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Caroline
If your data are from an SP5 then metadata are stored in the .lei file not in the exported .tif file (metadata are exported together with your images as xml files which you can open in a web browser). I find I can open exported .tif images from LAS AF Lite with almost any imaging programme. Irfanview can batch convert most file types. Picture Publisher and Paint.net are both free and should be able to open these images.
Dr Lloyd Donaldson
Senior Scientist, Project Leader - Microscopy/Wood Identification
Scion - Forests . Products . Innovation
Private Bag 3020, Rotorua
49 Sala Street, Rotorua 3010
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Ph: 64 7 343 5581
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of daniel white
Sent: Sunday, 27 November 2011 3:32 a.m.
To:
[hidden email]
Subject: Re: CONFOCALMICROSCOPY Digest - 24 Nov 2011 to 25 Nov 2011 (#2011-95)
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Hi Caroline,
On Nov 26, 2011, at 7:02 AM, CONFOCALMICROSCOPY automatic digest system wrote:
> Date: Fri, 25 Nov 2011 05:24:51 -0800
> From: bluebug <
[hidden email]>
> Subject: LZW compressed tiff files
>
> Hello All,
> Can anyone recommend a program to decompress LZW tiff files? I exported the
> images from Las AF Lite software for downstream processing in another
> program.
use a program that can read the native leica files so you dont lose the meta data like pixel size.
some progs can open lzw tiff files... and read the leica diles eg:
the Fiji distribution of ImageJ has pretty much everything you will need day to day.
http://fiji.sc Fiji - is just ImageJ (Batteries Included)
Dr. Daniel James White BSc. (Hons.) PhD
Leader - Image Processing Facility,
Senior Microscopist,
Light Microscopy Facility.
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108
01307 DRESDEN
Germany
+49 (0)15114966933 (German Mobile)
+49 (0)351 210 2627 (Work phone at MPI-CBG)
+49 (0)351 210 1078 (Fax MPI-CBG LMF)
chalkie666 Skype
http://www.bioimagexd.net BioImageXD
http://fiji.sc Fiji - is just ImageJ (Batteries Included)
http://www.chalkie.org.uk Dan's Homepages
https://ifn.mpi-cbg.de Biopolis Dresden Imaging Platform (BioDIP)
dan (at) chalkie.org.uk
( white (at) mpi-cbg.de )
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