Re: Leica SP8 confocal Windows 10 upgrade
Locked
 
|
Arnim Jenett |
Re: Leica SP8 confocal Windows 10 upgrade
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear confocalmicroscopy-list, please allow me to reactivate this old thread because the problem persists: How to update the operating systems of Leica SP8 from Windows7 (end of life) to Windows10. The (only) solution, which was recently offered to us by Leica, is to purchase a new workstation for ~9KEuro. And our team is not alone with this problem as I know at least 6 other microscopes with this issue. Did this group find an alternative solution to this problem? Did anybody successfully upgrade the OS on the existing hardware - without breaking the system? Any input on this is highly appreciated. cheers, aRnim -- Dr. Arnim Jenett Imaging and Data Management TEFOR Paris-Saclay, CNRS UMS2010 / INRAE UMS1451, Université Paris-Saclay Bat 32, 1 Avenue de la Terrasse 91190 Gif-sur-Yvette, France https://tps.tefor.net |
|
|
Zdenek Svindrych-2 |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Arnim, first, I'm *not* a happy owner of SP8. Second, with most (if not all) of our microscopes we're happier with Windows 7. It is unsupported, but it works; why break it then? When IT folks are complaining, my philosophy is, if a smart refrigerator, chinese watch, or a phone with Android 4 can connect to the institution's WiFi, why not a Win 7 PC? Third, if you don't get a more assuring answer from other listers, you can just "give it a shot": 1) Get a new harddrive (assuming your PC does not support NVMe I'd suggest a SATA SSD with good TBW specs, e.g. ADATA SU800, or, even better, Samsung 860 PRO (not EVO)). 2) now you have two options - upgrade the OS (higher chances of success?) or start from scratch (cleaner system?) 2a) To upgrade, clone your old disk to the SSD (it's a bit tricky if your old disk is bigger than the SSD). 3a) Boot from the SSD and do an in-place upgrade to Windows 10 (some versions are tricky to upgrade, e.g. Win 7 Enterprise). 4a) Test and report! or 2b) To start from scratch, install clean Windows 10 on the SSD (I remember some LAS versions could only run from the D drive, so if that's your case, and you want LAS to run from the same SSD, just partition it accordingly). 3b) Install the same LAS software you used under Windows 7 (the LAS may want to rewrite the firmware of the SP8, so if you use the same version of LAS, you'll be able to go back to your old OS later, if needed). 4b) Test and report! Good luck! zdenek On Wed, Oct 21, 2020 at 5:50 AM Arnim Jenett <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear confocalmicroscopy-list, > please allow me to reactivate this old thread because the problem > persists: How to update the operating systems of Leica SP8 from Windows7 > (end of life) to Windows10. > The (only) solution, which was recently offered to us by Leica, is to > purchase a new workstation for ~9KEuro. And our team is not alone with this > problem as I know at least 6 other microscopes with this issue. > > Did this group find an alternative solution to this problem? Did anybody > successfully upgrade the OS on the existing hardware - without breaking the > system? > > Any input on this is highly appreciated. > cheers, aRnim > > -- > Dr. Arnim Jenett > Imaging and Data Management > TEFOR Paris-Saclay, > CNRS UMS2010 / INRAE UMS1451, > Université Paris-Saclay > > Bat 32, 1 Avenue de la Terrasse > 91190 Gif-sur-Yvette, France > https://tps.tefor.net > -- -- Zdenek Svindrych, Ph.D. Research Scientist - Microscopy Imaging Specialist Department of Biochemistry and Cell Biology Geisel School of Medicine at Dartmouth |
|
|
Craig Brideau |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Our IT department created a "walled off" section of our network for our Win7 microscopes. We can connect our microscope computers to our local server for data transfer, but they are cut off from everything else and will reject outside connections. It's not a perfect solution, but our IT department deemed it "good enough" given our needs and inability to upgrade them to Win10. Craig On Wed, Oct 21, 2020 at 7:27 AM Zdenek Svindrych <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Arnim, > > first, I'm *not* a happy owner of SP8. > > Second, with most (if not all) of our microscopes we're happier with > Windows 7. It is unsupported, but it works; why break it then? When IT > folks are complaining, my philosophy is, if a smart refrigerator, chinese > watch, or a phone with Android 4 can connect to the institution's WiFi, why > not a Win 7 PC? > > Third, if you don't get a more assuring answer from other listers, you can > just "give it a shot": > 1) Get a new harddrive (assuming your PC does not support NVMe I'd suggest > a SATA SSD with good TBW specs, e.g. ADATA SU800, or, even better, Samsung > 860 PRO (not EVO)). > 2) now you have two options - upgrade the OS (higher chances of success?) > or start from scratch (cleaner system?) > 2a) To upgrade, clone your old disk to the SSD (it's a bit tricky if your > old disk is bigger than the SSD). > 3a) Boot from the SSD and do an in-place upgrade to Windows 10 (some > versions are tricky to upgrade, e.g. Win 7 Enterprise). > 4a) Test and report! > or > 2b) To start from scratch, install clean Windows 10 on the SSD (I remember > some LAS versions could only run from the D drive, so if that's your case, > and you want LAS to run from the same SSD, just partition it accordingly). > 3b) Install the same LAS software you used under Windows 7 (the LAS may > want to rewrite the firmware of the SP8, so if you use the same version of > LAS, you'll be able to go back to your old OS later, if needed). > 4b) Test and report! > > Good luck! > > zdenek > > On Wed, Oct 21, 2020 at 5:50 AM Arnim Jenett <[hidden email]> wrote: > > > ***** > > To join, leave or search the confocal microscopy listserv, go to: > > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > > Post images on http://www.imgur.com and include the link in your > posting. > > ***** > > > > Dear confocalmicroscopy-list, > > please allow me to reactivate this old thread because the problem > > persists: How to update the operating systems of Leica SP8 from Windows7 > > (end of life) to Windows10. > > The (only) solution, which was recently offered to us by Leica, is to > > purchase a new workstation for ~9KEuro. And our team is not alone with > this > > problem as I know at least 6 other microscopes with this issue. > > > > Did this group find an alternative solution to this problem? Did anybody > > successfully upgrade the OS on the existing hardware - without breaking > the > > system? > > > > Any input on this is highly appreciated. > > cheers, aRnim > > > > -- > > Dr. Arnim Jenett > > Imaging and Data Management > > TEFOR Paris-Saclay, > > CNRS UMS2010 / INRAE UMS1451, > > Université Paris-Saclay > > > > Bat 32, 1 Avenue de la Terrasse > > 91190 Gif-sur-Yvette, France > > https://tps.tefor.net > > > > > -- > -- > Zdenek Svindrych, Ph.D. > Research Scientist - Microscopy Imaging Specialist > Department of Biochemistry and Cell Biology > Geisel School of Medicine at Dartmouth > |
|
|
kspencer007 |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by Arnim Jenett
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Bonjour Arnim; We have a very clunky but inexpensive work-around, but it works. This is to address our IT department encouraging elimination of Win 7 computers for network security. We found a second low-end computer and installed Win 10 Pro. Then we connected this directly to our Win 7 computer with a StarTech.com USB 3.0 to Gigabit Ethernet NIC Network Adapter. Our users have to transfer their acquired data from the Win 7 computer to the Win 10 computer at the end of their session (easy drag-and-drop). The Win 10 computer is accessible for remote data transfer. Best; Kathy -----Original Message----- From: Confocal Microscopy List <[hidden email]> On Behalf Of Arnim Jenett Sent: Wednesday, October 21, 2020 2:39 AM To: [hidden email] Subject: Re: Leica SP8 confocal Windows 10 upgrade ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear confocalmicroscopy-list, please allow me to reactivate this old thread because the problem persists: How to update the operating systems of Leica SP8 from Windows7 (end of life) to Windows10. The (only) solution, which was recently offered to us by Leica, is to purchase a new workstation for ~9KEuro. And our team is not alone with this problem as I know at least 6 other microscopes with this issue. Did this group find an alternative solution to this problem? Did anybody successfully upgrade the OS on the existing hardware - without breaking the system? Any input on this is highly appreciated. cheers, aRnim -- Dr. Arnim Jenett Imaging and Data Management TEFOR Paris-Saclay, CNRS UMS2010 / INRAE UMS1451, Université Paris-Saclay Bat 32, 1 Avenue de la Terrasse 91190 Gif-sur-Yvette, France https://tps.tefor.net |
