Re: Commercial solutions for calibration of FRAP units

[Tom DiMatteo]

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** Comercial Posting**

Hi Anton,

The CaliCube is a commercial solution that can provide you with a quantitative
image of your FRAP light source, if you are using your epi illumination light
path for photo activation (typically non-confocal). By elimination fluorescing
components, this technique maximizes reproducibility and exposes
opportunities for human error, by providing a direct diagnostic view of the
conjugate plane of the epi light path. This image can then be quantitated by
your imaging system and used as a reference going forward. The CaliCube
comes standard with a light reduction capacity of greater than 1/10,000 and
can be custom ordered with higher ND values for greater exposure protection.
More information is available at http://www.epitechnology.com/.


Tom DiMatteo
Epi Technology
[hidden email]
415-235-4142


 On Mon, 1 Dec 2014 07:40:38 -0600, Anton Kamnev
<[hidden email]> wrote:

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>
>Dear all,
>
>In our LM core we face a problem of frequent calibration of our "FRAP"
>devices. Although the calibration itself is quite fast, many users just
>don't have good samples which could be used for this purpose.
>
>Therefore, I was wondering if there are any commercial products for
>calibration of bleaching devices? What I'm looking for is a slide with a
>thin uniform layer of a dye which could be bleached. The dye should be
>immobilized so a bleaching pattern would be observable for some period of
>time after the bleaching.
>
>I would really appreciate if you could share some experience about samples
>you use for calibration of bleaching patterns.
>
>Although I'm primarily interested in commercial solutions, I would very much
>appreciate some protocols for home-made calibration slides as well.
>
>Thanks in advance!
>Anton.
>
>---------------------------
>Anton Kamnev, PhD
>Imaging Manager
>Mechanochemical Cell Biology Building
>Division of Biomedical Cell Biology
>Warwick Medical School
>The University of Warwick
>Coventry, CV4 7AL UK
>
>tel: [phone]
>cell: [phone]
>email: [hidden email]
>
>Divisional Secretary: Karl Waldron
>Tel: [phone]
>[hidden email]