Hi Michal,
Just a thought. Whilst we're usually more interested in avoiding damage
than causing it, we can offer a quite strong and very controllable 365nm
LED.
I don't presently know if that wavelength is short enough to be
workable, nor if our source would be sufficiently intense, but if is
sounds feasible we may be able to offer a loan unit to try the
technique.
Kind regards,
Gordon.
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:
[hidden email]] On Behalf Of Michal Jarnik
> Sent: 21 June 2010 17:20
> To:
[hidden email]
> Subject: Controlled DNA damage by lasers
>
> Listers,
>
> We would need to perform experiments including controlled DNA
> damage to cells in culture. One idea was to use the LSCM and
> expose an area in a way similar to the bleaching part of a
> FRAP experiment. We do have a LED
> 405 nm laser (I would assume that would be the right
> wavelength, we do not have any really UV laser), Nikon C1 can
> do quite a lot of zooming to concentrate the energy in a
> small area. I have no idea, though, about the power
> necessary/available and other conditions. Any recommendations
> would be very much appreciated.
>
> Thanks, M.
>
> --
>
> Michal Jarnik, Ph.D.
> Cell Imaging Facility
> Electron Microscope Facility
> Fox Chase Cancer Center
> 333 Cottman Ave.
> Philadelphia, PA 19111
> 215-728-5675 (v.)
> 215-728-2770 (v.)
> 215-728-2412 (f.)
>
>
>
>
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