|
|
Blaise Ndjamen-2 |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by Arnim Jenett
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Armin, Our IT Dept opted for a Win 7 "Extended Security Updates (ESU)" from Microsoft inc through 2023. So far we had no problem and the cost is very reasonable. For more information on how to obtain the ESU, please check out this blog: " https://techcommunity.microsoft.com/t5/windows-it-pro-blog/obtaining-extended-security-updates-for-eligible-windows-devices/ba-p/1167091 )". I hope this helps. Best, Blaise Blaise Ndjamen, PhD Director, Histology and Light Microscopy Core Facility, Gladstone Institutes, 1650 Owens St, San Francisco, CA 94158 Tel. 415-734-2566 https://labs.gladstone.org/histology/index.html On Wed, Oct 21, 2020 at 2:50 AM Arnim Jenett <[hidden email]> wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear confocalmicroscopy-list, > please allow me to reactivate this old thread because the problem > persists: How to update the operating systems of Leica SP8 from Windows7 > (end of life) to Windows10. > The (only) solution, which was recently offered to us by Leica, is to > purchase a new workstation for ~9KEuro. And our team is not alone with this > problem as I know at least 6 other microscopes with this issue. > > Did this group find an alternative solution to this problem? Did anybody > successfully upgrade the OS on the existing hardware - without breaking the > system? > > Any input on this is highly appreciated. > cheers, aRnim > > -- > Dr. Arnim Jenett > Imaging and Data Management > TEFOR Paris-Saclay, > CNRS UMS2010 / INRAE UMS1451, > Université Paris-Saclay > > Bat 32, 1 Avenue de la Terrasse > 91190 Gif-sur-Yvette, France > https://tps.tefor.net > |
|
|
In reply to this post by Arnim Jenett
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear Arnim, Steffen Dietzel for example was one of the people who bravely did the upgrade by themselves and he published the procedure which addresses Leica SP8: https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html From Win7 to Win 10 LTSC - Core Facility Bioimaging - LMU Munich<https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html> SP8 with compact supply unit with a HP Z640 ().SP8 MP plus compact supply unit and Camera DFC365FX with a HP Z640, LASX 3.5.0.18371 ().SP8 WLL with a HP Z640, LASX 3.5.5.19976 (). www.bioimaging.bmc.med.uni-muenchen.de It's a bit of hacking around. My former colleague followed the procedure and got stuck. Luckily, the Leica representatives there are quite flexible (not a Leica subsidiary but a third party company) and their engineer helped with the upgrade, so it ended successfully. Best wishes, Radek Radek MACHAN, Ph.D. (Senior Research Fellow) SCELSE Advanced Biofilm Imaging Facility<http://www.scelse.sg/Page/imaging-facility> Manager Nanyang Technological University #B2, 60 Nanyang Drive, SBS-01N-27 Singapore 637551 ________________________________ From: Confocal Microscopy List <[hidden email]> on behalf of Arnim Jenett <[hidden email]> Sent: Wednesday, October 21, 2020 17:39 To: [hidden email] <[hidden email]> Subject: Re: Leica SP8 confocal Windows 10 upgrade ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear confocalmicroscopy-list, please allow me to reactivate this old thread because the problem persists: How to update the operating systems of Leica SP8 from Windows7 (end of life) to Windows10. The (only) solution, which was recently offered to us by Leica, is to purchase a new workstation for ~9KEuro. And our team is not alone with this problem as I know at least 6 other microscopes with this issue. Did this group find an alternative solution to this problem? Did anybody successfully upgrade the OS on the existing hardware - without breaking the system? Any input on this is highly appreciated. cheers, aRnim -- Dr. Arnim Jenett Imaging and Data Management TEFOR Paris-Saclay, CNRS UMS2010 / INRAE UMS1451, Université Paris-Saclay Bat 32, 1 Avenue de la Terrasse 91190 Gif-sur-Yvette, France https://tps.tefor.net ________________________________ CONFIDENTIALITY: This email is intended solely for the person(s) named and may be confidential and/or privileged. If you are not the intended recipient, please delete it, notify us and do not copy, use, or disclose its contents. Towards a sustainable earth: Print only when necessary. Thank you. |
