and as some of you know, 42 is a very special number. for anyone who does not, see https://en.wikipedia.org/wiki/42_(number) -- "popular culture" section. http://www.labmanager.com/business-management/2007/03/bio-medical-light-microscopy-imaging-facility-management PDFs of the magazine are at http://www.labmanager.com/magazine (subscription may be required?). McNamara G, Boswell CA 2007 Bio-Medical Light Microscopy Imaging Facility Management. Lab Manager 2(3): 11-15. http://photos.labmanager.com/magazinePDFs/archives/labmanager200703-dl.pdf While looking up the PDF link, I found what will likely be a much more useful article for everyone: Collins 2007 HUMOR IN LABORATORY MANAGEMENT (SERIOUSLY!). Lab Manager 2(5): 13-16. http://photos.labmanager.com/magazinePDFs/archives/labmanager200705-dl.pdf // Finally, it is now October, so time for the 2016 edition of The Halloween Game. Please go to https://www.linkedin.com/pulse/halloween-2016-trick-treat-can-you-self-identify-find-george-mcnamara and self-identify AND see if you can find matches for Guy Cox, Jim Pawley, and (at the risk of my being banned from the listserv) Martin Wessendorf. Images of each (hopefully from this decade) are available at, http://www.leica-microsystems.com/science-lab/authors/detail/author/cox-1/ https://ca.linkedin.com/in/james-pawley-590b6019 http://www.neuroscience.umn.edu/ProStu/facprof/wessendo.html enjoy, George p.s. Guy, Jim and Martin are welcome to try to identify me, https://www.linkedin.com/in/georgemcnamara On 10/1/2016 2:31 PM, phil laissue wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Christophe, two older and very useful publications, in case you don't have them already: https://www.researchgate.net/publication/226531231_Design_and_Function_of_a_Light-Microscopy_Facility http://www.gum2012.fionastoreydesign.co.uk/Downloads/BioImaging%20Facility%20Management%20-%20McNamara.pdf Cheers, Philippe _________________________________________ Philippe Laissue, PhD Royal Society Industry Fellow School of Biological Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK (0044) 01206 872246 / (0044) 07842 676 456 [hidden email]<mailto:[hidden email]> privatewww.essex.ac.uk/~plaissue<http://privatewww.essex.ac.uk/%7Eplaissue> On 1 October 2016 at 10:14, Christophe Leterrier <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, Do you know a good resource for planning an imaging facility in terms of building and related equipment: AC, power outlets, lighting? Any important point the collective wisdom of the confocal mailing list could add to this list? Thanks, Christophe -- Christophe Leterrier Researcher Axonal Domains Architecture Team CRN2M CNRS UMR 7286 Aix Marseille University, France -- George McNamara, PhD Houston, TX 77054 [hidden email]<mailto:[hidden email]>https://www.linkedin.com/in/georgemcnamarahttps://works.bepress.com/gmcnamara/75/http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 Carlos Sánchez Martín Servicio de Microscopía Óptica y Confocal (SMOC) (Lab. 310) (Optical and Confocal Microscopy Facility) Centro de Biología Molecular Severo Ochoa (UAM-CSIC) C/Nicolás Cabrera, 1. Universidad Autónoma de Madrid Cantoblanco. E-28049. Madrid. Spain Tlf. 34-91 196 4613/4643 Fax. 34-91 196 4420 E-mail: [hidden email]/[hidden email]<mailto:[hidden email]/[hidden email]> Blog: http://csanchezmad.wordpress.com<http://csanchezmad.wordpress.com/> Web: http://www.cbm.csic.es/confocal Red Española de Microscopía Óptica Avanzada (REMOA) (Spanish Network of Advanced Optical Microscopy) http://remoa.wikispaces.com<http://remoa.wikispaces.com/> http://remoa.net<http://remoa.net/> Plataforma de Microscopía para Biociencias Red de laboratorios de la Comunidad de Madrid http://www.madrimasd.org/Laboratorios/plataformas-red/ficha.asp?IdPreli=3 ------------------------------ Date: Mon, 3 Oct 2016 13:48:42 +0000 From: "Moulding, Dale" <[hidden email]> Subject: Re: {SPAM?} 3rd party microscopy engineers (UK) ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Shane, I've heard good things about Kevin Champion at http://ktecmicroscopes.co.uk/ cheers Dale ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Minogue, Shane <[hidden email]> Sent: 03 October 2016 13:57 To: [hidden email] Subject: {SPAM?