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Hi Sarah
Why not try CyGEL and add a suitable fluorescing dye (or dyes) to give you the excitation at the wavelengths you mention?
We have used DNA dyes such as PI and DRAQ5 in the gel to successfully label of cells in this thermo-reversible hydrogel.
You may of course wish to choose dyes which are not going to get into your tissue / cells.
It will be important to ensure that the two fluors don't interact or quench though given the "distance" between them this should be less of an issue.
CyGEL itself does not autofluoresce in the visible range so the signal you get will only be that from the fluor added.
The dye will be added to the gel while it is still cold (ca. 8 deg C) and applied to the sample / slide and thereafter allowed to warm beyond 15 deg C when it will gel.
Upon chilling the gel will re-liquefy, repeatedly. Detailed info on CyGEL can be found at www.biostatus.com
Best regards and good luck
Roy
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Dear friends,
For my experiment I need to use a fluorescent gel which can excite with 407 nm and >700 nm .I appreciate if you help me with that.
Best
Sarah
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