Re: Fluorescent beads **vendor reply**

[Kilgore, Jason A.]

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** vendor reply **

Hi, Sylvie,

As Ulrike and others mentioned, we at Thermo Fisher Scientific do have several options to choose from in our FluoSpheres line of products. Table 1 in the product manual for FluoSpheres has a listing of the different colors and sizes offered in that range:
http://tools.thermofisher.com/content/sfs/manuals/mp05000.pdf

We also offer our PS-Speck beads, intended for point spread function determination, as catalog P7220, each microsphere has a diameter of 0.175 ± 0.005 µm (note the very small CV, for better reliability in sizing compared to the FluoSpheres products).
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07220.pdf

If you need more help in choosing, though, please feel free to email me separately from this thread and I'll be happy to help further.

Cheers,

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Boehm, Ulrike
Sent: Tuesday, April 6, 2021 6:58 PM
To: [hidden email]
Subject: Re: Fluorescent beads

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


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Dear Sylvie,

I would contact ThermoFisher and ask if they have FluoSpheres in green and red in size 40 nm or 100 nm in storage (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/summary-of-molecular-probes-fluospheres-fluorescent-microspheres.html).

Some of these beads are currently not listed on their website but believe it or not, you can still order them.

I have performed some of my Ph.D. experiments with Crimson 100 nm beads that do not exist, according to their website.

An entire custom-made batch costs approx. 12,000 Euro, but occasionally, labs order them custom-made, and ThermoFisher still might have some of these not listed beads in store.

Best of luck.

Best regards,

Ulrike

____________________

Ulrike Boehm, Ph.D.
Research Specialist | Advanced Imaging Center Howard Hughes Medical Institute | Janelia Research Campus
19700 Helix Drive, Ashburn, VA 20147
Email: [hidden email]
Phone: [phone] x1356



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Orestis Faklaris
Sent: Tuesday, April 6, 2021 4:05 PM
To: [hidden email]
Subject: Re: Fluorescent beads

External Email: Use Caution

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Hi,

apart from the existing and widespread FluoSpheres, we just received and are going to test and compare the PSF of the yellow 50-90 nm beads from SpheroTech (https://urldefense.com/v3/__https://www.spherotech.com/up_to_5.htm__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNye1cA4tU$  , number FP-00552-2).
We'll know more on their brightness the following days.

Best

Orestis


__________________________________
Orestis Faklaris, PhD
MRI, Montpellier Ressources Imagerie
CRBM-CNRS
1919 route de Mende
34293 Montpellier
FRANCE

phone: [phone]
https://urldefense.com/v3/__http://www.mri.cnrs.fr__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyCwDWy4s$
--

Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit :

> *****
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> *****
>
> Hello
>
> We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test.
> Does anyone know which beads are brightest?
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI
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>
>
>
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[Cromey, Douglas W - (dcromey)-2]

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Jason,

Has Thermo considered adding an NIR dye to the four-color tetraspec beads?

Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator
Dept. of Cellular & Molecular Medicine, University of Arizona
Life Sciences North, Room 463
1333 N. Martin Ave, Tucson, AZ  85721 USA

office:  LSN 463        email: [hidden email]
voice:  520-626-2824       fax:  [phone]

UA Microscopy Alliance - http://microscopy.arizona.edu/
A collaborative effort to bring information about shared microscopy
facilities to the University of Arizona and the community.

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kilgore, Jason A.
Sent: Wednesday, April 7, 2021 10:31 AM
To: [hidden email]
Subject: [EXT]Re: Fluorescent beads **vendor reply**

External Email

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** vendor reply **

Hi, Sylvie,

As Ulrike and others mentioned, we at Thermo Fisher Scientific do have several options to choose from in our FluoSpheres line of products. Table 1 in the product manual for FluoSpheres has a listing of the different colors and sizes offered in that range:
http://tools.thermofisher.com/content/sfs/manuals/mp05000.pdf

We also offer our PS-Speck beads, intended for point spread function determination, as catalog P7220, each microsphere has a diameter of 0.175 ± 0.005 µm (note the very small CV, for better reliability in sizing compared to the FluoSpheres products).
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07220.pdf

If you need more help in choosing, though, please feel free to email me separately from this thread and I'll be happy to help further.

