Daniel James White |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Hi George, Can you send a link to the PDF of the Kasper paper, or the full reference, I dont find it ... cheers Dan On Sep 13, 2011, at 7:01 AM, CONFOCALMICROSCOPY automatic digest system wrote: > Bleaching during acquisition - check out the ROXS paper (Kasper et al > 2010, Small). This and similar reagents - including some for live cell > imaging - should improve STED, OMX (and the other 3D-SIM), and confocal, > and widefield imaging. Of course ultra-stable fluorophores would be > problematic for PALm/STORM/Monet (the user could always change solutions > for the latter modes). Dr. Daniel James White BSc. (Hons.) PhD Leader - Image Processing Facility, Senior Microscopist, Light Microscopy Facility. Max Planck Institute of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 DRESDEN Germany +49 (0)15114966933 (German Mobile) +49 (0)351 210 2627 (Work phone at MPI-CBG) +49 (0)351 210 1078 (Fax MPI-CBG LMF) chalkie666 Skype http://www.bioimagexd.net BioImageXD http://fiji.sc Fiji - is just ImageJ (Batteries Included) http://www.chalkie.org.uk Dan's Homepages https://ifn.mpi-cbg.de Biopolis Dresden Imaging Platform (BioDIP) dan (at) chalkie.org.uk ( white (at) mpi-cbg.de ) |
lechristophe |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Try this link : http://www.mpibpc.mpg.de/groups/hell/publications/pdf/smll_6_13_1379-1384.pdf Cheers, Christophe On Tue, Sep 13, 2011 at 09:55, Daniel James White <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Hi George, > > Can you send a link to the PDF of the Kasper paper, > or the full reference, I dont find it ... > > cheers > > Dan > > > On Sep 13, 2011, at 7:01 AM, CONFOCALMICROSCOPY automatic digest system > wrote: > > > Bleaching during acquisition - check out the ROXS paper (Kasper et al > > 2010, Small). This and similar reagents - including some for live cell > > imaging - should improve STED, OMX (and the other 3D-SIM), and confocal, > > and widefield imaging. Of course ultra-stable fluorophores would be > > problematic for PALm/STORM/Monet (the user could always change solutions > > for the latter modes). > > Dr. Daniel James White BSc. (Hons.) PhD > > Leader - Image Processing Facility, > Senior Microscopist, > Light Microscopy Facility. > > Max Planck Institute of Molecular Cell Biology and Genetics > Pfotenhauerstrasse 108 > 01307 DRESDEN > Germany > > +49 (0)15114966933 (German Mobile) > +49 (0)351 210 2627 (Work phone at MPI-CBG) > +49 (0)351 210 1078 (Fax MPI-CBG LMF) > chalkie666 Skype > http://www.bioimagexd.net BioImageXD > http://fiji.sc Fiji - is just ImageJ > (Batteries Included) > http://www.chalkie.org.uk Dan's Homepages > https://ifn.mpi-cbg.de Biopolis Dresden Imaging Platform > (BioDIP) > dan (at) chalkie.org.uk > ( white (at) mpi-cbg.de ) > |
George McNamara |
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To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Thanks Christophe, Daniel et al ... See also related articles such as, PubMed 21311807, 20121094. Just about all papers co-authored by Stefan Hell can be downloaded from his lab's web site: http://www.mpibpc.mpg.de/groups/hell/publications/publications.html Vicidomini et al 2011 time gating g-STED is (for now) the second reference in 2011 (g-STED has also been published as FLIM STED by other labs). His lab is falling behind in their web site - search PubMed (Hell SW) for more recent stuff like Urban et al; Wurm et al, Grotjohann et al. For more fun reading, google searches such as STED dissertation Hell turn up several PhD dissertations that (to my knowledge) are not available from the Hell lab site. Reuss (3D-EasySTED/g-STED): http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2011/11539/pdf/Reuss_Dissertation.pdf Engler (adaptive elements): http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2009/9941/pdf/AndreasEnglerDissertationOpt.pdf Giske (cryoSTED): http://archiv.ub.uni-heidelberg.de/volltextserver/volltexte/2008/7969/pdf/CryoSTED_microscopy_PhDThesis_agiske.pdf Google searches also turn up other fun reading, such as Deep STED (see also Urban et al 2011 Biophys J through PubMed): http://www.biop.dk/Documents/Berning_summary%20summerschool%20ven.pdf Lastly, a very fun read is: 1. A STED-y route to commercialization. </pubmed/21781034> Perkel JM. Biotechniques. 2011 Jun;50(6):357-63. No abstract available. PMID: 21781034 Enjoy, George On 9/13/2011 4:23 AM, Christophe Leterrier wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > ***** > > Try this link : > > http://www.mpibpc.mpg.de/groups/hell/publications/pdf/smll_6_13_1379-1384.pdf > > Cheers, > > Christophe > > On Tue, Sep 13, 2011 at 09:55, Daniel James White<[hidden email]> wrote: > > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> Hi George, >> >> Can you send a link to the PDF of the Kasper paper, >> or the full reference, I dont find it ... >> >> cheers >> >> Dan >> >> >> On Sep 13, 2011, at 7:01 AM, CONFOCALMICROSCOPY automatic digest system >> wrote: >> >> >>> Bleaching during acquisition - check out the ROXS paper (Kasper et al >>> 2010, Small). This and similar reagents - including some for live cell >>> imaging - should improve STED, OMX (and the other 3D-SIM), and confocal, >>> and widefield imaging. Of course ultra-stable fluorophores would be >>> problematic for PALm/STORM/Monet (the user could always change solutions >>> for the latter modes). >>> >> Dr. Daniel James White BSc. (Hons.) PhD >> >> Leader - Image Processing Facility, >> Senior Microscopist, >> Light Microscopy Facility. >> >> Max Planck Institute of Molecular Cell Biology and Genetics >> Pfotenhauerstrasse 108 >> 01307 DRESDEN >> Germany >> >> +49 (0)15114966933 (German Mobile) >> +49 (0)351 210 2627 (Work phone at MPI-CBG) >> +49 (0)351 210 1078 (Fax MPI-CBG LMF) >> chalkie666 Skype >> http://www.bioimagexd.net BioImageXD >> http://fiji.sc Fiji - is just ImageJ >> (Batteries Included) >> http://www.chalkie.org.uk Dan's Homepages >> https://ifn.mpi-cbg.de Biopolis Dresden Imaging Platform >> (BioDIP) >> dan (at) chalkie.org.uk >> ( white (at) mpi-cbg.de ) >> >> > -- George McNamara, PhD Analytical Imaging Core Facility University of Miami |
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