*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Just for general information, I came across this note about TDE as a precursor for mustard gas. Can anyone verify this?: http://cbwinfo.com/General/Proliferation/Thiodiglycol.html On Wed, Jun 19, 2013 at 5:54 PM, JOEL B. SHEFFIELD <[hidden email]> wrote: > Hi Doug, > > A significant problem in imaging through the layers of the retina is that > some of the structures that you may be looking at are, themselves, in > overlapping layers. Thus one nucleus in the inl may very well block proper > access to one that is deeper in the tissue. That problem aside, the other > problem is the contribution of all of the plexiform layers to the optical > path. Over the years, we have been able to circumvent this by mounting in > various concentrations of glycerol, which might match much of the > refractive index of the fiber layers. We have also begun to look at the > effect of 2,2 thiodiethanol, which does wonders for cells in culture, > although it seems to extract certain lipophylic dyes. see: Staudt T, et > al (2006). "2,2′-Thiodiethanol: A new water soluble mounting medium for > high resolution optical microscopy". *Microscopy Research and Technique* * > 70*: 1–9. doi <http://en.wikipedia.org/wiki/Digital_object_identifier>: > 10.1002/jemt.20396 <http://dx.doi.org/10.1002%2Fjemt.20396> > > Although I'm sure you're aware of it, you should also keep in mind that > the psf can be quite elongated in the z direction, and might yield > misleading results. Often, if we're not careful, spheres end up appearing > like tubes when viewed from the side. > > Joel > > > > > On Wed, Jun 19, 2013 at 5:29 PM, Cromey, Douglas W - (dcromey) < > [hidden email]> wrote: > >> ***** >> To join, leave or search the confocal microscopy listserv, go to: >> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy >> ***** >> >> I have a lab that is starting a project that will involve confocal >> imaging of whole mount retinas (small rodents). We were able to image >> to-pro3 labeled nuclei through several layers to a depth of approximately >> 100um using a 63x water immersion objective. >> >> Is there anyone that does something similar that would be willing to >> offer up some sample prep tips? >> >> I recognize that spherical aberration becomes a significant issue when >> imaging that deep. Any suggestions for possible clearing techniques >> (hopefully something not too complicated)? >> >> Doug >> >> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ >> Douglas W. Cromey, M.S. - Associate Scientific Investigator >> Dept. of Cellular & Molecular Medicine, University of Arizona >> 1501 N. Campbell Ave, Tucson, AZ 85724-5044 USA >> >> office: AHSC 4212 email: [hidden email] >> voice: 520-626-2824 fax: 520-626-2097 >> >> http://swehsc.pharmacy.arizona.edu/micro >> Home of: "Microscopy and Imaging Resources on the WWW" >> > > > > -- > > > Joel B. Sheffield, Ph.D > Department of Biology > Temple University > Philadelphia, PA 19122 > Voice: 215 204 8839 > e-mail: [hidden email] > URL: http://astro.temple.edu/~jbs > -- Joel B. Sheffield, Ph.D Department of Biology Temple University Philadelphia, PA 19122 Voice: 215 204 8839 e-mail: [hidden email] URL: http://astro.temple.edu/~jbs |
*****
To join, leave or search the confocal microscopy listserv, go to: http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy ***** Yes it is. Be careful. M On 19/06/2013, at 10:58 PM, "JOEL B. SHEFFIELD" <[hidden email]> wrote: >> 2,2 thiodiethanol Mark B. Cannell Ph.D. FRSNZ Professor of Cardiac Cell Biology School of Physiology & Pharmacology Medical Sciences Building University of Bristol Bristol BS8 1TD UK [hidden email] |
Free forum by Nabble | Edit this page |