|
|
Arnim Jenett |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by Arnim Jenett
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear All, thank you very much for the multitude of good ideas/solutions. When I find some time we will dive into the approach of the LMU - integrating the dd (and avoiding the frimware update) as suggested by Zdenek. The short-term solution - at least for our systems - however, will be based on Kathy's suggestion: as our Microscopes already are directly connected to a Linux file server which is pulling the data automatically from the microscopes, we will simply take the SP8s offline and access the files exclusively through the server. Thank you for opening my eyes, there! I'll report back on the progress/success (much later) so others can profit from our experiences as I will from these of/at the LMU. Gratefully, aRnim PS: we are already running the ESU on these machines. Good point. -------- Forwarded Message -------- Subject: Re: Leica SP8 confocal Windows 10 upgrade Date: Wed, 21 Oct 2020 04:39:26 -0500 From: Arnim Jenett <[hidden email]> To: [hidden email] CC: Arnim Jenett <[hidden email]> Dear confocalmicroscopy-list, please allow me to reactivate this old thread because the problem persists: How to update the operating systems of Leica SP8 from Windows7 (end of life) to Windows10. The (only) solution, which was recently offered to us by Leica, is to purchase a new workstation for ~9KEuro. And our team is not alone with this problem as I know at least 6 other microscopes with this issue. Did this group find an alternative solution to this problem? Did anybody successfully upgrade the OS on the existing hardware - without breaking the system? Any input on this is highly appreciated. cheers, aRnim -- Dr. Arnim Jenett Imaging and Data Management TEFOR Paris-Saclay, CNRS UMS2010 / INRAE UMS1451, Université Paris-Saclay Bat 32, 1 Avenue de la Terrasse 91190 Gif-sur-Yvette, France https://tps.tefor.net |
|
|
Nino Karpf |
Software Recommendation for 3D volume registration/alignment
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Dear list, I’m looking for a software to align two 3D volumes. Basically, I am scanning volumes bi-directionally and there is a hysteresis between the up and downward motion. Now I’m looking for a software/ImageJ plug-in to correct this hysteresis. So far we have tested: https://imagej.net/Descriptor-based_registration_(2d/3d) <https://imagej.net/Descriptor-based_registration_(2d/3d)> and https://imagej.net/Fijiyama <https://imagej.net/Fijiyama> However, we haven’t had the results we anticipated. The former only worked for 2D images for us. Anyone has experience with this and can recommend us a good software? Perhaps even with a tutorial Video (e.g. the first plug in above has a nice tutorial: https://www.youtube.com/watch?v=SKW1xwhsxdo <https://www.youtube.com/watch?v=SKW1xwhsxdo>). Thank you! Nino Karpf __________________________________ Prof. Dr. Sebastian 'Nino' Karpf Juniorprofessor University of Lübeck Institute for Biomedical Optics (BMO) Tel +49 451 3101 3240 E-Mail [hidden email] <mailto:[hidden email]> www.bmo.uni-luebeck.de <http://www.bmo.uni-luebeck.de/> Peter-Monnik-Weg 4 23562 Lübeck Germany |
|
|
Heddleston, John-2 |
Re: [BULK][EXT] Software Recommendation for 3D volume registration/alignment
|
|
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Nino, Alignment of 3D volumes can be done in FIJI, but is a little tricky since FIJI can only represent 3D data as stacks of slices. So if your volumes have to be aligned in XYZ and have shear/mag issues, FIJI will require a lot of trial and error and could still result in slice to slice variation. Is this something you have to perform regularly or a one-off issue? If you want really effective N-D alignment I would recommend either going the hard route and coding it in Matlab or Python, or using a purchased software package like Imaris. If you want to keep to free software, BigWarp in FIJI can do a great job but is mainly main for 2D