Re: Thiodiethanol

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JOEL B. SHEFFIELD JOEL B. SHEFFIELD
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Re: Thiodiethanol

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Just for general information, I came across this note about TDE as a
precursor for mustard gas.  Can anyone verify this?:

http://cbwinfo.com/General/Proliferation/Thiodiglycol.html

On Wed, Jun 19, 2013 at 5:54 PM, JOEL B. SHEFFIELD <[hidden email]> wrote:

> Hi Doug,
>
> A significant problem in imaging through the layers of the retina is that
> some of the structures that you may be looking at are, themselves, in
> overlapping layers.  Thus one nucleus in the inl may very well block proper
> access to one that is deeper in the tissue.  That problem aside, the other
> problem is the contribution of all of the plexiform layers to the optical
> path.  Over the years, we have been able to circumvent this by mounting in
> various concentrations of glycerol, which might match much of the
> refractive index of the fiber layers.  We have also begun to look at the
> effect of 2,2 thiodiethanol, which does wonders for cells in culture,
> although it seems to extract certain lipophylic dyes.  see:  Staudt T, et
> al (2006). "2,2′-Thiodiethanol: A new water soluble mounting medium for
> high resolution optical microscopy". *Microscopy Research and Technique* *
> 70*: 1–9. doi <http://en.wikipedia.org/wiki/Digital_object_identifier>:
> 10.1002/jemt.20396 <http://dx.doi.org/10.1002%2Fjemt.20396>
>
> Although I'm sure you're aware of it, you should also keep in mind that
> the psf can be quite elongated in the z direction, and might yield
> misleading results.  Often, if we're not careful, spheres end up appearing
> like tubes when viewed from the side.
>
> Joel
>
>
>
>
> On Wed, Jun 19, 2013 at 5:29 PM, Cromey, Douglas W - (dcromey) <
> [hidden email]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> I have a lab that is starting a project that will involve confocal
>> imaging of whole mount retinas (small rodents).  We were able to image
>> to-pro3 labeled nuclei through several layers to a depth of approximately
>> 100um using a 63x water immersion objective.
>>
>> Is there anyone that does something similar that would be willing to
>> offer up some sample prep tips?
>>
>> I recognize that spherical aberration becomes a significant issue when
>> imaging that deep.  Any suggestions for possible clearing techniques
>> (hopefully something not too complicated)?
>>
>> Doug
>>
>> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
>> Douglas W. Cromey, M.S. - Associate Scientific Investigator
>> Dept. of Cellular & Molecular Medicine, University of Arizona
>> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>>
>> office:  AHSC 4212         email: [hidden email]
>> voice:  520-626-2824       fax:  520-626-2097
>>
>> http://swehsc.pharmacy.arizona.edu/micro
>> Home of: "Microscopy and Imaging Resources on the WWW"
>>
>
>
>
> --
>
>
> Joel B. Sheffield, Ph.D
> Department of Biology
> Temple University
> Philadelphia, PA 19122
> Voice: 215 204 8839
> e-mail: [hidden email]
> URL:  http://astro.temple.edu/~jbs
>



--


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [hidden email]
URL:  http://astro.temple.edu/~jbs
Mark Cannell-2 Mark Cannell-2
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Re: Thiodiethanol

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Yes it is. Be careful.

M


On 19/06/2013, at 10:58 PM, "JOEL B. SHEFFIELD" <[hidden email]> wrote:

>> 2,2 thiodiethanol

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology &  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

[hidden email]