Re: Nuclear stains and Tissue Clearing **Vendor Reply**

[Kilgore, Jason A.]

** Vendor Reply **

Just to clarify, Qnuclear Deep Red hasn't been validated with samples that have been BABB or DBE-cleared by us here at Molecular Probes, so we don't know how compatible it is with those treatments.  It will work on standard cryosections or paraffin sections, as well as cells.  Yves is correct that it is compatible with subsequent dehydration with organic solvents, but I would add that it will work without such treatment as well.

It doesn't require a special mounting medium to work.  (Qmount is now discontinued, and replaced with the Image-iT Non-Aqueous Mounting medium (I36969), which will also work with the dye.)

Other red or far red nucleic acid stains for nuclear labeling in tissue can include TO-PRO-3, 7-AAD, and NucRed Dead, though these haven't been validated with BABB or DBE-cleared samples, either.

If anyone has questions about these options, feel free to contact me offline.

Best wishes,

Jason


Jason A. Kilgore
Technical Application Scientist
Molecular Probes Tech Support
Life Sciences Solutions

Thermo Fisher Scientific
29851 Willow Creek Rd.
Eugene, OR  97402-9132
1-800-955-6288 then option 4, then option 6, or
[phone]
[hidden email]
www.lifetechnologies.com

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Yves LUTZ
Sent: Friday, July 11, 2014 8:25 AM
To: [hidden email]
Subject: Re: Nuclear stains and Tissue Clearing

Hi Donald,

Have you tried Q nuclear deep red stain from Life Tech designed in the QDot field (ref Q10363)?  It needs a dehydrated sample as for clearing with BABB or DBE (although perhaps as well a  special mounting medium called Q mount, ref Q 10336).

I my clearing experiments with BABB or DBE, the use of Hoechst 33342 (10-50 ug/ml) was not too bad.

To mention that non dehydrating clearing procedures work well with Hoechst ( e.g.SeeDB).

Best regards,

Yves


Dr Yves Lutz PhD
Imaging Centre - IGBMC
1, rue Laurent Fries
67404 Illkirch
FRANCE






-----Message d'origine-----
De : Confocal Microscopy List [mailto:[hidden email]] De la part de Donald Alexander Fowler Envoyé : jeudi 10 juillet 2014 19:34 À : [hidden email] Objet : Nuclear stains and Tissue Clearing

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Dear Confocal Microscopy,

Does anybody know of a good nuclear stain that will stay specific to the nuclei when cleared in BABB or Dibenzyl ether?  I've tried in embryonic chick tissue and have had very poor results.

Kind Regards,

Donald

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Donald Alexander Fowler
PhD Candidate
Department of Biology
McGill University