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** Vendor Response **
Hi, Claire,
Which MitoTracker Red are you using? MitoTracker Red FM, or MitoTracker Red CMXRos?
What is your label time and concentration?
What are the cells in at the time of imaging?
Jason
Jason A. Kilgore
Technical Application Scientist
Molecular Probes / EVOS Tech Support
Life Sciences Solutions
Thermo Fisher Scientific
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From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Claire Brown, Dr.
Sent: Tuesday, September 27, 2016 9:10 AM
To: [hidden email]
Subject: MitoTracker Red and Fixation
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Hi All,
We are trying to fix some cells with MitoTracker Red. We usually use it live but were trying to do some photobleaching experiments. The dye seems to be quenching or washing out when we fix the cells. We tried
using methanol free PFA and fixing at 37oC as the Probes protocol suggests.
Any suggestions?
Sincerely,
Claire
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