Re: Can someone Explain GDD and how it is adjusted? **Commercial Posting**

[Darryl McCoy]

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Dear List Users

The whitepaper we recently produced goes into some detail on this topic, and also into
areas such as peak power, average power and photodamage.  

It may be of some use to users not only choosing lasers (type – not brand!),  but
configuring their setups.  It can be downloaded here: http://goo.gl/SzGuaL

Any feedback or comments very welcome.

Best
Darryl


Darryl McCoy | Coherent Inc.
Acre Rd Glasgow, UK, G20 OXA | Phone: [phone]
New - Chameleon Discovery: One-box, wide tuning range, dual output laser for MPE.
http://www.coherent.com/chameleon

[cycylai]

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Dear microscopist,

I'm in a lab which also purchasing new multiphotons laser for intravital imaging. We are also in the dilemma of choosing laser of 140fs and 70fs which as the white paper suggest would have different fluorescent signal intensity and background. Can you the expert share your experience? Thanks very much.

Best regards,

Cyril Lai

Faculty Core Facility
LKS Faculty of Medicine, HKU


 ________________________________________
From: Confocal Microscopy List [[hidden email]] On Behalf Of Darryl McCoy [[hidden email]]
Sent: 18 December 2014 06:28
To: [hidden email]
Subject: Re: Can someone Explain GDD and how it is adjusted? **Commercial Posting**

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear List Users

The whitepaper we recently produced goes into some detail on this topic, and also into
areas such as peak power, average power and photodamage.

It may be of some use to users not only choosing lasers (type – not brand!),  but
configuring their setups.  It can be downloaded here: http://goo.gl/SzGuaL

Any feedback or comments very welcome.

Best
Darryl


Darryl McCoy | Coherent Inc.
Acre Rd Glasgow, UK, G20 OXA | Phone: [phone]
New - Chameleon Discovery: One-box, wide tuning range, dual output laser for MPE.
http://www.coherent.com/chameleon

[Craig Brideau]

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If you are also getting a pulse compressor, get the 70fs laser. if you are
not getting a pulse compressor then get the 140fs laser.

Craig
On Dec 17, 2014 6:47 PM, "cycylai" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear microscopist,
>
> I'm in a lab which also purchasing new multiphotons laser for intravital
> imaging. We are also in the dilemma of choosing laser of 140fs and 70fs
> which as the white paper suggest would have different fluorescent signal
> intensity and background. Can you the expert share your experience? Thanks
> very much.
>
> Best regards,
>
> Cyril Lai
>
> Faculty Core Facility
> LKS Faculty of Medicine, HKU
>
>
>  ________________________________________
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Darryl McCoy [[hidden email]]
> Sent: 18 December 2014 06:28
> To: [hidden email]
> Subject: Re: Can someone Explain GDD and how it is adjusted? **Commercial
> Posting**
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List Users
>
> The whitepaper we recently produced goes into some detail on this topic,
> and also into
> areas such as peak power, average power and photodamage.
>
> It may be of some use to users not only choosing lasers (type – not
> brand!),  but
> configuring their setups.  It can be downloaded here: http://goo.gl/SzGuaL
>
> Any feedback or comments very welcome.
>
> Best
> Darryl
>
>
> Darryl McCoy | Coherent Inc.
> Acre Rd Glasgow, UK, G20 OXA | Phone: [phone]
> New - Chameleon Discovery: One-box, wide tuning range, dual output laser
> for MPE.
> http://www.coherent.com/chameleon
>

[Andreas Bruckbauer]

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****


 It will also depend on the microscope setup. Our system contains an AOM an this contributes about 10,000 fs^2 to the overall GDD which is about 17000 fs^2. A 100 fs pulse would broaden to about 480 fs and a 70 fs pulse to 680 fs without pulse compensation. The spectra-physics Deepsee and Coherent Vision lasers have this built in but i can image that the pulse compensation has to be more carefully adjusted for the shorter pulse, but i have not used a 70 fs pulse yet.

best wishes

Andreas

 

 

-----Original Message-----
From: Craig Brideau <[hidden email]>
To: CONFOCALMICROSCOPY <[hidden email]>
Sent: Thu, 18 Dec 2014 9:12
Subject: Re: Can someone Explain GDD and how it is adjusted? **Commercial Posting**


*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

If you are also getting a pulse compressor, get the 70fs laser. if you are
not getting a pulse compressor then get the 140fs laser.

Craig
On Dec 17, 2014 6:47 PM, "cycylai" <[hidden email]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear microscopist,
>
> I'm in a lab which also purchasing new multiphotons laser for intravital
> imaging. We are also in the dilemma of choosing laser of 140fs and 70fs
> which as the white paper suggest would have different fluorescent signal
> intensity and background. Can you the expert share your experience? Thanks
> very much.
>
> Best regards,
>
> Cyril Lai
>
> Faculty Core Facility
> LKS Faculty of Medicine, HKU
>
>
>  ________________________________________
> From: Confocal Microscopy List [[hidden email]] On
> Behalf Of Darryl McCoy [[hidden email]]
> Sent: 18 December 2014 06:28
> To: [hidden email]
> Subject: Re: Can someone Explain GDD and how it is adjusted? **Commercial
> Posting**
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> Post images on http://www.imgur.com and include the link in your posting.
> *****
>
> Dear List Users
>
> The whitepaper we recently produced goes into some detail on this topic,
> and also into
> areas such as peak power, average power and photodamage.
>
> It may be of some use to users not only choosing lasers (type – not
> brand!),  but
> configuring their setups.  It can be downloaded here: http://goo.gl/SzGuaL
>
> Any feedback or comments very welcome.
>
> Best
> Darryl
>
>
> Darryl McCoy | Coherent Inc.
> Acre Rd Glasgow, UK, G20 OXA | Phone: [phone]
> New - Chameleon Discovery: One-box, wide tuning range, dual output laser
> for MPE.
> http://www.coherent.com/chameleon
>