Re: Draq5 in yeast (tech support response)

[Roy Edward-2]

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Dear Sylvie,
It is certainly challenging to stain live yeast with any DNA dye.

Part of the issue is the complex cell wall. Spheroplasts will stain
relatively easily as will budding yeast; both with exposed plasma
membranes that will permit passage of a live cell-permeant dye.

You may wish to refer to the following article which might assist you:
Herrero et al. Appl. Environ. Microbiol. October 2006 vol. 72 no. 10
6725-6733
http://aem.asm.org/content/72/10/6725.short


In addition, there is a report of the use of DRAQ5 with 4mM EDTA to aid
entry into intact bacteria:
Silva et al. J Fluorescence July 2010, Volume 20, Issue 4, pp 907-914
http://link.springer.com/article/10.1007%2Fs10895-010-0636-y?LI=true


Don't hesitate to contact me directly if you have more questions regarding
this.
Med vennlig hilsen / Kinds regards,
Roy

Roy Edward

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On 02/02/2013 06:03, "CONFOCALMICROSCOPY automatic digest system"
<[hidden email]> wrote:

>
>----------------------------------------------------------------------
>
>Date:    Fri, 1 Feb 2013 13:37:26 +0000
>From:    Sylvie Le Guyader <[hidden email]>
>Subject: Draq5 in yeast
>
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>To join, leave or search the confocal microscopy listserv, go to:
>http://lists.umn.edu/cgi-bin/wa?A0=3Dconfocalmicroscopy
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>
>Dear all
>
>I would like to know if anyone has tried to stain yeast (Pombe) nuclei
>with=
> Draq5. We have used it in mammalian cells without problems but somehow
>we =
>did not get it to work in yeast.
>Any tip or any alternative far red nuclear stain?
>
>Cheers
>
>Med v=E4nlig h=E4lsning / Best regards
>
>Sylvie
>
>@@@@@@@@@@@@@@@@@@@@@@@@
>Sylvie Le Guyader
>Live Cell Imaging Unit
>Dept of Biosciences and Nutrition
>Karolinska Institutet
>H=E4lsov=E4gen 7
>14157 Huddinge
>Sweden
>office: [phone]
>LCI room: [phone]
>mobile: [phone]
>
>------------------------------