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Hello,
The non-destructive immobilization of cells and objects for imaging can be
easily achieved using a patented, thermo-reversible hydrogel mountant
called CyGEL(TM).
In this thermo-reversible mountant, the sample can be recovered for
orthogonal analysis, be it culturing, sorting, DNA or RNA extraction, EM,
and so on.
In practice, CyGEL is cooled below RT to liquefy it and it can either be
mixed with or overlaid onto the cells/bacteria. A coverslip is then
placed on top.
The slide is cooled again to allow the gel to spread under the coverslip
and then wormed to set.
This should be good for a couple of hours without sealing the edges.
CyGEL is entirely compatible with fluorescence and can accept viability
dyes such as DRAQ7 to report dead cells.
You can see a review of this here:
www.nxtbook.com/nxtbooks/isc/abl_20100708/#/14
An example of its use in bacterial films is: Lakins et al. 2009 J
Microscopy 235 (2):128-137 (DOI: 10.1111/j.1365-2818.2009.03190.x)
Full technical and ordering info are to be found here: www.biostatus.com
Don't hesitate to contact me offline if you need further assistance.
Kind regards,
Roy
Roy Edward
E
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