images. FIJIyama or the other built-in registration plugins are probably your best options. Cheers, John ____________________________________________________________________________ John M. Heddleston | Director | Microscopy Facility Cleveland Clinic | Florida Research & Innovation Center Email: [hidden email] Desk: 772.345.8117 From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Sebastian 'Nino' Karpf Sent: Thursday, October 22, 2020 6:11 AM To: [hidden email] Subject: [BULK][EXT] Software Recommendation for 3D volume registration/alignment ***** To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy<http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy> Post images on http://www.imgur.com<http://www.imgur.com> and include the link in your posting. ***** Dear list, I’m looking for a software to align two 3D volumes. Basically, I am scanning volumes bi-directionally and there is a hysteresis between the up and downward motion. Now I’m looking for a software/ImageJ plug-in to correct this hysteresis. So far we have tested: https://imagej.net/Descriptor-based_registration_(2d/3d)<https://imagej.net/Descriptor-based_registration_(2d/3d)> <https://imagej.net/Descriptor-based_registration_(2d/3d)<https://imagej.net/Descriptor-based_registration_(2d/3d)>> and https://imagej.net/Fijiyama<https://imagej.net/Fijiyama> <https://imagej.net/Fijiyama<https://imagej.net/Fijiyama>> However, we haven’t had the results we anticipated. The former only worked for 2D images for us. Anyone has experience with this and can recommend us a good software? Perhaps even with a tutorial Video (e.g. the first plug in above has a nice tutorial: https://www.youtube.com/watch?v=SKW1xwhsxdo<https://www.youtube.com/watch?v=SKW1xwhsxdo> <https://www.youtube.com/watch?v=SKW1xwhsxdo<https://www.youtube.com/watch?v=SKW1xwhsxdo>>). Thank you! Nino Karpf __________________________________ Prof. Dr. Sebastian 'Nino' Karpf Juniorprofessor University of Lübeck Institute for Biomedical Optics (BMO) Tel +49 451 3101 3240 E-Mail [hidden email]<mailto:[hidden email]> <mailto:[hidden email]> www.bmo.uni-luebeck.de<http://www.bmo.uni-luebeck.de> <http://www.bmo.uni-luebeck.de/<http://www.bmo.uni-luebeck.de>> Peter-Monnik-Weg 4 23562 Lübeck Germany Please consider the environment before printing this e-mail Cleveland Clinic is currently ranked as the No. 2 hospital in the country by U.S. News & World Report (2017-2018). Visit us online at http://www.clevelandclinic.org for a complete listing of our services, staff and locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. |
|
|
Vincent Schoonderwoert |
Re: Software Recommendation for 3D volume registration/alignment - vendor reply
|
|
In reply to this post by Nino Karpf
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** ** vendor reply ** Dear Nino, Two, or even more, 3D volumes with similar structures can be automatically aligned by treating them as different channels within our Huygens Chromatic Aberration Corrector. See https://svi.nl/Chromatic-Aberration-Corrector-Software This tool estimates and corrects shifts, rotational differences and changes in magnification. Typically these parameters are to be estimated from a known structure like a multicolored bead, and then applied to the biological image data acquired under the same conditions. You can contact me offline for more info and a trial version. Good luck, Vincent Vincent Schoonderwoert, PhD Director Marketing & Science - imaging Specialist Scientific Volume Imaging bv Laapersveld 63, 1213VB Hilversum, The Netherlands Phone: +31 35 642 1626 [hidden email] On 22-10-2020 12:10, Sebastian 'Nino' Karpf wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear list, > > I’m looking for a software to align two 3D volumes. Basically, I am scanning volumes bi-directionally and there is a hysteresis between the up and downward motion. Now I’m looking for a software/ImageJ plug-in to correct this hysteresis. > > So far we have tested: > > https://imagej.net/Descriptor-based_registration_(2d/3d) <https://imagej.net/Descriptor-based_registration_(2d/3d)> > > and > > https://imagej.net/Fijiyama <https://imagej.net/Fijiyama> > > However, we haven’t had the results we anticipated. The former only worked for 2D images for us. > > Anyone has experience with this and can recommend us a good software? Perhaps even with a tutorial Video (e.g. the first plug in above has a nice tutorial: https://www.youtube.com/watch?v=SKW1xwhsxdo <https://www.youtube.com/watch?v=SKW1xwhsxdo>). > > Thank you! > Nino Karpf > > > > __________________________________ > Prof. Dr. Sebastian 'Nino' Karpf > Juniorprofessor > > > University of Lübeck > Institute for Biomedical Optics (BMO) > > Tel +49 451 3101 3240 > E-Mail [hidden email] <mailto:[hidden email]> > www.bmo.uni-luebeck.de <http://www.bmo.uni-luebeck.de/> > > Peter-Monnik-Weg 4 > 23562 Lübeck > Germany Email Signature |
