** Vendor Reply **
Hi, Estaban,
Thermo Fisher / Molecular Probes sells the FocalCheck calibration slide #2, catalog F36913, which is made especially for spectral deconvolution instrument calibration and demonstrations. It has 6um diameter beads with two different, but closely overlapping, wavelengths of green, orange, red, and far red emitting beads, both labeled throughout in the first row, or ring/core combinations in the second row, as well as a mix of all eight beads in a separate region.
Web:
http://www.thermofisher.com/order/catalog/product/F36913Manual:
https://tools.thermofisher.com/content/sfs/manuals/mp36909.pdfPlease let me know if you need any further information on this.
Jason
Jason A. Kilgore
Technical Application Scientist
Molecular Probes Tech Support
Life Sciences Solutions
Thermo Fisher Scientific
29851 Willow Creek Rd.
Eugene, OR 97402-9132
1-800-955-6288 then option 4, then option 6, then option 2.
Or dial direct at +1 541 335 0353
[hidden email]
www.lifetechnologies.com
-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of G. Esteban Fernandez
Sent: Wednesday, January 13, 2016 10:50 AM
To:
[hidden email]
Subject: Commercial slide for spectral unmixing training
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Hello all,
I'd like to purchase a prepared slide containing spectrally overlapping fluorophores to train users on unmixing. Ideally it would contain cells or tissue stained with defined fluorophores, not inherent tissue fluorescence/"autofluorescence"). Does anyone know of a commercial source for a slide like that? (If not I can bum some cultured cells, buy reagents, and prepare slides myself.)
Thanks,
Esteban
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