Re: -|EXT|- shopping: live-sample confocal+super-res

[WILDE Geraint]

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*Commercial Response*

Dear Jeff,

Andor manufacture Dragonfly, a microlens-based spinning disk system (different design to the Yokogawa CSU), that supports a couple of super-resolution techniques.  If you wish to reach out to me directly then I would be more than happy to discuss our technology further with you.

Your sincerely

Geraint Wilde

Geraint Wilde Ph.D.
Product Manager, Life Science Cameras & Microscopy Systems  

Mob.    [phone]
Web     andor.oxinst.com


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Reece, Jeff (NIH/NIDDK) [E]
Sent: 26 April 2020 20:07
To: [hidden email]
Subject: -|EXT|- shopping: live-sample confocal+super-res


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Dear List,

We are a core facility ready to make a major purchase, seeking advice.  The system needs to provide fast, live-sample confocal imaging, but also super-res in the 100-150nm range (xy).  Here is a sampling of the applications we are trying to satisfy:

  1.  Z-stacks of cultured cells over time, multi-color labeled.  Super-res and standard confocal.
  2.  Z-stacks and/or time series of live tissue/organisms (e.g. c. elegans, oocytes) up to 40 microns deep (at least), multi-color labeled, super-res and standard confocal.
  3.  Z-stack, tile and stitch, super-res of fixed samples, e.g. FISH and tissue slices (e.g. mouse kidney).



We narrowed it down to the following instruments:

  1.  Nikon W1 SoRa spinning disk
  2.  Olympus W1 SoRa spinning disk ("SpinSR")
  3.  Visitech vt-iSIM (VisiView software seems to be the best choice here in the USA?)
  4.  Zeiss LSM 980 AiryScan 2
  5.  Zeiss Elyra 7 Lattice SIM


I will send another email for those that are theoretical-minded; for this email, I am interested in practical, hands-on impressions.
For any of you that have compared any of the above systems, I would greatly appreciate to hear those impressions, either to the list or directly to me.
Here are some common categories of comparison that may jog your memory and/or provide a framework for your response:

  1.  Resolution;
  2.  Speed;
  3.  Sensitivity;
  4.  Photobleaching;
  5.  Maintaining focal plane over time (all the vendors do this well now?);
  6.  Color-correction from blue to far red, to edge of image field;
  7.  Usability of software - i.e. user-friendliness, appropriate for a core facility;
  8.  Functionality-- i.e. range of features; capability to do what you need from a workflow/experimental point of view;
  9.  Reliability, robustness of the system;
  10. Customer support level.

Stay Safe and Healthy,
Jeff

Jeff Reece
Ph: +1.301.451.4330
E:  [hidden email]<https://mail.nih.gov/owa/14.3.174.1/scripts/premium/redir.aspx?C=vorg2hwQ3EG79HF4VARC2_-txi1AZNEITAaQhKx2WUBLeDOG3BM2dSsWeRsCBbyhbstXsPzU2G8.&URL=mailto%3ajeff.reece%40nih.gov>

Director, Advanced Light Microscopy & Image Analysis Core (ALMIAC) NIH (National Institutes of Health) /
   NIDDK (National Institute of Diabetes and Digestive and Kidney diseases)
8 Center Dr, Rm 126
Bethesda, MD
20892-0851

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