|
|
Steffen Dietzel |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by ramachan
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Radek, glad to hear it worked out for you at the end. Yes, "a bit of hacking around" describes it quite appropriately, I am afraid. Backup is essential, so that you can go back to the starting point, if everything else should fail. BTW, should you plan to upgrade to the linear scanning electronics (Klondike Upgrade), that would come with a Win10 upgrade included (and possibly new computer), since it does not run on Win7. From memory, it increases the duty cycle in unidirectional mode from 25% to 65% (i.e. the percentage of the total time during which photons are collected.) With the same line frequency and pixel size, pixel dwell time goes up. For some, it may be easier to convince funders to pay for 2.6x more photons in the same time period (or respectively shorter acquisition times), rather than a windows upgrade, although it is likely to be several times more expensive than what Arnim mentioned as Win10 upgrade costs. But I totally agree with others that fencing Win7 systems off the internet with a gateway computer is an efficient, fast and relatively inexpensive way to solve the problem in many settings. I did that once with a Win XP system hidden behind a NAS which had several Ethernet ports, one to the outside net, and one to the microscope computer. That worked very nicely. Steffen Am 22.10.2020 um 02:59 schrieb Radek Machan (Dr): > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear Arnim, > > Steffen Dietzel for example was one of the people who bravely did the > upgrade by themselves and he published the procedure which addresses > Leica SP8: > > https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html > >From Win7 to Win 10 LTSC - Core Facility Bioimaging - LMU > Munich<https://www.bioimaging.bmc.med.uni-muenchen.de/manuals-protocols/maintenance_protocolls/win7towin10/index.html> > SP8 with compact supply unit with a HP Z640 ().SP8 MP plus compact > supply unit and Camera DFC365FX with a HP Z640, LASX 3.5.0.18371 > ().SP8 WLL with a HP Z640, LASX 3.5.5.19976 (). > www.bioimaging.bmc.med.uni-muenchen.de > > It's a bit of hacking around. My former colleague followed the > procedure and got stuck. Luckily, the Leica representatives there are > quite flexible (not a Leica subsidiary but a third party company) and > their engineer helped with the upgrade, so it ended successfully. > > Best wishes, > Radek > > > Radek MACHAN, Ph.D. (Senior Research Fellow) > SCELSE Advanced Biofilm Imaging > Facility<http://www.scelse.sg/Page/imaging-facility> Manager > Nanyang Technological University > #B2, 60 Nanyang Drive, SBS-01N-27 > Singapore 637551 > ________________________________ > From: Confocal Microscopy List <[hidden email]> on > behalf of Arnim Jenett <[hidden email]> > Sent: Wednesday, October 21, 2020 17:39 > To: [hidden email] <[hidden email]> > Subject: Re: Leica SP8 confocal Windows 10 upgrade > > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Dear confocalmicroscopy-list, > please allow me to reactivate this old thread because the problem > persists: How to update the operating systems of Leica SP8 from > Windows7 (end of life) to Windows10. > The (only) solution, which was recently offered to us by Leica, is to > purchase a new workstation for ~9KEuro. And our team is not alone with > this problem as I know at least 6 other microscopes with this issue. > > Did this group find an alternative solution to this problem? Did > anybody successfully upgrade the OS on the existing hardware - without > breaking the system? > > Any input on this is highly appreciated. > cheers, aRnim > > -- > Dr. Arnim Jenett > Imaging and Data Management > TEFOR Paris-Saclay, > CNRS UMS2010 / INRAE