Re: Has anyone used Konica Minolta fluorescent nanoparticles with cells or tissue sections? COMMERCIAL RESPONSE

[Jeffrey Carmichael]

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COMMERCIAL RESPONSE

George,
I haven't seen the nanoparticles, but regarding the BD Brilliant Violet
reference....

Chroma Technology does in fact offer optimized BV421 (49027) and BV480
(49032) filter sets.  The BV421 set allows for use of BV480 in the same
sample with no detectable spectral overlap (in our hands) and a slightly
red-shifted filter set intended for Alexa Fluor 488 (or similar) allows for
the use of BV421, BV480 and AF488.  The BV480 and AF488 fluorescence also
result in remarkably minimal spectral overlap when using these sets (not
detectable by eye)  BD Biosciences can provide some data.

BV421 filter set:
https://www.chroma.com/products/sets/49027-et-brilliant-violet-421-alexa-fluor-405?fluorochromes=18705
BV480 filter set:
https://www.chroma.com/products/sets/49032-et-brilliant-violet-480?fluorochromes=18706
AF488 (shifted filter set):
https://www.chroma.com/products/sets/49303-et-green1-fish?fluorochromes=10468

We also offer a 5-channel filter set with the 5-band dichroic and 5 separate
excitation and 5 emission filters for filter wheels, intended for this same
combination:
https://www.chroma.com/products/sets/89903-et-bv421-bv480-af488-af568-af647-quinta-band-set

In the next month we'll be offering a matching 5 band set for use with laser
excitation, with UltraFlat laser dichroic and 5-band ex. and 5-band em.
filters for use with lasers in the following ranges:
380-408nm, 442-455nm, 502-516nm, 559-575nm & 633-650nm

Here's a slide deck outlining the use of these BV fluors to enable a simple
5-color imaging scheme which is accessible without heroic efforts or
advanced expertise:
http://www.slideshare.net/JeffCarmichael/chr-5-color-imagingcopy

I'll also load the other BV and BUV spectra into our SpectraViewer.  Thanks
for pointing that out.  We're working on displaying the various fluors in
different colors for easier viewing.

Jeff


Jeff Carmichael
Technical and Product Marketing Mgr.
[hidden email]

Chroma Technology Corp.
an employee owned company
https://www.chroma.com/
10 Imtec Lane
Bellows Falls, VT 05101
1 802 428 2528 Voice Direct
1 802 428 2525 Fax
1 800 824 7662 ext. 2528 Toll Free


-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On
Behalf Of George McNamara
Sent: Monday, June 06, 2016 5:58 PM
To: [hidden email]
Subject: Has anyone used Konica Minolta fluorescent nanoparticles with cells
or tissue sections?

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Has anyone used Konica Minolta fluorescent nanoparticles with cells or
tissue sections ... and if yes, can they share the results (or cite
publication)?

http://www.konicaminolta.com/about/research/future/hstt/index.html

I came across this through a new press release (for some reason dated 2015),

http://www.konicaminolta.com/about/releases/2016/0606_01_01.html

on joint research agreement between Konica Minolta, Institut Pasteur, and
BioAxial (which I found that the Super-Resolution Microscopy
(Nanoscopy) group discussion).

I recently blogged on linkedin on an alternative family of fluorophores,

https://www.linkedin.com/pulse/bd-biosciences-listed-tandems-horizon-brilliant-violets-mcnamara

I note here that at a BD seminar last week the BD folks emphasized the
importance of using their Brilliant Staining Buffer (a tube comes in every
Brilliant antibody order). And yes, I've used BV421 successfully - very
bright on widefield (standard DAPI cube, would be even better with optimized
filter set), and does enable imaging on a confocal microscope.

thanks in advance,

George

--



George McNamara, Ph.D.
Houston, TX 77054
[hidden email]
713-239-0365 home
305-764-2081 cell
https://www.linkedin.com/in/georgemcnamara
https://works.bepress.com/gmcnamara/75/   Tattletales and T-Bow