Re: Reflex microscope - commercial interest -

[Klughammer Industrie GmbH]

- commercial interest -

We developed a software which takes images (macroscope, microscope)
and renders a stack of images to a 3D image. The images may be
captured manually (not so exact), or with a motorized system. Any
microscope can be used. The software asks for the distance between the
stack images. The 3D image can be rotated. The surface may be
measured.

Have a look at www.klughammer.de > products > software > 3D

Kind regards

Anneliese Schmaus LLB (hons)
Product Manager
____________________
Klughammer Bio GmbH
Strassbach 9
85229 Markt Indersdorf
Germany

Tel. [phone]
Fax [phone]
[hidden email]
www.klughammer.de


2009/1/22 Stanislav Vitha <[hidden email]>:

> I have done topographical imaging of tooth replicas, made with fluorescently
> stained dental epoxy.  I have used Olympus FV1000 and the 40x/0.6 long
> working distance objective; Fluorescence mode worked better than the
> reflective mode in my case, because the surface reflectivity was very uneven.
>
> Topographical projections were made in ImageJ with TopoJ plugin - this gave
> a better output than the TOPO projection function built in the Olympus
> confocal software.
>
> The results were OK; I have not measured the z-resolution of the
> topographical map, but my guesstimate is that it is somewhere between 20
> and 100 nm.  An example (of the reflective mode and a 3D test specimen) is
> shown on our web site: http://microscopy.tamu.edu/picture-of-the-
> month.html (scorll down to April 2008).
> The biggest problem was the speed of acquisition - the need to scan large
> areas with lots of pixels, plus doing 50 nm or smaller z-step over several mm
> depth quickly made it impractical or impossible to do imaging for anything more
> than just a handful of samples.
>
> The spinning-disk confocal profilometers are much much faster. besides the
> Sensofar system mentioned before, I know that Zeiss and Nanofocus have
> such spinning disk systems.
>
> Regards,
>  Stan
>
> Stanislav Vitha, Ph.D.
> Microscopy and Imaging Center
> Texas A&M University
> BSBW 119
> College Station, TX 77843-2257
>
> http://microscopy.tamu.edu
>
> On Wed, 21 Jan 2009 18:56:38 +0530, regan m <[hidden email]> wrote:
>
>>care should be executed while sample preparation on what is the resolution
>>you are looking for if u neeed a higher resolution your working distance is
>>low cut the sample enough from the bottom so that u get max magnification
>>without compromising on resolution.
>>
>>On Wed, Jan 21, 2009 at 4:16 PM, Xavier Sanjuan
> <[hidden email]>wrote:
>>
>>> I think any of the confocals in the market have a reflection mode and are
>>> capable of getting a topographical image of a surface with this. At least
>>> topographical images of silicone skin replicas has been done with a
>>> standard
>>> Leica SP2.
>>>
>>> There are also more specialised equipment to do this, like the PLµ confocal
>>> profilometer by Sensofar, http://www.sensofar.com/products.html (no
>>> commercial interest)
>>>
>>> Best,
>>>
>>> Xavi.
>>>
>>> ___________________________________
>>>
>>> Xavier Sanjuan
>>> Servei de Microscòpia Confocal
>>> Departament de Ciències Experimentals i de la Salut
>>> Universitat Pompeu Fabra
>>> Parc de Recerca Biomèdica de Barcelona
>>> Doctor Aiguader, 88 - Sala 309
>>> 08003 Barcelona - Spain
>>>
>>> Tel.:  + 34 93 316 08 64
>>> Fax: + 34 93 316 09 01
>>> E-mail: [hidden email]
>>> Web: http://www.upf.edu/sct
>>>
>>> -----Mensaje original-----
>>> De: Confocal Microscopy List
> [mailto:[hidden email]] En
>>> nombre de Carl Boswell
>>> Enviado el: martes, 20 de enero de 2009 18:59
>>> Para: [hidden email]
>>> Asunto: Reflex microscope
>>>
>>> I know someone at Arizona State University (Phoenix) that is looking for a
>>> Reflex microscope to look at surface details of dental specimens.  Anyone
>>> with experience with one, and know where one might be found?
>>>
>>> Thanks,
>>> carl
>>>
>>> Carl A. Boswell, Ph.D.
>>> Molecular and Cellular Biology
>>> University of Arizona
>>> 520-954-7053
>>> FAX [phone]
>>>
>>
>