Re: Gold Standard immersion oil
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Just doing some catching up on the listserv so sorry for the late reply, but
I would also recommend the 37oC oil from Cargille. It has RI 1.52 at 37oC. Claire |
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What about autofluorscence? Last time I tested this oil (which is
quite a while ago) I found that it had very high autofluorescence. Of course, this is much more critical for widefield than for confocal. Beat At 18:32 24-09-2008, you wrote: >Just doing some catching up on the listserv so sorry for the late reply, but >I would also recommend the 37oC oil from Cargille. It has RI 1.52 at 37oC. > >Claire |
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Hi all,
A quick update on the oil, I just tried out the DF from Cargille, again it has some fluorescence in the oil, its not too bad though but I will be trying the Olympus brand next - keep tuned! Neil Kad > Date: Wed, 24 Sep 2008 19:06:51 +0200 > From: [hidden email] > Subject: Re: Gold Standard immersion oil > To: [hidden email] > > What about autofluorscence? Last time I tested this oil (which is > quite a while ago) I found that it had very high autofluorescence. Of > course, this is much more critical for widefield than for confocal. > > Beat > > > At 18:32 24-09-2008, you wrote: > >Just doing some catching up on the listserv so sorry for the late reply, but > >I would also recommend the 37oC oil from Cargille. It has RI 1.52 at 37oC. > > > >Claire Win £3000 to spend on whatever you want at Uni! Click here to WIN! |
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Thanks Neil. However, my question referred to the
type 37 oil mentioned by Claire, not the type DF. In contrast to type DF (which is optimized for fluorescence microscopy), type 37 (which is optimized for 37°C) used to have very high autofluorescence and I wondered whether that had been improved in the meantime. At 10:35 25-09-2008, you wrote: >Hi all, > >A quick update on the oil, I just tried out the >DF from Cargille, again it has some fluorescence >in the oil, its not too bad though but I will be >trying the Olympus brand next - keep tuned! > >Neil Kad > > > Date: Wed, 24 Sep 2008 19:06:51 +0200 > > From: [hidden email] > > Subject: Re: Gold Standard immersion oil > > To: [hidden email] > > > > What about autofluorscence? Last time I tested this oil (which is > > quite a while ago) I found that it had very high autofluorescence. Of > > course, this is much more critical for widefield than for confocal. > > > > Beat > > > > > > At 18:32 24-09-2008, you wrote: > > >Just doing some catching up on the listserv > so sorry for the late reply, but > > >I would also recommend the 37oC oil from Cargille. It has RI 1.52 at 37oC. > > > > > >Claire > > >---------- >Win £3000 to spend on whatever you want at Uni! ><http://clk.atdmt.com/UKM/go/111354032/direct/01/>Click here to WIN! |
 
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Jason Swedlow |
Re: Gold Standard immersion oil
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Sorry to beat a dead horse, but the autofluorescence issue is why we used LaserLiquids by Cargille (see previous posting). A bit more money, but worth it, at least IMHO.
Cheers, Jason
On Thu, Sep 25, 2008 at 9:55 AM, Beat Ludin <[hidden email]> wrote: Thanks Neil. However, my question referred to the type 37 oil mentioned by Claire, not the type DF. In contrast to type DF (which is optimized for fluorescence microscopy), type 37 (which is optimized for 37°C) used to have very high autofluorescence and I wondered whether that had been improved in the meantime. |
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Hugo.Ostermann |
AW: Gold Standard immersion oil
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In reply to this post by Beat Ludin
Hi Listers,
I can proof this. The 37 Degree oils ha a much higher background than the Laser Fluid which has no measurable background. With kind regards Hugo Ostermann CHROMAPHOR Analysen-Technik GmbH Mühlenkamp 37 D-59387 Ascheberg - Germany Tel: (++49) 0172- 28 28 373 mail: [hidden email] URL: http://www.chromaphor.de -----Ursprüngliche Nachricht----- Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Beat Ludin Gesendet: Donnerstag, 25. September 2008 10:55 An: [hidden email] Betreff: Re: Gold Standard immersion oil Thanks Neil. However, my question referred to the type 37 oil mentioned by Claire, not the type DF. In contrast to type DF (which is optimized for fluorescence microscopy), type 