UMS1451, > Université Paris-Saclay > > Bat 32, 1 Avenue de la Terrasse > 91190 Gif-sur-Yvette, France > https://tps.tefor.net > ________________________________ > > CONFIDENTIALITY: This email is intended solely for the person(s) named > and may be confidential and/or privileged. If you are not the intended > recipient, please delete it, notify us and do not copy, use, or > disclose its contents. > Towards a sustainable earth: Print only when necessary. Thank you. -- ------------------------------------------------------------ Steffen Dietzel, PD Dr. rer. nat Ludwig-Maximilians-Universität München Biomedical Center (BMC) Head of the Core Facility Bioimaging Großhaderner Straße 9 D-82152 Planegg-Martinsried Germany http://www.bioimaging.bmc.med.uni-muenchen.de |
|
|
Ben Abrams-2 |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by Blaise Ndjamen-2
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** Hi Blaise, I heard about the ESU option from one of your colleagues and had been exploring it when this thread came up, great timing. I am curious if you or anyone else who has gone the ESU route has had any issues with the updates interfering with the microscope control software at all? I know that sometimes security updates can include changes to the .net framework which I've been warned about in the past. Is this a valid concern? Thanks, Ben On 10/21/20 11:22 AM, Blaise Ndjamen wrote: > ***** > To join, leave or search the confocal microscopy listserv, go to: > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy > Post images on http://www.imgur.com and include the link in your posting. > ***** > > Hi Armin, > Our IT Dept opted for a Win 7 "Extended Security Updates (ESU)" from > Microsoft inc through 2023. So far we had no problem and the cost is very > reasonable. For more information on how to obtain the ESU, please check out > this blog: " > https://techcommunity.microsoft.com/t5/windows-it-pro-blog/obtaining-extended-security-updates-for-eligible-windows-devices/ba-p/1167091 > )". > I hope this helps. > > Best, > Blaise > > Blaise Ndjamen, PhD > > Director, > > Histology and Light Microscopy Core Facility, > > Gladstone Institutes, > > 1650 Owens St, San Francisco, CA 94158 > > Tel. 415-734-2566 > > https://labs.gladstone.org/histology/index.html > > > On Wed, Oct 21, 2020 at 2:50 AM Arnim Jenett <[hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> Post images on http://www.imgur.com and include the link in your posting. >> ***** >> >> Dear confocalmicroscopy-list, >> please allow me to reactivate this old thread because the problem >> persists: How to update the operating systems of Leica SP8 from Windows7 >> (end of life) to Windows10. >> The (only) solution, which was recently offered to us by Leica, is to >> purchase a new workstation for ~9KEuro. And our team is not alone with this >> problem as I know at least 6 other microscopes with this issue. >> >> Did this group find an alternative solution to this problem? Did anybody >> successfully upgrade the OS on the existing hardware - without breaking the >> system? >> >> Any input on this is highly appreciated. >> cheers, aRnim >> >> -- >> Dr. Arnim Jenett >> Imaging and Data Management >> TEFOR Paris-Saclay, >> CNRS UMS2010 / INRAE UMS1451, >> Université Paris-Saclay >> >> Bat 32, 1 Avenue de la Terrasse >> 91190 Gif-sur-Yvette, France >> https://tps.tefor.net >> -- Benjamin Abrams, Ph.D. (He/Him/His) Director UCSC Life Sciences Microscopy Center University of California, Santa Cruz 1156 High Street 150 Sinsheimer labs Santa Cruz, CA 95064 Office/voicemail: (831) 459-3999 Mobile: (831) 332-0911 http://stemcell.soe.ucsc.edu/facilities/microscopy Training Request Form: http://goo.gl/forms/M9jd1ZHyohTnj0xk1 |
|
|
Benjamin Smith |
Re: Software Recommendation for 3D volume registration/alignment
|
|
Claire Brown |
Re: Leica SP8 confocal Windows 10 upgrade
|
|
In reply to this post by Arnim Jenett
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy Post images on http://www.imgur.com and include the link in your posting. ***** We also went with extended Windows 7 security. We contacted the university IT and we didn't even have to pay for it. The university had purchased limited extended licenses. Was a quick fix! |
«
Return to Confocal Microscopy List
|
1 view|%1 views
| Free forum by Nabble | Edit this page |