} 3rd party microscopy engineers (UK) ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi List, I'm looking for a good engineer to service our ageing LSM 510 Meta. Can anyone recommend someone that covers London? Shane From: "SANCHEZ MARTIN, CARLOS" <[hidden email]<mailto:[hidden email]>> Reply-To: Confocal Microscopy List <[hidden email]<mailto:[hidden email]>> Date: Sun, 2 Oct 2016 08:01:27 +0200 To: <[hidden email]<mailto:[hidden email]>> Subject: Re: Planning for imaging facility ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hello, Philippe, Although a bit out of date (2011), this article may help you: http://onlinelibrary.wiley.com/doi/10.1002/0471142956.cy1222s57/abstract Goog luck. Carlos George McNamara <[hidden email]<mailto:[hidden email]>> escribió: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Philippe, Thanks for the plug. I hope I did not contradict my 2007 self too much. I just added this to my MyNCBI bibliography. It is my 42 citation - and as some of you know, 42 is a very special number. for anyone who does not, see https://en.wikipedia.org/wiki/42_(number) -- "popular culture" section. http://www.labmanager.com/business-management/2007/03/bio-medical-light-microscopy-imaging-facility-management PDFs of the magazine are at http://www.labmanager.com/magazine (subscription may be required?). McNamara G, Boswell CA 2007 Bio-Medical Light Microscopy Imaging Facility Management. Lab Manager 2(3): 11-15. http://photos.labmanager.com/magazinePDFs/archives/labmanager200703-dl.pdf While looking up the PDF link, I found what will likely be a much more useful article for everyone: Collins 2007 HUMOR IN LABORATORY MANAGEMENT (SERIOUSLY!). Lab Manager 2(5): 13-16. http://photos.labmanager.com/magazinePDFs/archives/labmanager200705-dl.pdf // Finally, it is now October, so time for the 2016 edition of The Halloween Game. Please go to https://www.linkedin.com/pulse/halloween-2016-trick-treat-can-you-self-identify-find-george-mcnamara and self-identify AND see if you can find matches for Guy Cox, Jim Pawley, and (at the risk of my being banned from the listserv) Martin Wessendorf. Images of each (hopefully from this decade) are available at, http://www.leica-microsystems.com/science-lab/authors/detail/author/cox-1/ https://ca.linkedin.com/in/james-pawley-590b6019 http://www.neuroscience.umn.edu/ProStu/facprof/wessendo.html enjoy, George p.s. Guy, Jim and Martin are welcome to try to identify me, https://www.linkedin.com/in/georgemcnamara On 10/1/2016 2:31 PM, phil laissue wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Christophe, two older and very useful publications, in case you don't have them already: https://www.researchgate.net/publication/226531231_Design_and_Function_of_a_Light-Microscopy_Facility http://www.gum2012.fionastoreydesign.co.uk/Downloads/BioImaging%20Facility%20Management%20-%20McNamara.pdf Cheers, Philippe _________________________________________ Philippe Laissue, PhD Royal Society Industry Fellow School of Biological Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK (0044) 01206 872246 / (0044) 07842 676 456 [hidden email]<mailto:[hidden email]> privatewww.essex.ac.uk/~plaissue<http://privatewww.essex.ac.uk/%7Eplaissue> On 1 October 2016 at 10:14, Christophe Leterrier <[hidden email]<mailto:[hidden email]>> wrote: ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi, Do you know a good resource for planning an imaging facility in terms of building and related equipment: AC, power outlets, lighting? Any important point the collective wisdom of the confocal mailing list could add to this list? Thanks, Christophe -- Christophe Leterrier Researcher Axonal Domains Architecture Team CRN2M CNRS UMR 7286 Aix Marseille University, France -- George McNamara, PhD Houston, TX 77054 [hidden email]<mailto:[hidden email]>https://www.linkedin.com/in/georgemcnamarahttps://works.bepress.com/gmcnamara/75/http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 Carlos Sánchez Martín Servicio de Microscopía Óptica y Confocal (SMOC) (Lab. 310) (Optical and Confocal Microscopy Facility) Centro de Biología Molecular Severo Ochoa (UAM-CSIC) C/Nicolás Cabrera, 1. Universidad Autónoma de Madrid Cantoblanco. E-28049. Madrid. Spain Tlf. 34-91 196 4613/4643 Fax. 34-91 196 4420 E-mail: [hidden email]/[hidden email]<mailto:[hidden email]/[hidden email]> Blog: http://csanchezmad.wordpress.com<http://csanchezmad.wordpress.com/> Web: http://www.cbm.csic.es/confocal Red Española de Microscopía Óptica Avanzada (REMOA) (Spanish Network of Advanced Optical Microscopy) http://remoa.wikispaces.com<http://remoa.wikispaces.com/> http://remoa.net<http://remoa.net/> Plataforma de Microscopía para Biociencias Red de laboratorios de la Comunidad de Madrid http://www.madrimasd.org/Laboratorios/plataformas-red/ficha.asp?IdPreli=3 ------------------------------ Date: Mon, 3 Oct 2016 16:14:43 +0000 From: Kathryn Spencer <[hidden email]> Subject: Re: Planning for imaging facility ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We like the duct socks for minimizing air currents. http://www.ductsox.com/why-fabric?utm_source=bing&utm_medium=cpc&utm_campaign=DuctSox&utm_term=ductsox&utm_content=Duct%20Sox Kathy The Scripps Research Institute -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Phillips, Thomas E. Sent: Saturday, October 01, 2016 7:06 AM To: [hidden email] Subject: Re: Planning for imaging facility ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** If only I was as well funded as George and some of the rest of you. We muddle through on a much tighter budget but have lots of toys. My number one rec is to ask Leica and Zeiss for their detailed specification sheets for the room. They nicely spell out the electrical and ventilation characteristics. It is hard to have too many separate electrical lines - a mix of 120 and 220 is nice. Don't forget the heat output of the line conditioners/backup power supplies when calculating heat load for AC. Having a diffuser over air vent is nice. Give yourself plenty of space around the anti-vibration table on all sides so you can have access to the instruments front and back. Get a dimmer for your lights. Locked cabinets for your spare parts. Thomas E. Phillips, Ph.D Curator’s Distinguished Teaching Professor of Biological Sciences Director, Molecular Cytology Core 2 Tucker Hall University of Missouri Columbia, MO 65211-7400 573-882-4712 (office) 573-882-0123 (fax) [hidden email] http://www.biology.missouri.edu/faculty/phillips.html http://microscopy.missouri.edu -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of George McNamara Sent: Saturday, October 1, 2016 8:35 AM To: [hidden email] Subject: Re: Planning for imaging facility ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Christophe, Here's my bullet list: * lasers ... Bob Chow at USC had one big laser with fiber optics delivering light to each microscope in different parts of his lab. I'm not suggesting you buy one 3.5 W Arctic laser (http://www.wickedlasers.com/arctic ... do not look at laser with remaining eye") and have it drive all your scopes, but may be useful to have a laser stack. Here is a referene for OCTOPUS: Optics clustered to output unique solutions: a multi-laser facility for combined single molecule and ensemble microscopy. Clarke DT, Botchway SW, Coles BC, Needham SR, Roberts SK, Rolfe DJ, Tynan CJ, Ward AD, Webb SE, Yadav R, Zanetti-Domingues L, Martin-Fernandez ML. Rev Sci Instrum. 2011 Sep;82(9):093705. doi: 10.1063/1.3635536. PMID: 21974592 Clarke's Figure 1 is a layout of their facility - you can ask them for more details than in the paper. * A/C: HEPA filters, no drafts on the microscopes (or users). Bonus tip: avoid buildings whose A/C ducting includes right angle turns - makes the building noisy (and likely energy inefficient). *Electrical: (i) lots of power outlets. (ii) every station should be using an uninterruptible power supply (UPS). Buy UPS's with more power and outlets than you think you'll need. Most power outages are a few seconds, but every building I've working in for the past 20 years has had extended power outages. Sometimes the "red outlets" (hospital essential equipment) power has gone out. * Fast Ethernet ports ... lots of them. Bluetooth may be nice for listening to your new iPhone, but wired is always going to be faster than wireless for data transfer (at same cost). * Network infrastructure ... try to get leading edge (100 Gbit?) installed. * You may want or need major compute power at the acquisition station and/or close by. Examples: -- dual (or triple) NVidia GTX 1080 or new TITAN GPU cards (~10 Gigaflops each) per acquisition PC. ... Microvolution or SVI Huygens "instant gratification" deconvolution -- Acquifer (https://www.acquifer.de/) Hive or similar data processing and storage. -- short term local high performance storage ... and fast network connection and software control to move (week?) old data to cheaper I.T. storage. * space for big, high resolution, monitors, for every acquisition and analysis workstation (even if you also have VR/AR), for example https://www.amazon.com/YAMAKASI-O40USUT-Inch-Monitor-10Bit/dp/B017VWX3U8 (see my August 2, 2016 comment on what port to use and possible cable and adapters). * vibrations: avoid being near elevators, other heavy equipment, especially stuff on the roof (avoid top floor, even though penthouse microscope suite sounds sweet for the views). * microscopes should be in rooms without windows. Up to you to have windows or not ... in the future maybe you'll be wearing VR/AR headset instead of dealing with "no glare" monitor screens. Sept 9 arivis imaging ad by Michael Wussow mentions, "Experience and knowledge of the Virtual Reality / Augmented Reality market and products" (I applied yesterday). See also online content by googling for: arivis zeiss ... for example, https://3dprint.com/143825/immersive-microscopy * avoid basement or first floor ... flooding, thefts from outsiders. On 10/1/2016 4:14 AM, Christophe Leterrier wrote: > ***** To join, leave or search the confocal microscopy listserv, go > to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images > on http://www.imgur.com and include the