Re.:FRAP and TPE

["José A. Feijó"]

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we did GFP in a membrane protein, check Certal et al. (2008) Plant Cell,
//*20*: 614-634

some more hints on :
Feijó, JA, Moreno N (2004) two-photon microscopy applications to plant
cells. Protoplasma, 223:1-32

pro's: greater zz' precision on the FRAPing
con's: as always, go easy on the throttle of the laser, it's a tiny
border between FRAP and fry... plus, globally speaking, in our hands it
was never as vital as normal laser confocal. Still, in our case, the
extra precision was useful.

g'd luck

> From: Confocal Microscopy List [[hidden email]] On Behalf Of Julia Edgar [[hidden email]]
> Sent: Monday, March 12, 2012 12:48 PM
> To:[hidden email]
> Subject: FRAP and two-photon
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All
>
> Is anybody doing FRAP of EGFP or td tomato, using 2-photon microscopy? If so, would you be willing to share protocols/advice?
>
> Many thanks in advance.
> Julia Edgar

--


**********************************************************
Jose' A. Feijo', Professor Catedrático
----------------------------------------------------------
Dep. Biologia Vegetal, Fac.Ciencias, Universidade Lisboa
PT-1749-016 Lisboa, PORTUGAL

tel. [phone]/24, fax  [phone]

and/ e

Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL

tel. [phone]/19, fax [phone]
__________________________________________________________
e.mail:[hidden email]   [hidden email]

URL:http://www.igc.gulbenkian.pt/research/unit/38
orhttp://dbv.fc.ul.pt/users/jafeijo

**********************************************************

[Stéphane Pagès]

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There is a  paper by Thomas Oertner (Grunditz et all 2008 J. Neuro.) in
Switzerland in which the last figure is in vivo FRAP. They will probably be
able to help you,
Cheers,
Stéphane

Le 12 mars 2012 15:55, "José A. Feijó" <[hidden email]> a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
> *****
>
> we did GFP in a membrane protein, check Certal et al. (2008) Plant Cell,
> //*20*: 614-634
>
> some more hints on :
> Feijó, JA, Moreno N (2004) two-photon microscopy applications to plant
> cells. Protoplasma, 223:1-32
>
> pro's: greater zz' precision on the FRAPing
> con's: as always, go easy on the throttle of the laser, it's a tiny border
> between FRAP and fry... plus, globally speaking, in our hands it was never
> as vital as normal laser confocal. Still, in our case, the extra precision
> was useful.
>
> g'd luck
>
>
>
>  From: Confocal Microscopy List [[hidden email].**EDU<[hidden email]>]
>> On Behalf Of Julia Edgar [[hidden email]]
>> Sent: Monday, March 12, 2012 12:48 PM
>> To:CONFOCALMICROSCOPY@LISTS.**UMN.EDU<To%[hidden email]>
>> Subject: FRAP and two-photon
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/**wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy>
>> *****
>>
>> Dear All
>>
>> Is anybody doing FRAP of EGFP or td tomato, using 2-photon microscopy? If
>> so, would you be willing to share protocols/advice?
>>
>> Many thanks in advance.
>> Julia Edgar
>>
> --
>
>
> ************************************************************
> Jose' A. Feijo', Professor Catedrático
> ------------------------------**----------------------------
> Dep. Biologia Vegetal, Fac.Ciencias, Universidade Lisboa
> PT-1749-016 Lisboa, PORTUGAL
>
> tel. [phone]/24, fax  [phone]
>
> and/ e
>
> Inst.Gulbenkian Ciencia, PT-2780-156 Oeiras, PORTUGAL
>
> tel. [phone]/19, fax [phone]
> ______________________________**____________________________
> e.mail:[hidden email]   [hidden email]
>
> URL:http://www.igc.gulbenkian.**pt/research/unit/38<http://www.igc.gulbenkian.pt/research/unit/38>
> orhttp://dbv.fc.ul.pt/users/**jafeijo <http://dbv.fc.ul.pt/users/jafeijo>
>
> ************************************************************
>

--
Stéphane Pages PhD
Neurobiologie cellulaire
Centre de Recherche Université Laval Robert-Giffard (CRULRG)
www.greenspine.ca
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