Re: Fluorescent spheres - range 100um -1000um **Vendor Reply**

[Kilgore, Jason A.]

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** Vendor Reply **

Thermo Fisher Scientific offers the Fluoro-Max beads in sizes up to 140 um, in green or red emission, which is at least at the lower end of your desired size range.  See here:
http://www.thermoscientific.com/en/product/fluoro-max-green-red-dry-fluorescent-particles.html

Jason


Jason A. Kilgore
Technical Application Scientist
Molecular Probes Tech Support
Life Sciences Solutions

Thermo Fisher Scientific
29851 Willow Creek Rd.
Eugene, OR  97402-9132
1-800-955-6288 then option 4, then option 6, then option 2.
Or dial direct at [phone]
[hidden email]
www.lifetechnologies.com

This communication is intended solely for the individual/entity to whom it is addressed. It may contain confidential or legally privileged information.  Any unauthorized disclosure or copying is prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and delete it from your system.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jacqui Ross
Sent: Tuesday, April 19, 2016 9:36 PM
To: [hidden email]
Subject: Fluorescent spheres - range 100um -1000um

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Dear All,

Does anyone know of a company who can supply fluorescent spheres in this size range suitable for confocal imaging? I know people are usually looking for standards at the other end of the scale!

I've had a look at Bangs Laboratories and Spherotech but they don't seem to have anything large enough...

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/

[Smith, Benjamin E.]

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One thing to keep in mind, for most applications the image doesn't have to be a positive one (i.e. fluorescent spheres on a dark background).  With this in mind, you can mix a bit of fluorescent dye with some immersion oil, and mount some glass beads such as these:

http://www.sigmaaldrich.com/catalog/product/sigma/g1145?lang=en&region=US

Not only are glass beads cheap and come in a wide range of diameters, but they can also be used to prop up coverslips and keep them from crushing samples.

Hope this helps,
   Ben Smith

________________________________________
From: Confocal Microscopy List <[hidden email]> on behalf of Kilgore, Jason <[hidden email]>
Sent: Wednesday, April 20, 2016 12:13 PM
To: [hidden email]
Subject: Re: Fluorescent spheres - range 100um -1000um **Vendor Reply**

*****
To join, leave or search the confocal microscopy listserv, go to:
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*****

** Vendor Reply **

Thermo Fisher Scientific offers the Fluoro-Max beads in sizes up to 140 um, in green or red emission, which is at least at the lower end of your desired size range.  See here:
http://www.thermoscientific.com/en/product/fluoro-max-green-red-dry-fluorescent-particles.html

Jason


Jason A. Kilgore
Technical Application Scientist
Molecular Probes Tech Support
Life Sciences Solutions

Thermo Fisher Scientific
29851 Willow Creek Rd.
Eugene, OR  97402-9132
1-800-955-6288 then option 4, then option 6, then option 2.
Or dial direct at [phone]
[hidden email]
www.lifetechnologies.com

This communication is intended solely for the individual/entity to whom it is addressed. It may contain confidential or legally privileged information.  Any unauthorized disclosure or copying is prohibited and may be unlawful. If you have received this communication in error, please notify the sender immediately and delete it from your system.

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Jacqui Ross
Sent: Tuesday, April 19, 2016 9:36 PM
To: [hidden email]
Subject: Fluorescent spheres - range 100um -1000um

*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
Post images on http://www.imgur.com and include the link in your posting.
*****

Dear All,

Does anyone know of a company who can supply fluorescent spheres in this size range suitable for confocal imaging? I know people are usually looking for standards at the other end of the scale!

I've had a look at Bangs Laboratories and Spherotech but they don't seem to have anything large enough...

Kind regards,

Jacqui

Jacqueline Ross
Biomedical Imaging Microscopist
Biomedical Imaging Research Unit
School of Medical Sciences
Faculty of Medical & Health Sciences
The University of Auckland
Private Bag 92019
Auckland 1142, NEW ZEALAND

Tel: [phone]
Fax: [phone]

http://www.fmhs.auckland.ac.nz/sms/biru/