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I have successfully use both HEPES buffer media and better yet Leibovitz's L-15 Medium
Rich
Richard Cole
Director: Advanced Light Microscopy & Image Analysis Core
Research Assistant Professor, Dept. of Biomedical Sciences
Wadsworth Center
120 New Scotland Ave, Albany N.Y. 12208
518-474-7048 Phone
Website www.wadsworth.org/cores/alm/index.htm