37 (which is optimized for 37°C) used to have very high autofluorescence and I wondered whether that had been improved in the meantime. At 10:35 25-09-2008, you wrote: >Hi all, > >A quick update on the oil, I just tried out the >DF from Cargille, again it has some fluorescence >in the oil, its not too bad though but I will be >trying the Olympus brand next - keep tuned! > >Neil Kad > > > Date: Wed, 24 Sep 2008 19:06:51 +0200 > > From: [hidden email] > > Subject: Re: Gold Standard immersion oil > > To: [hidden email] > > > > What about autofluorscence? Last time I tested this oil (which is > > quite a while ago) I found that it had very high autofluorescence. Of > > course, this is much more critical for widefield than for confocal. > > > > Beat > > > > > > At 18:32 24-09-2008, you wrote: > > >Just doing some catching up on the listserv > so sorry for the late reply, but > > >I would also recommend the 37oC oil from Cargille. It has RI 1.52 at > > > > > >Claire > > >---------- >Win £3000 to spend on whatever you want at Uni! ><http://clk.atdmt.com/UKM/go/111354032/direct/01/>Click here to WIN! |
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Dear all,
Recently Cargille and we have developed and tested a new 37 degree oil for high-resolution live-cell fluorescence microscopy. This oil is based on the DF oil mentioned. The test results, including those on the level of autofluorescence, are described in a paper which will be published in a forthcoming issue of the J. Microscopy. If you want more information, please contact me off-list. I would like to add that I have hesitated a while to respond to this thread, since it might look like self-advertisement and a commercial response. However, I do not have any financial interest in the product or the company. Kind regards, Lauran -------------------------------------------------------------- Lauran Oomen Manager Digital Microscopy Facility (H003) NKI-AVL Plesmanlaan 121 PO Box 90203 1006 BE Amsterdam The Netherlands phone +31 205126080 fax +31 205122909 -----Original Message----- From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Hugo.Ostermann Sent: donderdag 25 september 2008 21:32 To: [hidden email] Subject: AW: Gold Standard immersion oil Hi Listers, I can proof this. The 37 Degree oils ha a much higher background than the Laser Fluid which has no measurable background. With kind regards Hugo Ostermann CHROMAPHOR Analysen-Technik GmbH Mühlenkamp 37 D-59387 Ascheberg - Germany Tel: (++49) 0172- 28 28 373 mail: [hidden email] URL: http://www.chromaphor.de -----Ursprüngliche Nachricht----- Von: Confocal Microscopy List [mailto:[hidden email]] Im Auftrag von Beat Ludin Gesendet: Donnerstag, 25. September 2008 10:55 An: [hidden email] Betreff: Re: Gold Standard immersion oil Thanks Neil. However, my question referred to the type 37 oil mentioned by Claire, not the type DF. In contrast to type DF (which is optimized for fluorescence microscopy), type 37 (which is optimized for 37°C) used to have very high autofluorescence and I wondered whether that had been improved in the meantime. At 10:35 25-09-2008, you wrote: >Hi all, > >A quick update on the oil, I just tried out the >DF from Cargille, again it has some fluorescence >in the oil, its not too bad though but I will be >trying the Olympus brand next - keep tuned! > >Neil Kad > > > Date: Wed, 24 Sep 2008 19:06:51 +0200 > > From: [hidden email] > > Subject: Re: Gold Standard immersion oil > > To: [hidden email] > > > > What about autofluorscence? Last time I tested this oil (which is > > quite a while ago) I found that it had very high autofluorescence. Of > > course, this is much more critical for widefield than for confocal. > > > > Beat > > > > > > At 18:32 24-09-2008, you wrote: > > >Just doing some catching up on the listserv > so sorry for the late reply, but > > >I would also recommend the 37oC oil from Cargille. It has RI 1.52 at > > > > > >Claire > > >---------- >Win £3000 to spend on whatever you want at Uni! ><http://clk.atdmt.com/UKM/go/111354032/direct/01/>Click here to WIN! |
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Hi Lauren,
This is great news. When will this be available? I'm also looking to know if they will have different refraction indices :) do you have a preprint you could send me?
best b
On 9/26/08, [hidden email] <[hidden email]> wrote: Dear all, -- Bruno Afonso http://brunoafonso.com (personal, mostly portuguese) http://openwetware.org/wiki/User:BrunoAfonso (Professional, english) |
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