Cheers,

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Boehm, Ulrike
Sent: Tuesday, April 6, 2021 6:58 PM
To: [hidden email]
Subject: Re: Fluorescent beads

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


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Dear Sylvie,

I would contact ThermoFisher and ask if they have FluoSpheres in green and red in size 40 nm or 100 nm in storage (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/summary-of-molecular-probes-fluospheres-fluorescent-microspheres.html).

Some of these beads are currently not listed on their website but believe it or not, you can still order them.

I have performed some of my Ph.D. experiments with Crimson 100 nm beads that do not exist, according to their website.

An entire custom-made batch costs approx. 12,000 Euro, but occasionally, labs order them custom-made, and ThermoFisher still might have some of these not listed beads in store.

Best of luck.

Best regards,

Ulrike

____________________

Ulrike Boehm, Ph.D.
Research Specialist | Advanced Imaging Center Howard Hughes Medical Institute | Janelia Research Campus
19700 Helix Drive, Ashburn, VA 20147
Email: [hidden email]
Phone: [phone] x1356



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Orestis Faklaris
Sent: Tuesday, April 6, 2021 4:05 PM
To: [hidden email]
Subject: Re: Fluorescent beads

External Email: Use Caution

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*****

Hi,

apart from the existing and widespread FluoSpheres, we just received and are going to test and compare the PSF of the yellow 50-90 nm beads from SpheroTech (https://urldefense.com/v3/__https://www.spherotech.com/up_to_5.htm__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNye1cA4tU$  , number FP-00552-2).
We'll know more on their brightness the following days.

Best

Orestis


__________________________________
Orestis Faklaris, PhD
MRI, Montpellier Ressources Imagerie
CRBM-CNRS
1919 route de Mende
34293 Montpellier
FRANCE

phone: [phone]
https://urldefense.com/v3/__http://www.mri.cnrs.fr__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyCwDWy4s$
--

Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit :

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> https://urldefense.com/v3/__http://lists.umn.edu/cgi-bin/wa?A0=confoca
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> https://urldefense.com/v3/__http://www.imgur.com__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNysq5N79E$  and include the link in your posting.
> *****
>
> Hello
>
> We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test.
> Does anyone know which beads are brightest?
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
> @@@@@@@@@@@@@@@@@@@@@@@@
> Sylvie Le Guyader, PhD
> Live Cell Imaging Facility Manager
> Karolinska Institutet- Bionut Dpt
> Blickagången 16,
> Room 7362 (lab)/7840 (office)
> 14157 Huddinge, Sweden
> mobile: [phone]
> LCI
> website<https://urldefense.com/v3/__https://ki.se/en/bionut/welcome-to
> -the-lci-facility__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3i
> wmDKKUhhgFxghw1jWNyuluB-64$ > Follow our microscopy
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> 5jQI$ >
>
>
>
>
> När du skickar e-post till Karolinska Institutet (KI) innebär detta att KI kommer att behandla dina personuppgifter. Här finns information om hur KI behandlar personuppgifter<https://urldefense.com/v3/__https://ki.se/medarbetare/integritetsskyddspolicy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyoHM2xQo$ >.
>
>
> Sending email to Karolinska Institutet (KI) will result in KI processing your personal data. You can read more about KI's processing of personal data here<https://urldefense.com/v3/__https://ki.se/en/staff/data-protection-policy__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNyhiLgZEc$ >.

[Kilgore, Jason A.]

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Not that I'm aware. But I've forwarded your suggestion to the product manager.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
 

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Cromey, Douglas W - (dcromey)
Sent: Wednesday, April 7, 2021 11:43 AM
To: [hidden email]
Subject: Re: Fluorescent beads **vendor reply**

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


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*****

Jason,

Has Thermo considered adding an NIR dye to the four-color tetraspec beads?

