Re: General fluorescent stain for cytoplasm **vendor response**

[Kilgore, Jason A.]

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** Vendor Response **

Hi, Eric,

Just to expand on what a couple other commenters have said...   For live cells, the calcein AM dyes are the best option for filling the cytoplasm in live cells.  Molecular Probes / Thermo Fisher sells green, blue, violet, and red-orange variants.  Be aware they will be pumped out of the cells over time and are not fixable.

For fixability after live cell labeling, I would recommend the CellTracker or CellTrace dyes.  These will label cytoplasmic proteins (though not as uniform of a cytoplasmic labeling as calcein dyes).

Other options exist for cells that are already-fixed, but I won't go into those here since that doesn't seem to be the focus.

If you have more questions on these options, feel free to contact me separately.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes Tech Support
Life Sciences Solutions

Thermo Fisher Scientific
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Eric Shelden
Sent: Wednesday, September 17, 2014 10:49 AM
To: [hidden email]
Subject: General fluorescent stain for cytoplasm

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All:

I am looking for a way to fluorescently counter-stain the volume of cultured cells, with a result similar to what would be achieved by having them express GFP in the cytoplasm. I have seen some references to staining with eosin Y. Does anyone have other suggestions?

Thanks in advance.

Eric