Re: LysoSensor and LysoTracker **Vendor reply**

[Kilgore, Jason A.]

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** Vendor reply **

Hi, Feriel,

It's an important question.

I've used the LysoTracker dyes many times and haven't seen any signs of cellular toxicity in the timeframes of my imaging experiments, though I don't think I've ever imaged beyond about an hour or so.

I don't have references to the effect, but our internal researchers (Nick Dolman and Yu-Zhong Zhang, who originally helped develop these dyes) looked at this question and performed some controlled experimentation.  I've cc'd them with their approval.

Here's how they responded:

Low dose they aren't toxic, and they aren’t retained well for the time periods that would cause toxicity. Though all organic dyes can perturb in some manner if you incubate for long periods or high concentrations, or over-expression (in the case of fluorescent proteins). Using the LysoTrackers as they are intended doesn’t give conditions that are generally toxic. As always the researcher should control for any experimental manipulation, including label.

Please let us know if you need further information.

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Thermo Fisher Scientific
 
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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Feriel P
Sent: Tuesday, July 03, 2018 8:28 AM
To: [hidden email]
Subject: LysoSensor and LysoTracker

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Hello everyone,

I am looking for papers that have evaluated the degree of toxicity of LysoSensor and LysoTracker Probes. I was unable to find details on the product pages or the references they mention. I have not yet used these probes, but I am aware that the mechanism of action is that they work by being protonated and retained in lysosomes. I have been told that these probes can exhibit an alkalinizing effect on the lysosomes, such that longer incubations increase pH and lower total lysosomal capacity.

Does anyone have references they have turned to in the past that elucidate the mechanism of LysoSensor and LysoTracker Probes and the toxic effects of prolonged use in live cell imaging? I'd be very grateful if you could direct me to author(s) or papers that have studied this.

Many thanks,
-Feriel
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