Doug

^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
Douglas W. Cromey, M.S. - Associate Scientific Investigator Dept. of Cellular & Molecular Medicine, University of Arizona Life Sciences North, Room 463
1333 N. Martin Ave, Tucson, AZ  85721 USA

office:  LSN 463        email: [hidden email]
voice:  520-626-2824       fax:  [phone]

UA Microscopy Alliance - https://urldefense.proofpoint.com/v2/url?u=http-3A__microscopy.arizona.edu_&d=DwIGaQ&c=q6k2DsTcEGCcCb_WtVSz6hhIl8hvYssy7sH8ZwfbbKU&r=MVp-2yJ1A-yQtCbZ-DE9xd0W6E7srQQpV-yioYjTLyQ&m=DdeBMDW9NkVdXkGcSQiiVLII93ihVH3Y3TBEt732GZ4&s=PQLm-mafVbl5sUKL8e9QC_1pkn77xFgxhK5UfpouxZs&e=
A collaborative effort to bring information about shared microscopy facilities to the University of Arizona and the community.

-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Kilgore, Jason A.
Sent: Wednesday, April 7, 2021 10:31 AM
To: [hidden email]
Subject: [EXT]Re: Fluorescent beads **vendor reply**

External Email

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** vendor reply **

Hi, Sylvie,

As Ulrike and others mentioned, we at Thermo Fisher Scientific do have several options to choose from in our FluoSpheres line of products. Table 1 in the product manual for FluoSpheres has a listing of the different colors and sizes offered in that range:
http://tools.thermofisher.com/content/sfs/manuals/mp05000.pdf

We also offer our PS-Speck beads, intended for point spread function determination, as catalog P7220, each microsphere has a diameter of 0.175 ± 0.005 µm (note the very small CV, for better reliability in sizing compared to the FluoSpheres products).
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp07220.pdf

If you need more help in choosing, though, please feel free to email me separately from this thread and I'll be happy to help further.

Cheers,

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions


-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Boehm, Ulrike
Sent: Tuesday, April 6, 2021 6:58 PM
To: [hidden email]
Subject: Re: Fluorescent beads

CAUTION: This email originated from outside of Thermo Fisher Scientific. If you believe it to be suspicious, report using the Report Phish button in Outlook or send to [hidden email].


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Dear Sylvie,

I would contact ThermoFisher and ask if they have FluoSpheres in green and red in size 40 nm or 100 nm in storage (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/tables/summary-of-molecular-probes-fluospheres-fluorescent-microspheres.html).

Some of these beads are currently not listed on their website but believe it or not, you can still order them.

I have performed some of my Ph.D. experiments with Crimson 100 nm beads that do not exist, according to their website.

An entire custom-made batch costs approx. 12,000 Euro, but occasionally, labs order them custom-made, and ThermoFisher still might have some of these not listed beads in store.

Best of luck.

Best regards,

Ulrike

____________________

Ulrike Boehm, Ph.D.
Research Specialist | Advanced Imaging Center Howard Hughes Medical Institute | Janelia Research Campus
19700 Helix Drive, Ashburn, VA 20147
Email: [hidden email]
Phone: [phone] x1356



-----Original Message-----
From: Confocal Microscopy List <[hidden email]> On Behalf Of Orestis Faklaris
Sent: Tuesday, April 6, 2021 4:05 PM
To: [hidden email]
Subject: Re: Fluorescent beads

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Hi,

apart from the existing and widespread FluoSpheres, we just received and are going to test and compare the PSF of the yellow 50-90 nm beads from SpheroTech (https://urldefense.com/v3/__https://www.spherotech.com/up_to_5.htm__;!!Eh6p8Q!RacJT6MFQZREzM0VgS723rZx8jF4mfPN6Td8xcV3iwmDKKUhhgFxghw1jWNye1cA4tU$  , number FP-00552-2).
We'll know more on their brightness the following days.

Best

Orestis


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Orestis Faklaris, PhD
MRI, Montpellier Ressources Imagerie
CRBM-CNRS
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34293 Montpellier
FRANCE

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Le 06/04/2021 à 15:27, Sylvie Le Guyader a écrit :

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> Hello
>
> We are looking for very bright, monocolour (green or red), 50-100 nm fluorescent beads for some PSF test.
> Does anyone know which beads are brightest?
>
> Med vänlig hälsning / Best regards
>
> Sylvie
>
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