link in your posting. ***** > Hi, > > Do you know a good resource for planning an imaging facility in terms > of building and related equipment: AC, power outlets, lighting? Any > important point the collective wisdom of the confocal mailing list > could add to this list? > > Thanks, > > Christophe > > -- > Christophe Leterrier > Researcher > Axonal Domains Architecture Team > CRN2M CNRS UMR 7286 > Aix Marseille University, France > -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 ------------------------------ Date: Mon, 3 Oct 2016 16:22:38 +0000 From: WHEELER Ann <[hidden email]> Subject: Re: Deposit FluoSpheres (carboxylate-modified) for calibration measurements ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** The University of Edinburgh is a charitable body, registered in Scotland, with registration number SC005336. ------------------------------ Date: Mon, 3 Oct 2016 16:20:55 -0400 From: Alison North <[hidden email]> Subject: Opinions on different objective heaters? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, The collar on our well-loved 20-20 Tech objective heater for our multiphoton system has finally broken (the wires are coming out). We can't locate the company online any more but would be delighted to hear if it has been bought by somebody else so that we could get a replacement? Otherwise I would be grateful for people's comments on their favourite objective heaters, bearing in mind that the Olympus 25x multiphoton objective we use is (a) fat and (b) costs $30,000, and we are using it in a multi-user facility. I know this topic was discussed a few years ago, but it may be that there are new players in the field. Please note that I do NOT wish to hear a commercial plug from the vendors themselves (unless it's from somebody selling the 20-20 heaters!), I want to hear the views of the people who are using them. Thanks so much! Alison -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 ------------------------------ Date: Mon, 3 Oct 2016 21:05:58 +0000 From: "MODEL, MICHAEL" <[hidden email]> Subject: Re: Opinions on different objective heaters? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Alison, I don't know about your particular objective, but Bioptechs heater works Mike -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Alison North Sent: Monday, October 03, 2016 4:21 PM To: [hidden email] Subject: Opinions on different objective heaters? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, The collar on our well-loved 20-20 Tech objective heater for our multiphoton system has finally broken (the wires are coming out). We can't locate the company online any more but would be delighted to hear if it has been bought by somebody else so that we could get a replacement? Otherwise I would be grateful for people's comments on their favourite objective heaters, bearing in mind that the Olympus 25x multiphoton objective we use is (a) fat and (b) costs $30,000, and we are using it in a multi-user facility. I know this topic was discussed a few years ago, but it may be that there are new players in the field. Please note that I do NOT wish to hear a commercial plug from the vendors themselves (unless it's from somebody selling the 20-20 heaters!), I want to hear the views of the people who are using them. Thanks so much! Alison -- Alison J. North, Ph.D., Research Associate Professor and Senior Director of the Bio-Imaging Resource Center, The Rockefeller University, 1230 York Avenue, New York, NY 10065. Tel: office ++ 212 327 7488 Tel: lab ++ 212 327 7486 Fax: ++ 212 327 7489 ------------------------------ Date: Mon, 3 Oct 2016 21:13:51 +0000 From: "Anthony, Neil" <[hidden email]> Subject: Photonics Instruments Inc Mosaic Digital Diaphragm ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi all, I was wondering if anybody has pointers to info sources for connecting to a Photonics Instruments Inc Mosaic Digital Diaphragm. I thought I'd write something to connect it to micro-manger if I can find the low level information. Any info much appreciated. Thanks Neil ________________________________ This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the ------------------------------ Date: Mon, 3 Oct 2016 21:55:59 +0000 From: "Kilgore, Jason A." <[hidden email]> Subject: Re: intracellular ion probes **Vendor Reply** ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ** Vendor Reply ** Posted on behalf of my esteemed colleague, Nick Dolman, here at Thermo Fisher Scientific: The following two papers from the Svoboda lab talk a little about this with respect to calcium indicators. Maravall et al 2000 Biophys J (https://www.ncbi.nlm.nih.gov/pubmed/10777761) Yasuda et al 2004 Sci STKE (https://www.ncbi.nlm.nih.gov/pubmed/14872098) They also cite these additional studies Tsien & Pozzan (https://www.ncbi.nlm.nih.gov/pubmed/2747529) Harkins et al 1993 (https://www.ncbi.nlm.nih.gov/pubmed/8218910) O'Malley et al 1999 (https://www.ncbi.nlm.nih.gov/pubmed/10231797) Busa et al 1992 (https://www.ncbi.nlm.nih.gov/pubmed/1623501) Cheers, Nick Nick J Dolman Ph.D. Senior Staff Scientist Research & Development Thermo Fisher Scientific 100 Technology Drive | Pittsburgh, PA 15219, USA [hidden email]<mailto:[hidden email]> From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of MODEL, MICHAEL Sent: Tuesday, September 27, 2016 5:45 PM To: [hidden email] Subject: intracellular ion probes ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear All, Intracellular ion probes behave differently in the cytoplasm than in the buffer - that is well known. I recall that I read somewhere that this difference is because of viscosity or because of interactions with proteins or maybe because of something else - I got an impression that someone has studied it. But I don't remember where I saw this and cannot think of the right keyword to find the information again. I would be very thankful if someone points me in the right direction. Mike Model ------------------------------ Date: Mon, 3 Oct 2016 17:57:15 -0400 From: Gary Laevsky <[hidden email]> Subject: Re: Opinions on different objective heaters? ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We have Bioptechs as well. Very happy with it for a long time. Best, Gary > On Oct 3, 2016, at 5:05 PM, MODEL, MICHAEL <[hidden email]> wrote: > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Alison, > I don't know about your particular objective, but Bioptechs heater > works > > Mike > > -----Original Message----- > From: Confocal Microscopy List > [mailto:[hidden email]] On Behalf Of Alison North > Sent: Monday, October 03, 2016 4:21 PM > To: [hidden email] > Subject: Opinions on different objective heaters? > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi all, > > The collar on our well-loved 20-20 Tech objective heater for our multiphoton system has finally broken (the wires are coming out). We can't locate the company online any more but would be delighted to hear if it has been bought by somebody else so that we could get a > replacement? Otherwise I would be grateful for people's comments on > their favourite objective heaters, bearing in mind that the Olympus 25x multiphoton objective we use is (a) fat and (b) costs $30,000, and we are using it in a multi-user facility. I know this topic was discussed a few years ago, but it may be that there are new players in the field. > Please note that I do NOT wish to hear a commercial plug from the vendors themselves (unless it's from somebody selling the 20-20 heaters!), I want to hear the views of the people who are using them. > > Thanks so much! > > Alison > > -- > Alison J. North, Ph.D., > Research Associate Professor and > Senior Director of the Bio-Imaging Resource Center, The Rockefeller > University, > 1230 York Avenue, > New York, > NY 10065. > Tel: office ++ 212 327 7488 > Tel: lab ++ 212 327 7486 > Fax: ++ 212 327 7489 ------------------------------ Date: Mon, 3 Oct 2016 17:13:13 -0500 From: George McNamara <[hidden email]> Subject: Please send me names and web links for biomedical light microscopy companies and products I do not already have listed ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear confocal listserv members, Please send me names and web links for biomedical light microscopy companies and products I do not already have listed in the PDF downloadable from https://works.bepress.com/gmcnamara/77/ i would like to send the manuscript Wed October 5, 2016, evening. Please send me the names and web links DIRECTLY, not as a reply to the listserv. I am happy to post the final tables later. Starting on page 20 is a list of a few researcher labs - if you have a lab web site, or know of a lab with a terrific web site, involving biomedical light microscopy and/or image analysis please send it to me -- after checking that is it not already in the PDF (yes, Molecular Expressions and most of the associated vendor sites are in the PDF already). I also posted this request at https://www.linkedin.com/pulse/biomedical-light-microscopy-equipment-reagent-vendors-george-mcnamara so you are welcome to email me through linkedin and/or add a comment to my post. sincerely, George https://www.linkedin.com/in/georgemcnamara -- George McNamara, PhD Houston, TX 77054 [hidden email] https://www.linkedin.com/in/georgemcnamara https://works.bepress.com/gmcnamara/75/ http://www.ncbi.nlm.nih.gov/myncbi/browse/collection/44962650 ------------------------------ End of CONFOCALMICROSCOPY Digest - 2 Oct 2016 to 3 Oct 2016 (#2016-93) ********************************************************************** |
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