Re: [EXT] CONFOCALMICROSCOPY Digest - 2 Mar 2019 to 3 Mar 2019 (#2019-51)

[Jared K. Buks]

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I can state that Ivo is not alone, we have serious and ongoing dust issues as well and our system isn't that old.

Jared

Jared K. Burks, PhD
Associate Professor
Co-Director, Flow Cytometry & Cellular Imaging Core Facility
Department of Leukemia
M.D. Anderson Cancer Center Unit 0425
1515 Holcombe Blvd. Y8.5311a
Houston, TX  77030
713-792-6592

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Sent: Monday, March 4, 2019 12:01 AM
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Subject: [EXT] CONFOCALMICROSCOPY Digest - 2 Mar 2019 to 3 Mar 2019 (#2019-51)

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Topics of the day:

  1. Yokogawa CSU-W1

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Date:    Mon, 4 Mar 2019 02:04:35 +0000
From:    Manabu Fueki <[hidden email]>
Subject: Re: Yokogawa CSU-W1

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Dear Ivo,



Thank you very much for your patronage of our products.

I’m the QA manager of CSU series.

Would you please let me know the Serial No. of the CSU-W1?

And who is taking care of the dust problem?

I think we’ve deployed our latest countermeasure for the problem through our business partners.

If you have any problem, we will have to investigate the cause.



Best regards,

Manabu



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Manabu Fueki

Yokogawa Electric Corporation

Life Innovation Business HQ

QA & Customer Support Center

[hidden email]

2-3 Hokuyodai, Kanazawa-shi, Ishikawa, 920-0177 Japan

Tel:[phone]  Fax:[phone] Follow Us : Twitter<https://twitter.com/Yokogawa_LS> | Facebook<https://www.facebook.com/yokogawa.life.science/> | Linkedin<https://www.linkedin.com/company/yokogawa-life-science/>

https://www.yokogawa.com/solutions/products-platforms/life-science/

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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Ivo Telley
Sent: Friday, March 1, 2019 8:35 PM
To: [hidden email]
Subject: Re: Yokogawa CSU-W1



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Dear Sylvie



I wish to comment on this matter as we were one of the first purchasers of the W1 system. The dust problem has not been fixed to a satisfactory level for the W1 system. It is true that Yokogawa has “designed” and provided a fix, which essentially consists of a bunch of metal shields to try and compartmentalise better the interior of the scanner (i.e. electronics, ventilators, optics). However, in our experience the problem remains and after an intense cleaning by a technician the disk collects dust particles after 1-2 weeks, and we sometimes see bright auto-fluorescent particles in individual frames. The bright-spot problem gets worse within months and makes image processing unnecessary complicated.

I must stress here that this dust problem is caused by a suboptimal design of the entire optical compartment and its air sealing. This problem cannot be fixed easily.



Regards,

Ivo A. Telley, PhD

Instituto Gulbenkian de Ciência (IGC)

Rua da Quinta Grande, 6

2780-156 Oeiras, Portugal

Phone: [phone]

http://www.igc.gulbenkian.pt/itelley



> On 1 Mar 2019, at 11:22, Sylvie Le Guyader <[hidden email]<mailto:[hidden email]>> wrote:

>

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>

> Hi Paula

>

> I am interested in the dust problem you mention. My understanding is that this problem was fixed by Yokogawa and that they even apply their fix to older W1 for free if one complains.

> How old is your W1 spinning disk? Did you have to go through the fix or have you never had the problem?

>

> Another question regarding the pinhole cross talk: do you know how deep in your bead sample you were where you had negligible cross talk?

>

> thanks

>

> Med vänlig hälsning / Best regards

>

> Sylvie

>

> @@@@@@@@@@@@@@@@@@@@@@@@

> Sylvie Le Guyader, PhD

> Live Cell Imaging Facility Manager

> Karolinska Institutet- Bionut Dpt

> Hälsovägen 7C,

> Room 7362 (lab)/7840 (office)

> 14157 Huddinge, Sweden

> mobile: [phone]

> LCI website

> Follow our microscopy blog!

>

>

>

> -----Original Message-----

> From: Confocal Microscopy List
> <[hidden email]<mailto:[hidden email].
> EDU>> On Behalf Of Montero Llopis, Paula De La Milagrosa

> Sent: den 19 februari 2019 05:50

> To:
> [hidden email]<mailto:[hidden email]
> DU>

> Subject: Re: Yokogawa CSU-W1

>

> *****

> To join, leave or search the confocal microscopy listserv, go to:

> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy

> Post images on http://www.imgur.com and include the link in your posting.

> *****

>

> Dear Vitaly,

>

> We have a Yokowaga CSU-W1 on a Nikon Ti with an Andor Zyla 4.2 plus and are very happy with it. We have a Toptica 4 laser line laser launch that is easy to use and align. Pinhole crosstalk with the plan apo 100x/1.4 objective is negligible (at least with the bead samples we used to test it) and it does a great job even in lower NA lenses, at least compared to the CSU-X1. Setting it up was pretty straightforward.

>

> Dust is a bit of an issue, we have to wipe the dichroic mirrors once every couple of months. Haven't noticed as big issue with dust in the pinholes, but I think other people have reported this.

>

> Rolloff is not awful, but we also have a macro in place to crop the ROI, so that users don't have to think about it.

>

> We got our system through MVI/Nikon and they do a great job supporting it.

>

> I hope this helps!

>

> Best,

>

> Paula

>

> Paula Montero Llopis, PhD

> MicRoN Core Director

> micron.hms.harvard.edu

>

>> On Feb 15, 2019, at 08:13, Vitaly Boyko <[hidden email]<mailto:[hidden email]>> wrote:

>>

>> *****

>> To join, leave or search the confocal microscopy listserv, go to:

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>> Post images on http://www.imgur.com and include the link in your posting.

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>>

>>

>> Dear All,

>> We are looking for a feedback on the latest spinning disk confocal unit by Yokogawa. Any feedback on its performance, setup, stability, frequency of maintenance, quality of service will be appreciated.

>> Many thanks in advance.

>> Vitaly

>> MSKCCT: 212-639-6667

>>

>

>

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